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. 2014 Mar 14;169(3-4):171–178. doi: 10.1016/j.vetmic.2014.01.003

Multidrug-resistant Escherichia coli from canine urinary tract infections tend to have commensal phylotypes, lower prevalence of virulence determinants and ampC-replicons☆

Samuel Wagner 1, David L Gally 1, Sally A Argyle 1,⁎
PMCID: PMC3969583  PMID: 24485933

Abstract

Multidrug-resistant Escherichia coli is an emerging clinical challenge in domestic species. Treatment options in many cases are limited. This study characterized MDR E. coli isolates from urinary tract infections in dogs, collected between 2002 and 2011. Isolates were evaluated in terms of β-lactamase production, phylogenetic group, ST type, replicon type and virulence marker profile. Comparisons were made with antibiotic susceptible isolates also collected from dogs with urinary tract infections. AmpC β-lactamase was produced in 67% of the MDR isolates (12/18). Of these, 8 could be specifically attributed to the CMY-2 gene. None of the isolates tested in either group expressed ESBLs. Phylo-group distribution was as expected in the susceptible isolates, with an over representation of the pathogenic B2 phylo-group (67%). In contrast, the phylogenetic background for the MDR group was mixed, with representation of commensal phylo-groups A and B1. The B2 phylo-group represented the smallest proportion (A, B1, B2 or D was 28%, 22%, 11% and 33%, respectively). Virulence marker profiles, evaluated using Identibac® microarray, discriminated between the two groups. Marker sequences for a core panel of virulence determinants were identified in most of the susceptible isolates, but not in most of the MDR isolates. These findings indicate that for MDR isolates, plasmid-mediated AmpC is an important resistance mechanism, and while still capable of causing clinical disease, there is evidence for a shift towards phylogenetic groups of reduced inferred virulence potential. There was no evidence of zoonotic potential in either the susceptible or MDR urinary tract isolates in this study.

Keywords: Escherichia coli, Plasmid-mediated AmpC, Urinary tract infection, Dog, Multidrug-resistant, β-Lactamase

1. Introduction

Increasing numbers of reports have documented the emergence of Escherichia coli capable of producing broad-spectrum β-lactamases. This is significant since the β-lactam antimicrobials are of therapeutic importance in humans and many domestic animals. Furthermore, many isolates are resistant to additional antimicrobial classes and are therefore multidrug-resistant (MDR).

Carriage of ESBL- and AmpC-producing E. coli has been documented in many species (Bortolaia et al., 2011). Antimicrobial use has been reported as a risk factor (Damborg et al., 2011, 2012; Maddox et al., 2012) and there is also evidence of sharing of organisms between species living in close proximity to each other (Dolejska et al., 2011). Considering the physical closeness in which many humans live with their pet companions, sharing of these organisms between humans and pets could pose a significant mutual risk.

Clinical disease associated with these organisms is well documented in humans. Initially the pattern was of hospital-acquired infection but community-acquired infection has become increasingly important. ESBL E. coli are associated with a variety of clinical diseases, in particular urinary tract infections, neonatal septicaemia and wound infections (Ben-Ami et al., 2009; Pitout, 2010). Reports of similar clinical disease associated with AmpC-producing E. coli are far fewer than those pertaining to ESBLs, however these also appear to be an emerging problem (Oteo et al., 2010).

Although most animal studies have focused on the zoonotic risk posed by carriage, there are increasing reports demonstrating the involvement of these organisms in clinical disease in domestic species in a variety of locations including Europe, North America, Asia and Australia. For example, in the United States AmpC (CMY-2) and ESBL-producing E. coli (O’Keefe et al., 2010; Sanchez et al., 2002; Shaheen et al., 2011) have been reported from canine clinical isolates. Clinical disease associated with AmpC-producing E. coli in dogs in Australia, was first reported in 2006 (Sidjabat et al., 2006). More recently, a survey of clinical isolates from dogs and horses in the Netherlands demonstrated a 2% prevalence of ESBL and AmpC-producing isolates (Dierikx et al., 2012).

The aim of this study was to evaluate the association of ESBL or AmpC production with multidrug-resistant E. coli isolated from clinical cases of urinary tract infection in dogs from a local patient population, over a period of time ranging from 2002 to 2011. Isolates were further characterized in terms of phylogenetic grouping, sequence type and virulence genotype. Plasmid replicon typing was also performed to identify the type and diversity of plasmids involved. Comparisons were made to a group of susceptible E. coli isolates, also associated with canine urinary tract infection and collected over a similar time frame.

2. Materials and methods

2.1. Source of clinical isolates

All 15 susceptible and 17/18 MDR isolates were identified in clinical cases seen at the Hospital for Small Animals, University of Edinburgh. One MDR isolate (R3) came from a local practice serviced by the University of Edinburgh's diagnostic microbiology service.

2.2. E. coli identification

A total of 33 clinical isolates from canine urinary tract infections were cultured on Blood and MacConkey agar. Any lactose fermenting colonies were confirmed as E. coli utilizing biochemical testing (API 10S® strip bioMerieux).

