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. 1985 Jan;82(1):217–221. doi: 10.1073/pnas.82.1.217

Identification and partial characterization of a prolactin-like hormone produced by rat decidual tissue.

P G Jayatilak, L A Glaser, R Basuray, P A Kelly, G Gibori
PMCID: PMC397003  PMID: 2982145

Abstract

Previous studies have strongly, but indirectly, suggested that rat decidual tissue produces a prolactin-like hormone, decidual luteotropin, which markedly affects luteal cell function. However, it was also found that extracts of decidual tissue do not cross-react with antisera to either rat or ovine prolactin (PRL). The purpose of this study was to determine whether the decidual tissue contains a substance that binds to PRL receptors in rat luteal membranes and, if so, to identify, quantitate, and characterize this molecule with the use of an ovarian radioreceptor assay. Decidual tissue was induced in day 5 pseudopregnant rats by scratching the antimesometrial wall of the uterus; it was collected on day 9 and homogenized and extracted. Decidual tissue extracts bound specifically to ovarian PRL receptors. Graded dilutions of the extracts yielded curves that were parallel to the ovine PRL standard, indicating that decidual luteotropin competes for the same receptor sites on rat luteal membranes. To determine the levels of decidual luteotropin throughout pseudopregnancy, decidual tissue was obtained on each day between days 6-12. The PRL-like activity was detectable in decidual tissue as early as day 6, reached a maximum on day 9, and declined thereafter. The elution profile obtained from gel filtration of a day 9 decidual tissue extract displayed a major component of decidual luteotropin eluting at a Ve/Vo ratio of approximately equal to 2.0. Column chromatography indicated that decidual luteotropin corresponds to a protein with a molecular weight of 23,500. The hormone was heat labile, digestible by trypsin, and appears to contain disulfide linkages. In summary, this study reports the identification, quantitation, and partial characterization of a PRL-like hormone produced by the decidual tissue of the rat.

