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. 2014 Mar 4;6(3):1037–1048. doi: 10.3390/v6031037

Figure 3.

Figure 3

Detection of hepatitis A virus (HAV) using soybean agglutinin-linked magnetic bead separation (SMS) with RT-PCR. (A) Determination of the detection limit of HAV using RT-PCR and 10-fold serial dilutions of a HAV stock. M, 100 bp marker; N, negative; (B) Determination of the binding of 10-fold serial dilutions (10−1–10−5) of HAV stock to soybean agglutinin (SBA); (C) Detection of HAV using SMS combined with RT-PCR. The limit of detection for the RT-PCR assay was assessed using 10-fold serial dilutions (10−1–10−4) of HAV (267 bp) present in the washout and eluent fractions. M, 100 bp marker; N, negative.