2.3. Antimicrobial susceptibility testing

Susceptibility testing was performed using the disc diffusion method in accordance with Clinical Laboratory Standards Institute (CLSI) guidelines. The following discs were used: Co-trimoxazole (25 μg); ciprofloxacin (1 μg); amoxicillin clavulanate (30 μg); cephalexin (30 μg); gentamicin (10 μg); tetracycline (10 μg); cefotaxime (30 μg). All discs were sourced from Mast group Ltd.

2.4. Control strains

The following control strains were utilized in both the phenotypic combination disc testing and for polymerase chain reaction. ATCC 25922™ (negative control); ATCC BA-199™ (SHV-3 positive control); NCTC 13353™ (CTX-M-15 positive control), and NCTC 13351™ (TEM-3 positive control).

2.5. Combination disc method for plasmid-mediated AmpC and ESBL detection

A commercially available AmpC and ESBL detection set (Mast group Ltd) was utilized. This comprised a set of 4 discs containing cefpodoxime plus or minus AmpC and ESBL inhibitors. Interpretation was made following the manufacturer's instructions.

2.6. PCR for ESBL and AmpC gene detection

DNA from single colonies of each isolate was prepared using the lysis method as previously described (Pérez-Pérez and Hanson, 2002). Primers for the genes blaTEM, blaSHV, blaCTX, blaCMY-1 group, blaCMY-2 group, blaOXA-1 group and blaOXA-2 group were derived from a previously established assay (Hasman et al., 2005). For additional detection of AmpC β-lactamase genes, multiplex PCR was performed on all samples using the methodology and primers previously described (Pérez-Pérez and Hanson, 2002). PCR products were electrophoresed in a 1% agarose gel, gel bands were excised, and sequences were compared to the NCBI database to confirm identity.

2.7. Multiplex PCR phylogenetic grouping of clinical isolates

DNA from single colonies was prepared using the Qiagen DNeasy blood and tissue extraction kit according to the manufacturer's instructions (Qiagen, UK). Multiplex PCR methodology was employed to assign the clinical isolates to one of four phylogenetic groups (A, B1, B2 or D). Primers and methodology have been described previously (Doumith et al., 2012).

2.8. PCR-based plasmid replicon typing of clinical isolates

DNA was isolated as described above for the phylogenetic grouping. Methodology involved the use of 8 multiplex reactions in a commercial kit (Diatheva, Italy) based on methodology described previously (Carattoli et al., 2005).

2.9. Identibac® microarray analysis

A microarray assay developed and carried out by the Animal Health Veterinary Laboratories Agency (AHVLA) was used (Batchelor et al., 2008). The microarray contained a selection of oligonucleotide probes mapping to a range of resistance and virulence-associated genes. Probe hybridizations resulting in signal intensities greater than 0.4 were considered positive indicating the presence of the gene.

2.10. MLST methodology

DNA was extracted using a DNeasy extraction kit (Qiagen) and performed as to the manufacturer's instructions. Sequencing of the DNA was carried out on an Illumina MiSeq (ARK Genomics). Raw sequence reads were aligned to two reference sequences, E. coli ABU83972 and E. coli MG1655 (Accession numbers NC_017631.1 and NC_000913.2), using BWA and Samtools (Li and Durbin, 2009). Sequence type calling (for multi-locus sequence typing) was performed using SRST (Inouye et al., 2012). Isolates which could not be typed using SRST were called manually using sequences mapped to MG1655. Sequences aligning to the MLST genes in MG1655 were extracted using VCFtools (Danecek et al., 2011) and Extractseq, (Rice, 2000) and entered manually into the MLST Database, hosted by University College Cork (http://mlst.ucc.ie/mlst/dbs/Ecoli).

2.11. Statistical methods

Comparisons were tested using Fisher's exact test. The criterion for statistical significance was taken to be P < 0.05.

3. Results

3.1. Culture and sensitivity

Between 2002 and 2011, 18 E. coli isolates associated with urinary tract infections (UTIs) in 16 dogs were identified as multidrug-resistant (MDR). Results of culture and sensitivity testing for the MDR isolates (R1-16) are displayed in Table 1. Two of the dogs had recurrent UTI infection. One case recurred 1 month later (R16a and b) and the second case 6 months later (R11a and b). The criterion used to make the MDR determination was resistance to 3 or more classes of antimicrobial on routine culture and sensitivity testing. All MDR isolates were resistant to amoxicillin clavulanate and tetracycline, 83% (15/18) were resistant to cephalexin, 78% (14/18) were resistant to co-trimoxazole, 56% (10/18) were resistant to ciprofloxacin, 22% (4/18) were resistant to gentamicin and 67% (12/18) were resistant to the 3rd generation cephalosporin cefotaxime. The latter was used as an indicator of broad-spectrum β-lactamase production.

Table 1.

Summary of phenotypic and genotypic characterization of the MDR E. coli isolates.