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Selected References

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  1. Anteby S. O., Bauminger S., Zor U., Lindner H. R. Prostaglandin synthesis in decidual tissue of the rate uterus. Prostaglandins. 1975 Dec;10(6):991–999. doi: 10.1016/s0090-6980(75)80046-3. [DOI] [PubMed] [Google Scholar]
  2. Basuray R., Gibori G. Luteotropic action of decidual tissue in the pregnant rat. Biol Reprod. 1980 Oct;23(3):507–512. doi: 10.1095/biolreprod23.3.507. [DOI] [PubMed] [Google Scholar]
  3. Basuray R., Jaffe R. C., Gibori G. Role of decidual luteotropin and prolactin in the control of luteal cell receptors of estradiol. Biol Reprod. 1983 Apr;28(3):551–556. doi: 10.1095/biolreprod28.3.551. [DOI] [PubMed] [Google Scholar]
  4. Bigazzi M., Nardi E., Bruni P., Petrucci F. Relaxin in human decidua. J Clin Endocrinol Metab. 1980 Oct;51(4):939–941. doi: 10.1210/jcem-51-4-939. [DOI] [PubMed] [Google Scholar]
  5. Castracane V. D., Rothchild I. Luteotrophic action of decidual tissue in the rat: comparison of jugular and uterine vein progesterone levels, and the effect of ligation of the utero-ovarian conncections. Biol Reprod. 1976 Nov;15(4):497–503. doi: 10.1095/biolreprod15.4.497. [DOI] [PubMed] [Google Scholar]
  6. Finn C. A. The biology of decidual cells. Adv Reprod Physiol. 1971;5:1–26. [PubMed] [Google Scholar]
  7. Gibori G., Basuray R., McReynolds B. Luteotropic role of the decidual tissue in the rat: dependency on intraluteal estradiol. Endocrinology. 1981 Jun;108(6):2060–2066. doi: 10.1210/endo-108-6-2060. [DOI] [PubMed] [Google Scholar]
  8. Gibori G., Richards J. S. Dissociation of two distinct luteotropic effects of prolactin: regulation of luteinizing hormone-receptor content and progesterone secretion during pregnancy. Endocrinology. 1978 Mar;102(3):767–774. doi: 10.1210/endo-102-3-767. [DOI] [PubMed] [Google Scholar]
  9. Gibori G., Richards J. S., Keyes P. L. Synergistic effects of prolactin and estradiol in the luteotropic process in the pregnant rat: regulation of estradiol receptor by prolactin. Biol Reprod. 1979 Sep;21(2):419–423. doi: 10.1095/biolreprod21.2.419. [DOI] [PubMed] [Google Scholar]
  10. Gibori G., Rothchild I., Pepe G. J., Morishige W. K., Lam P. Luteotrophic action of decidual tissue in the rat. Endocrinology. 1974 Oct;95(4):1113–1118. doi: 10.1210/endo-95-4-1113. [DOI] [PubMed] [Google Scholar]
  11. Glaser L. A., Kelly P. A., Gibori G. Differential action and secretion of rat placental lactogens. Endocrinology. 1984 Sep;115(3):969–976. doi: 10.1210/endo-115-3-969. [DOI] [PubMed] [Google Scholar]
  12. Golander A., Hurley T., Barrett J., Hizi A., Handwerger S. Prolactin synthesis by human chorion-decidual tissue: a possible source of prolactin in the amniotic fluid. Science. 1978 Oct 20;202(4365):311–313. doi: 10.1126/science.694535. [DOI] [PubMed] [Google Scholar]
  13. Herington A. C., Graham J., Healy D. L. The presence of lactogen receptors in human chorion laeve. J Clin Endocrinol Metab. 1980 Dec;51(6):1466–1468. doi: 10.1210/jcem-51-6-1466. [DOI] [PubMed] [Google Scholar]
  14. Kelly P. A., Shiu R. P., Robertson M. C., Friesen H. G. Characterization of rat chorionic mammotropin. Endocrinology. 1975 May;96(5):1187–1195. doi: 10.1210/endo-96-5-1187. [DOI] [PubMed] [Google Scholar]
  15. Lobel B. L., Tic L., Shelesnyak M. C. Studies on the mechanism of nidation. XVII. Histochemical analysis of decidualization in the rat. 4. Regression of deciduomata. Acta Endocrinol (Copenh) 1965 Dec;50(4):537–559. doi: 10.1530/acta.0.0500537. [DOI] [PubMed] [Google Scholar]
  16. Lobel B. L., Tic L., Shelesnyak M. C. Studies on the mechanism of nidation. XVII. Histochemical analysis of decidualization in the rat. I. Framework: oestrous cycle and pseudopregnancy. Acta Endocrinol (Copenh) 1965 Nov;50(3):452–468. doi: 10.1530/acta.0.0500452. [DOI] [PubMed] [Google Scholar]
