Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 28;59(4):1351–1365. doi: 10.1002/hep.26938

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Hepatology published by Wiley on behalf of the American Association for the Study of Liver Diseases

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Fig 4 — In vitro CD8⁺ T_reg cell suppression is dictated by the Tim-3 inhibitory pathway. C57BL/6 mice were IV infected with 2.5 × 10⁷ IU of Ad-Ova, and (A) bulk CD8⁺ T_reg cells were isolated from day 7 IV livers. Before coculture with CFSE-labeled naïve Thy1.1⁺CD8⁺ OT-I T cells at a 1:1 ratio and SIINKFEKL-pulsed BMDCs, CD8⁺ T_reg cells were left alone or precoated with anti-PD-L1 Ab, anti-Tim-3 Ab, or both. Cell cultures were analyzed at day 3 for CFSE dilution. (B) The number of dividing OT-I T cells, OT-I T-cell division index, and percent suppression displayed by IV CD8⁺ T_reg cells was calculated at days 2 and 3 of culture. (C) Concentrations of IL-2 and IFN-γ were measured in the day 3 culture supernatants by ELISA (one-way ANOVA/Tukey's posttest; n = 6-9 per group). Numbers in histograms represent percentages (mean ± standard error of the mean). *P < 0.05; **P < 0.01; ***P < 0.001.