Isolate Isolation date (month/year) Resistance pattern 3rd Gen AmpC Pheno pAmpC PCR Phylo ST Replicon type
R1 9/2006 AMC CEF COT CIP TET R + + A 10 FII I1
R2 1/2008 AMC CEF COT TET R + + n/t 46 FII I1
R3 9/2010 AMC CEF COT CIP TET R + + A 744 FII I1
R4 10/2010 AMC CEF TET R + + D 648 FII I1
R5 3/2010 AMC CEF TET R + + D 963 FII I1
R6 2/2011 AMC CEF COT TET R + + D N FII I1
R7 12/2007 AMC CEF COT CIP TET R + + B1 539 B/O I1
R8 9/2011 AMC CEF COT CIP TET GEN S + − B1 23 FII FIB
R9 9/2011 AMC CEF COT TET R + + B1 101 I1
R10 3/2002 AMC CEF COT CIP TET R + + B2 167 FII FIA I1
R11a 2/2010 AMC CEF COT TET R + − D 10 −
R11b 8/2010 AMC CEF TET R + − D 372 −
R12 7/2006 AMC CEF COT TET GEN S − n/d D 372 I2
R13 8/2009 AMC COT CIP TET S − n/d A 10 FII FIA
R14 8/2008 AMC COT CIP TET S − n/d A 10 −
R15 4/2009 AMC CEF COT CIP TET S − n/d A 998 −
R16a 4/2011 AMC CEF CIP TET GEN R − n/d B1 23 FII B/O
R16b 5/2011 AMC COT CIP TET GEN S − n/d B2 23 FII FIB
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Resistance pattern identifies antimicrobials to which organisms were resistant: AMC = Amoxicillin clavulanate; CEF = Cephalexin; COT = co-trimoxazole; CIP = ciprofloxacin; TET = tetracycline; GEN = gentamicin. 3rd Gen identifies which organisms were resistant (R) or sensitive (S) to the 3rd generation cephalosporin cefotaxime. AmpC Pheno identifies which isolates were positive or negative for AmpC on the 4 disc phenotypic test. AmpC PCR identifies those isolates where plasmid associated AmpC genes could be identified by PCR (n/d designates not done), Phylo represents phylogenetic group, ST represents sequence type and N represents a novel ST type.

For comparison, 15 E. coli isolates were selected for study, based on the criteria that they were susceptible to all 7 antimicrobial classes listed above. These isolates were associated with urinary tract infections in dogs.

3.2. AmpC β-lactamase phenotype and genotype

To identify the contribution of either ESBL or AmpC β-lactamases to the MDR phenotype, all isolates (both MDR and susceptible groups) were tested using the 4 disc test as described in materials and methods. All susceptible isolates were negative (data not shown). A total of 67% (12/18) of MDR isolates were positive for AmpC production. ESBL production was not detected.

Using ampC multiplex PCR, 9/12 isolates phenotypically AmpC positive were genotypically positive for pAmpC (CITM group). Further simplex PCR identified 8 of these to be specifically associated with the CMY-2 gene. Identibac® microarray also detected a CMY gene signal in 8/9 isolates tested (isolates relate to R1–R7, R9 and R10 in Table 1).

3.3. Phylogenetic group and ST designation

PCR-based phylogenetic grouping was performed to assign the isolates to one of 4 phylogenetic groups, namely A, B1, B2 or D. Phylotypes A and B1 are considered to be associated with commensal status or intestinal pathotypes, while B2 and D are more commonly associated with strains causing extraintestinal infections (Tenaillon et al., 2010). Among the susceptible isolates B2 was the predominant phylogenetic group (10 isolates, 67%) with no isolates in group A, 2 isolates (13%) in the B1 grouping and 3 isolates (20%) in group D. In contrast, the MDR group showed a more even distribution among all 4 phylogenetic groups (1 isolate could not be typed), with B2 comprising the smallest category. The distribution for A, B1, B2 or D was 28%, 22%, 11% and 33% respectively. The proportion of MDR isolates identified as phylotype B2 differed significantly from the proportion of susceptible isolates identified as B2 (P < 0.001).

MLST identified 18 ST types among the E. coli isolates. Within the MDR group these were: ST10 (n = 4); ST 23 (n = 3); ST372 (n = 2); ST46 (n = 1); ST744 (n = 1); ST648 (n = 1); ST963 (n = 1); ST539 (n = 1); ST101 (n = 1); ST167 (n = 1); and ST 998 (n = 1). The two MDR isolates belonging to phylogenetic group B2 belonged to ST types 167 and 23. The overall association of ST type and phylogenetic group is listed in Table 1. Within the susceptible group ST types identified were: ST73 (n = 4); ST12 (n = 2); ST641 (n = 1); ST 127 (n = 1); ST10 (n = 1); ST625 (n = 1); ST929 (n = 1); and ST3005 (n = 1). A total of 3 isolates from the susceptible group and 1 from the MDR group did not map to existing ST types. None of the isolates belonged to ST131.

3.4. Identibac® microarray analysis

A panel of 11 specific probes was extracted from a much larger panel. These represent probes for which any isolate, either susceptible or MDR, demonstrated a positive result. The full list of probes against which isolates were tested can be found in the supplementary materials.