  17. Maslar I. A., Riddick D. H. Prolactin production by human endometrium during the normal menstrual cycle. Am J Obstet Gynecol. 1979 Nov 15;135(6):751–754. doi: 10.1016/0002-9378(79)90386-7. [DOI] [PubMed] [Google Scholar]
  18. McCoshen J. A., Tomita K., Fernandez C., Tyson J. E. Specific cells of human amnion selectively localize prolactin. J Clin Endocrinol Metab. 1982 Jul;55(1):166–169. doi: 10.1210/jcem-55-1-166. [DOI] [PubMed] [Google Scholar]
  19. McWey L. A., Singhas C. A., Rogol A. D. Prolactin binding sites on human chorion-decidua tissue. Am J Obstet Gynecol. 1982 Oct 1;144(3):283–288. doi: 10.1016/0002-9378(82)90580-4. [DOI] [PubMed] [Google Scholar]
  20. Richards J. S., Williams J. J. Luteal cell receptor content for prolactin (PRL) and luteinizing hormone (LH): regulation by LH and PRL. Endocrinology. 1976 Dec;99(6):1571–1581. doi: 10.1210/endo-99-6-1571. [DOI] [PubMed] [Google Scholar]
  21. Riddick D. H., Kusmik W. F. Decidua: a possible source of amniotic fluid prolactin. Am J Obstet Gynecol. 1977 Jan 15;127(2):187–190. doi: 10.1016/s0002-9378(16)33248-3. [DOI] [PubMed] [Google Scholar]
  22. Riddick D. H., Luciano A. A., Kusmik W. F., Maslar I. A. De novo synthesis of prolactin by human decidua. Life Sci. 1978 Nov 9;23(19):1913–1921. doi: 10.1016/0024-3205(78)90557-x. [DOI] [PubMed] [Google Scholar]
  23. Rothchild I., Gibori G. The luteotrophic action of decidual tissue: the stimulating effect of decidualization on the serum progesterone level of pseudopregnant rats. Endocrinology. 1975 Oct;97(4):838–842. doi: 10.1210/endo-97-4-838. [DOI] [PubMed] [Google Scholar]
  24. Shiu R. P., Kelly P. A., Friesen H. G. Radioreceptor assay for prolactin and other lactogenic hormones. Science. 1973 Jun 1;180(4089):968–971. doi: 10.1126/science.180.4089.968. [DOI] [PubMed] [Google Scholar]
  25. Smith M. S., Neill J. D. Termination at midpregnancy of the two daily surges of plasma prolactin initiated by mating in the rat. Endocrinology. 1976 Mar;98(3):696–701. doi: 10.1210/endo-98-3-696. [DOI] [PubMed] [Google Scholar]
  26. Taga M., Satoh M., Minaguchi H., Mizuno M., Sakamoto S. Synthesis and secretion of prolactin in the decidualized human endometrium unassociated with pregnancy in vitro. Endocrinol Jpn. 1982 Jun;29(3):335–340. doi: 10.1507/endocrj1954.29.335. [DOI] [PubMed] [Google Scholar]
  27. Terranova P. F. Relationship between the decidual response and ovarian steroidogenesis in the unilaterally ovariectomized pseudopregnant rat. J Endocrinol. 1980 Jun;85(3):525–531. doi: 10.1677/joe.0.0850525. [DOI] [PubMed] [Google Scholar]
  28. Terranova P. F. Relationships among progesterone, androgen, and estradiol in corpora lutea and nonluteal ovarian compartments of pseudopregnant rats: effects of decidual tissue. Endocrinology. 1980 Mar;106(3):758–763. doi: 10.1210/endo-106-3-758. [DOI] [PubMed] [Google Scholar]
  29. Thorell J. I., Johansson B. G. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta. 1971 Dec 28;251(3):363–369. doi: 10.1016/0005-2795(71)90123-1. [DOI] [PubMed] [Google Scholar]
  30. Tomita K., McCoshen J. A., Friesen H. G., Tyson J. E. Quantitative comparison between biological and immunological activities of prolactin derived from human fetal and maternal sources. J Clin Endocrinol Metab. 1982 Aug;55(2):269–271. doi: 10.1210/jcem-55-2-269. [DOI] [PubMed] [Google Scholar]
  31. Tonkowicz P., Robertson M., Voogt J. Secretion of rat placental lactogen by the fetal placenta and its inhibitory effect on prolactin surges. Biol Reprod. 1983 Apr;28(3):707–716. doi: 10.1095/biolreprod28.3.707. [DOI] [PubMed] [Google Scholar]
  32. Williams G. H., Hammond J. M., Weisz J., Mortel R. Binding sites for lactogenic hormone in the rat uterus. Biol Reprod. 1978 May;18(4):697–706. doi: 10.1095/biolreprod18.4.697. [DOI] [PubMed] [Google Scholar]
  33. Yogev L., Terkel J. The temporal relationship between implantation and termination of nocturnal prolactin surges in pregnant lactating rats. Endocrinology. 1978 Jan;102(1):160–165. doi: 10.1210/endo-102-1-160. [DOI] [PubMed] [Google Scholar]

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