The virulence marker panel results are summarized in Table 3. Although the number of isolates examined was limited, there were significantly higher (P < 0.05) levels of carriage demonstrated for 7/11 specific virulence markers in the susceptible group compared to the MDR group.

Table 3.

Identification of virulence markers in the MDR and susceptible E. coli isolates. X indicates presence of the gene.

Isolate Phyl ireA iroN iss mchB mchC mchF mcm perA prfB senB sfaS
a a a a a a a
R1 A
R2 n/t
R3 A X X
R4 D X
R5 D
R6 D
R7 B1
R8 B1 X X X X X X
R9 B1 X X X X X
R10 B2
R11a D
R11b D
R12 D X
R13 A X X
R14 A
R15 A
R16a B1
R16b B2 X X X X X
S1 B2 X X X X X X X
S2 B2 X X X X X X X X
S3 B2 X X X X X X X X
S4 B2 X X X X X X X
S5 B2 X X X X X X X X
S6 B1 X X X X X X
S7 B2 X X X X X X
S8 D X X X X X
S9 B2 X X X X X X
S10 B2 X X X X X X X X X X X
S11 B2
S12 D X X X X
S13 D X X X X X
S14 B2 X X X X X X X X X
S15 B1
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R = MDR isolate; S = susceptible isolate; Phyl = phylogroup; ireA = siderophore receptor; iroN = enterobactin siderophore receptor; iss = increased serum survival; mchB = microcin H47 part of colicin H; mchC = MchC protein; mchF = ABC transporter protein; mcm = microcin M part of colicin H; perA = EPEC adherence factor; prfB = P-related fimbriae regulatory gene; senB = plasmid encoded enterotoxin; sfaS = S fimbriae minor subunit.

a

Identifies significant difference for MDR versus susceptible.

3.5. Plasmid replicon typing

In the susceptible group (Table 2) plasmid replicons could not be identified in 5 of the isolates. In the remaining 10 isolates, 6 isolates had 2 or more replicons and 4 isolates carried single replicons. The FII replicon was present in 5 isolates and the FIB replicon was present in 4 isolates. Overall 8 different replicon types were identified in this group. In the MDR group (Table 1), plasmid replicons could not be identified in 4 of the isolates. A total of 12 of the remaining 14 carried 2 or more replicons and only 2 isolates carried single replicons. The FII replicon was present in 11 isolates and the I1 replicon was present in 9 isolates. A total of 6 isolates carried the FII and I1 replicons together (these 6 isolates were all phenotypically and genotypically positive for pAmpC). Overall 6 different replicon types were identified in this group.

Table 2.

Summary of phylogenetic group and replicon type of the susceptible urinary tract isolates.

Isolate Isolation date (month/year) Phylo ST Replicon type
S1 1/2003 B2 73 –
S2 10/2011 B2 73 –
S3 11/2001 B2 12 –
S4 11/2001 B2 73 –
S5 11/2001 B2 N –
S6 1/2001 B1 641 X1
S7 10/2002 B2 12 I2 R
S8 1/2003 D N FIA
S9 1/2003 B2 127 FIB
S10 12/2011 B2 10 FII
S11 12/2000 B2 73 FII FIB
S12 10/2011 D N FII FIB
S13 1/2002 D 625 FII FIB B/O
S14 8/2001 B2 929 FII I1
S15 9/2001 B1 3005 B/O I1
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Phylo represents phylogenetic group, ST represents sequence type and N represents novel sequence types.

4. Discussion

These findings demonstrate clinically significant MDR E. coli in canine urinary tract infections. The antibiogram phenotype of isolates (Table 1.) shows that treatment options are limited. All MDR isolates were resistant to the recommended first line treatment amoxicillin clavulanate and more than half of the isolates were resistant to fluoroquinolones, a third line option (Weese et al., 2011).

MDR strains were analyzed in some detail. Comparisons were made to a susceptible group of canine UTI isolates from the same locality and indirectly to significant human clonal lineages. The latter is particularly important in light of concerns regarding the transfer of organisms between humans and domestic animals, and the potential for either to act as a reservoir of infection for the other.

AmpC rather than ESBL-producing E. coli were commonly identified among the MDR isolates. This is interesting because, in human UTIs associated with MDR E. coli, ESBLs (particularly the CTX-M group) seem to be the predominant enzymes responsible for broad-spectrum resistance to β-lactams. Although this study has a low number of isolates, other studies have also identified the presence of AmpC producing E. coli in dogs associated with both faecal carriage and clinical disease (Damborg et al., 2011; Dierikx et al., 2012; Murphy et al., 2009; Shaheen et al., 2011; Sidjabat et al., 2006; Tamang et al., 2012; Wedley et al., 2011). Furthermore, routine screening in our laboratory has continued to identify AmpC-producing isolates causing urinary tract infection (10 isolates from January 2012 to November 2013). Although this represents a relatively low local incidence, these cases are still clinically significant and therapeutically challenging.

The phylogenetic profile differed between the two groups of isolates. The susceptible group of UTI isolates predominantly belonged to the B2 phylogenetic group, as predicted from previous studies in humans and dogs (Johnson et al., 2003; Mao et al., 2012; Thompson et al., 2011). The MDR group meanwhile demonstrated a more even distribution across all four phylo-groups, with significantly less representation of the B2 phylo-group. It is worth noting that a more recent methodology has been able to assign E. coli to 8 rather than 4 phylo-groups (Clermont et al., 2013), which could have altered the profile of the isolates in this study, and future work should employ the revised methodology to provide greater detail and depth of characterization. In the context of the results from this study, isolates already assigned to B1 or B2 would be unlikely to change classification, so the difference between groups in the proportions of isolates in phylotype B2 should still be valid.

The virulence marker profile also differed between the two groups, suggesting a reduced virulence genotype in the MDR isolates compared to the susceptible ones. Previous studies have indicated that, in MDR isolates associated with UTI in both humans and dogs, there may be a shift away from the dominance of the B2 phylogenetic group and a decrease in certain virulence genes (Moreno et al., 2006; Vila et al., 2002). The reason for this pattern is unclear. Other researchers have speculated that less pathogenic phylogenetic groups are more receptive to the acquisition of the MDR phenotype (Johnson et al., 2004), or that acquisition of the MDR phenotype results in a trade off, with a loss of virulence traits. Whatever the order of events, it would seem logical that less pathogenic phylogenetic groups, with an MDR phenotype, would require certain conditions under which to cause clinical disease.

Sequence typing did not provide evidence for clonal spread of isolates in either group. Considering the extended sampling time this is probably not surprising. More pertinent perhaps was the fact that ST131 was not identified amongst any of our isolates. The O25b-ST131clonal lineage is one of the most important uropathogenic E. coli groups in humans. It belongs to the B2 phylogenetic group, is multidrug-resistant (almost always resistant to the fluoroquinolones and often resistant to 3rd generation cephalosporins), is often, but not always, an ESBL producer (CTXM-15) and is highly virulent (Oteo et al., 2010; Thompson et al., 2011). Total reports of ST131 in domestic animals are still extremely low and, although there is some support for interspecies transfer of ST131, it is unclear if animals are a major reservoir or incidental host of this ExPEC clonal lineage, or indeed if humans act as an animal reservoir (Platell et al., 2011). Certainly within the limitations of our study we found no evidence for this.

One MDR isolate (R4) was typed as ST648 phylogenetic group D. Strains of this clonal lineage that carry ESBLs, have been associated with bacteraemias in human patients in the Netherlands and NDM carbapenamases in human patients in the United Kingdom and Pakistan (Tamang et al., 2012).

Plasmid replicon typing was performed in order to establish the range and diversity of plasmids amongst both the MDR and susceptible isolates. Most of the plasmid replicons correspond to incompatibility groups. In the susceptible group replicon types could not be assigned to 5 of the isolates. This is perhaps not surprising, as the assay is designed to detect resistance plasmids. In the MDR group there were 4 isolates that were not assigned replicon types. Interestingly 2 of these (R11a and b) were phenotypically AmpC-producing, but it was not possible to detect plasmid-associated genes. We speculate that AmpC production in these isolates could be attributed to chromosomal mutations in the ampC promoter. Further analysis of these isolates will be required to confirm this. In the remainder of the MDR isolates the FII and I1 replicon types were the most highly represented. We observed a cluster comprising 6 isolates (R1–R6 Table 1) all AmpC-producing and carrying Inc FII/IncI1 plasmids. Since IncFII and IncI1 plasmids are two of a number of plasmid types that are particularly successful in their ability to spread multidrug resistance (Carattoli, 2011), further characterization and comparison of the plasmids from these isolates would be of particular interest.

With the exception of one case for which we have no history, all cases caused by MDR E. coli had underlying disease involving suppression of the immune system (e.g. hyperadrenocorticism, cancer chemotherapy), an anatomical abnormality of the genitourinary tract (e.g. detrusor muscle atony, ectopic ureters), and/or a history of prior antimicrobial treatment. Clinical details are summarized in Table 4. This is not a surprising finding since it can be envisaged that such factors will increase the potential of isolates, which we speculate may be less virulent, to cause clinical disease. What will be of interest is to follow the natural history of infections caused by these organisms in companion animals. It is highly probable that future changes in the epidemiology of MDR E. coli infections in dogs, will reflect those seen in the human population, where there has been a shift from hospital-acquired infection, analogous to what we have observed in this study in dogs, to a community-acquired infection, where they are associated with uncomplicated urinary tract disease.

Table 4.

Clinical summary of the cases from which MDR E. coli were isolated.

Isolate Breed Age (years) Summary of clinical details
R1 Dachshund 10 Surgery to treat intervertebral disc prolapse. Amoxicillin/clavulanate used for surgical prophylaxis.
R2 Weimeraner 9 Prostatic abscess. Surgical drainage and antibiotics (enrofloxacin and clindamycin).
R4 Cocker Spaniel 4 Immune mediated haemolytic anaemia, which was treated with immunosuppressant drugs.
R5 Cross breed 11 Hyperadrenocorticism. Amoxicillin/clavulanate administered for several months to manage concurrent liver disease.
R6 Boxer 9 Multiple mast cell tumours. Resection followed by cancer chemotherapy.
R7 Schnauzer 6 Mast cell tumour. Surgical management followed by cancer chemotherapy.
R8 Labrador 10 Surgery to resect intestinal adenocarcinoma. Post surgery developed pneumonia and treated with multiple antibiotics.
R9 Collie 10 Diabetes mellitus poorly controlled.
R10 German Shepherd 8 Prostatic infection and a perineal hernia accompanied by recurrent cystitis.
R11 Bouvier de Flandres 6 Placement of a gastrostomy tube. The stoma became infected requiring antimicrobials. Subsequently the dog developed recurrent urinary tract infections.
R12 Bearded Collie 1 Pyelonephritis. Initially treated with amoxicillin/clavulanate.
R13 Cocker Spaniel 7 Pancreatitis
R14 Golden Retriever 5 Ectopic ureters possibly acting as a predisposing factor for urinary tract infection.
R15 Yorkshire Terrier 11 Concurrent urolithiasis.
R16 Boxer cross 7 Detrusor muscle atony decreasing the ability to empty the bladder during urination.
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The main limitations of this study are the low number of MDR isolates, and the relatively extended time over which samples were collected. However, this reflects the relative scarcity of such MDR isolates associated with routine community-acquired canine urinary tract infections tested over the last decade at our laboratory, with the majority of the MDR isolates arising from more complex clinical cases at the small animal hospital.

5. Conclusion

MDR E. coli are a cause of a small but clinically significant number of urinary tract infections in dogs serviced by the veterinary microbiology service at Edinburgh University.

Broad-spectrum β-lactamase production is an important resistance mechanism and, unlike in human infections, AmpC rather than ESBL production predominates. This may reflect the gut carriage of AmpC E. coli in the dog. Amongst our isolates MDR E. coli are skewed towards less pathogenic phylogenetic groups and have a reduced virulence genotype. The variety of ST types does not support clonal spread; horizontal transfer of certain plasmid types may be a more important mechanism for the transmission of the MDR phenotype, judging by the frequency of the IncFII and IncI1 plasmid types. Most importantly, ST type analysis of both susceptible and MDR isolates suggests that dogs with urinary tract infection are not acting as a reservoir for zoonotic spread. Neither does it provide evidence for reverse zoonosis from the human population.

Conflict of interest

The authors have no financial or personal relationships with organizations or people that could inappropriately influence or bias their work

Acknowledgements

This work is jointly funded by Zoetis and the Biotechnology and Biological Sciences Research Council (BBSRC). This work has been submitted for publication with the approval of Zoetis. We would like to thank Ms Lorna Hume, Ms Jennifer Harris and Dr James Gibbons for technical assistance; the clinical staff at the Hospital for Small Animals, University of Edinburgh for allowing access to clinical material; the AHVLA for carrying out the Identibac® assay and Dr Darren Shaw for statistical advice.

Footnotes

☆

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

Appendix A. Supplementary data

The following are the supplementary data to this article:

mmc1.docx (72.2KB, docx)

References

  1. Batchelor M., Hopkins K.L., Liebana E., Slickers P., Ehricht R., Mafura M., Aarestrup F., Mevius D., Clifton-Hadley F.A., Woodward M.J., Davies R.H., Threlfall E.J., Anjum M.F. Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria. Int. J. Antimicrob. Agents. 2008;31:440–451. doi: 10.1016/j.ijantimicag.2007.11.017. [DOI] [PubMed] [Google Scholar]
  2. Ben-Ami R., Rodríguez-Baño J., Arslan H., Pitout J.D.D., Quentin C., Calbo E.S., Azap O.K., Arpin C., Pascual A., Livermore D.M., Garau J., Carmeli Y. A multinational survey of risk factors for infection with extended-spectrum beta-lactamase-producing enterobacteriaceae in nonhospitalized patients. Clin. Infect. Dis. 2009;49:682–690. doi: 10.1086/604713. [DOI] [PubMed] [Google Scholar]
  3. Bortolaia V., Larsen J., Damborg P., Guardabassi L. Potential pathogenicity and host range of extended-spectrum β-lactamase-producing Escherichia coli isolates from healthy poultry. Appl. Environ. Microb. 2011;77:5830–5833. doi: 10.1128/AEM.02890-10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carattoli A. Plasmids in Gram negatives: molecular typing of resistance plasmids. Int. J. Med. Microbiol. 2011;301:654–658. doi: 10.1016/j.ijmm.2011.09.003. [DOI] [PubMed] [Google Scholar]
  5. Carattoli A., Bertini A., Villa L., Falbo V., Hopkins K.L., Threlfall E.J. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods. 2005;63:219–228. doi: 10.1016/j.mimet.2005.03.018. [DOI] [PubMed] [Google Scholar]
  6. Clermont O., Christenson J.K., Denamur E., Gordon D.M. The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environ. Microbiol. Rep. 2013;5:58–65. doi: 10.1111/1758-2229.12019. [DOI] [PubMed] [Google Scholar]
  7. Damborg P., Gaustad I.B., Olsen J.E., Guardabassi L. Selection of CMY-2 producing Escherichia coli in the faecal flora of dogs treated with cephalexin. Vet. Microbiol. 2011;151:404–408. doi: 10.1016/j.vetmic.2011.03.015. [DOI] [PubMed] [Google Scholar]
  8. Damborg P., Marskar P., Baptiste K.E., Guardabassi L. Faecal shedding of CTX-M-producing Escherichia coli in horses receiving broad-spectrum antimicrobial prophylaxis after hospital admission. Vet. Microbiol. 2012;154:298–304. doi: 10.1016/j.vetmic.2011.07.005. [DOI] [PubMed] [Google Scholar]
  9. Danecek P., Auton A., Abecasis G., Albers C.A., Banks E., DePristo M.A., Handsaker R.E., Lunter G., Marth G.T., Sherry S.T., McVean G., Durbin R. The variant call format and VCFtools. Bioinformatics. 2011;27:2156–2158. doi: 10.1093/bioinformatics/btr330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dierikx C.M., van Duijkeren E., Schoormans A.H.W., van Essen-Zandbergen A., Veldman K., Kant A., Huijsdens X.W., van der Zwaluw K., Wagenaar J.A., Mevius D.J. Occurrence and characteristics of extended-spectrum-β-lactamase- and AmpC-producing clinical isolates derived from companion animals and horses. J. Antimicrob. Chemother. 2012;67:1–7. doi: 10.1093/jac/dks049. [DOI] [PubMed] [Google Scholar]
  11. Dolejska M., Duskova E., Rybarikova J., Janoszowska D., Roubalova E., Dibdakova K., Maceckova G., Kohoutova L., Literak I., Smola J., Cizek A. Plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre. J. Antimicrob. Chemother. 2011;66:757–764. doi: 10.1093/jac/dkq500. [DOI] [PubMed] [Google Scholar]
  12. Doumith M., Day M.J., Hope R., Wain J., Woodford N. Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups. J. Clin. Microbiol. 2012;50:3108–3110. doi: 10.1128/JCM.01468-12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hasman H., Mevius D., Veldman K., Olesen I., Aarestrup F.M. Beta-lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in the Netherlands. J. Antimicrob. Chemother. 2005;56:115–121. doi: 10.1093/jac/dki190. [DOI] [PubMed] [Google Scholar]
  14. Inouye M., Conway T.C., Zobel J., Holt K.E. Short read sequence typing (SRST): multi-locus sequence types from short reads. BMC Genomics. 2012;13:338. doi: 10.1186/1471-2164-13-338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Johnson J.R., Kaster N., Kuskowski M.A., Ling G.V. Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E. coli isolates from dogs with urinary tract infection. J. Clin. Microbiol. 2003;41:337–345. doi: 10.1128/JCM.41.1.337-345.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson J.R., Kuskowski M.A., Gajewski A., Sahm D.F., Karlowsky J.A. Virulence characteristics and phylogenetic background of multidrug-resistant and antimicrobial-susceptible clinical isolates of Escherichia coli from across the United States, 2000–2001. J. Infect. Dis. 2004;190:1739–1744. doi: 10.1086/425018. [DOI] [PubMed] [Google Scholar]
  17. Li H., Durbin R. Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics. 2009;25:1754–1760. doi: 10.1093/bioinformatics/btp324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Maddox T.W., Pinchbeck G.L., Clegg P.D., Wedley A.L., Dawson S., Williams N.J. Cross-sectional study of antimicrobial-resistant bacteria in horses. Part 2: Risk factors for faecal carriage of antimicrobial-resistant Escherichia coli in horses. Equine Vet. J. 2012;44:297–303. doi: 10.1111/j.2042-3306.2011.00440.x. [DOI] [PubMed] [Google Scholar]
  19. Mao B.-H., Chang Y.-F., Scaria J., Chang C.-C., Chou L.-W., Tien N., Wu J.-J., Tseng C.-C., Wang M.-C., Chang C.-C., Hsu Y.-M., Teng C.-H. Identification of Escherichia coli genes associated with urinary tract infections. J. Clin. Microbiol. 2012;50:449–456. doi: 10.1128/JCM.00640-11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moreno E., Prats G., Sabaté M., Pérez T., Johnson J.R., Andreu A. Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli. J. Antimicrob. Chemother. 2006;57:204–211. doi: 10.1093/jac/dki468. [DOI] [PubMed] [Google Scholar]
  21. Murphy C., Reid-Smith R.J., Prescott J.F., Bonnett B.N., Poppe C., Boerlin P., Weese J.S., Janecko N., McEwen S.A. Occurrence of antimicrobial resistant bacteria in healthy dogs and cats presented to private veterinary hospitals in southern Ontario: a preliminary study. Can. Vet. J. 2009;50:1047–1053. [PMC free article] [PubMed] [Google Scholar]
  22. O’Keefe A., Hutton T.A., Schifferli D.M., Rankin S.C. First detection of CTX-M and SHV extended-spectrum beta-lactamases in Escherichia coli urinary tract isolates from dogs and cats in the United States. Antimicrob. Agents Chemother. 2010;54:3489–3492. doi: 10.1128/AAC.01701-09. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oteo J., Cercenado E., Cuevas O., Bautista V., Delgado-Iribarren A., Orden B., Pérez-Vázquez M., García-Cobos S., Campos J. AmpC beta-lactamases in Escherichia coli: emergence of CMY-2-producing virulent phylogroup D isolates belonging mainly to STs 57, 115, 354, 393, and 420, and phylogroup B2 isolates belonging to the international clone O25b-ST131. Diagn. Microbiol. Infect. Dis. 2010;67:270–276. doi: 10.1016/j.diagmicrobio.2010.02.008. [DOI] [PubMed] [Google Scholar]
  24. Pérez-Pérez F.J., Hanson N.D. Detection of plasmid-mediated AmpCNL-lactamase genes in clinical isolates by using multiplex PCR. J. Clin. Microbiol. 2002;40:2153–2162. doi: 10.1128/JCM.40.6.2153-2162.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pitout J.D.D. Infections with extended-spectrum beta-lactamase-producing enterobacteriaceae: changing epidemiology and drug treatment choices. Drugs. 2010;70:313–333. doi: 10.2165/11533040-000000000-00000. [DOI] [PubMed] [Google Scholar]
  26. Platell J.L., Johnson J.R., Cobbold R.N., Trott D.J. Multi-drug resistant extraintestinal pathogenic Escherichia coli of sequence type ST 131 in animals and foods. Vet. Microbiol. 2011;153:99–108. doi: 10.1016/j.vetmic.2011.05.007. [DOI] [PubMed] [Google Scholar]
  27. Rice P. The European molecular biology open software suite EMBOSS: the European molecular biology open software suite. 2000;9525:2–3. doi: 10.1016/s0168-9525(00)02024-2. [DOI] [PubMed] [Google Scholar]
  28. Sanchez S., McCrakin Stevenson M.A., Hudson C.R., Maier M., Buffington T., Dam Q., Maurer J.J. Characterization of multidrug-resistant Escherichia coli isolates associated with nosocomial infections in dogs. J. Clin. Microbiol. 2002;40:3586–3595. doi: 10.1128/JCM.40.10.3586-3595.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shaheen B.W., Nayak R., Foley S.L., Kweon O., Deck J., Park M., Rafii F., Boothe D.M. Molecular characterization of resistance to extended-spectrum cephalosporins in clinical Escherichia coli isolates from companion animals in the United States. Antimicrob. Agents Chemother. 2011;55:5666–5675. doi: 10.1128/AAC.00656-11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sidjabat H.E., Townsend K.M., Hanson N.D., Bell J.M., Stokes H.W., Gobius K.S., Moss S.M., Trott D.J. Identification of bla(CMY-7) and associated plasmid-mediated resistance genes in multidrug-resistant Escherichia coli isolated from dogs at a veterinary teaching hospital in Australia. J. Antimicrob. Chemother. 2006;57:840–848. doi: 10.1093/jac/dkl057. [DOI] [PubMed] [Google Scholar]
  31. Tamang M.D., Nam H.-M., Jang G.-C., Kim S.-R., Chae M.H., Jung S.-C., Byun J.-W., Park Y.H., Lim S.-K. Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea. Antimicrob. Agents Chemother. 2012;56:2705–2712. doi: 10.1128/AAC.05598-11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tenaillon O., Skurnik D., Picard B., Denamur E. The population genetics of commensal Escherichia coli. Nat. Rev. Microbiol. 2010;8:207–217. doi: 10.1038/nrmicro2298. [DOI] [PubMed] [Google Scholar]
  33. Thompson M.F., Litster A.L., Platell J.L., Trott D.J. Canine bacterial urinary tract infections: new developments in old pathogens. Vet. J. 2011;190:22–27. doi: 10.1016/j.tvjl.2010.11.013. [DOI] [PubMed] [Google Scholar]
  34. Vila J., Simon K., Ruiz J., Horcajada J.P., Velasco M., Barranco M., Moreno A., Mensa J. Are quinolone-resistant uropathogenic Escherichia coli less virulent? J. Infect. Dis. 2002;186:1039–1042. doi: 10.1086/342955. [DOI] [PubMed] [Google Scholar]
  35. Wedley A.L., Maddox T.W., Westgarth C., Coyne K.P., Pinchbeck G.L., Williams N.J., Dawson S. Prevalence of antimicrobial-resistant Escherichia coli in dogs in a cross-sectional, community-based study. Vet. Rec. 2011;168:354. doi: 10.1136/vr.d1540. [DOI] [PubMed] [Google Scholar]
  36. Weese J.S., Blondeau J.M., Boothe D., Breitschwerdt E.B., Guardabassi L., Hillier A., Lloyd D.H., Papich M.G., Rankin S.C., Turnidge J.D., Sykes J.E. Antimicrobial use guidelines for treatment of urinary tract disease in dogs and cats: antimicrobial guidelines working group of the international society for companion animal infectious diseases. Vet. Med. Int. 2011;2011:263768. doi: 10.4061/2011/263768. [DOI] [PMC free article] [PubMed] [Google Scholar]

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