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. 1985 Jan;82(2):589–593. doi: 10.1073/pnas.82.2.589

Electroconvulsive shock increases preproenkephalin messenger RNA abundance in rat hypothalamus.

K Yoshikawa, J S Hong, S L Sabol
PMCID: PMC397086  PMID: 2578669

Abstract

Daily administration of electroconvulsive shock (ECS) to rats for 10 days increased the content of [Met5]enkephalin in the hypothalamus and the striatum by 64% and 45%, respectively. The effect of ECS on the relative abundance of mRNA coding for the enkephalin precursor preproenkephalin was investigated. Analysis by cell-free translation of polyadenylylated RNA and immunoprecipitation of preproenkephalin revealed ECS-elicited increases of 79% and 14% in preproenkephalin mRNA activity in the hypothalamus and striatum, respectively. ECS treatment did not affect the general translational activity of total polyadenylylated RNA from these brain regions. A 32P-labeled probe prepared from a rat preproenkephalin cDNA clone hybridized with an apparently single species of polyadenylylated RNA of approximately equal to 1450 nucleotides from both hypothalamus and striatum. Dot-blot hybridization of polyadenylylated RNA with the rat probe indicated that ECS elicits a 76% increase in the preproenkephalin mRNA abundance in the hypothalamus and no significant change in the striatum. These results suggest that ECS treatment leads to enhanced biosynthesis of the enkephalin precursor in hypothalamic neurons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergstrom D. A., Kellar K. J. Effect of electroconvulsive shock on monoaminergic receptor binding sites in rat brain. Nature. 1979 Mar 29;278(5703):464–466. doi: 10.1038/278464a0. [DOI] [PubMed] [Google Scholar]
  2. Carrasco M. A., Dias R. D., Izquierdo I. Naloxone reverses retrograde amnesia induced by electroconvulsive shock. Behav Neural Biol. 1982 Apr;34(4):352–357. doi: 10.1016/s0163-1047(82)91738-1. [DOI] [PubMed] [Google Scholar]
  3. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  4. Comb M., Rosen H., Seeburg P., Adelman J., Herbert E. Primary structure of the human proenkephalin gene. DNA. 1983;2(3):213–229. doi: 10.1089/dna.1983.2.213. [DOI] [PubMed] [Google Scholar]
  5. Comb M., Seeburg P. H., Adelman J., Eiden L., Herbert E. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA. Nature. 1982 Feb 25;295(5851):663–666. doi: 10.1038/295663a0. [DOI] [PubMed] [Google Scholar]
  6. Dandekar S., Sabol S. L. Cell-free translation and partial characterization of proenkephalin messenger RNA from bovine striation. Biochem Biophys Res Commun. 1982 Mar 15;105(1):67–74. doi: 10.1016/s0006-291x(82)80011-9. [DOI] [PubMed] [Google Scholar]
  7. Glowinski J., Iversen L. L. Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain. J Neurochem. 1966 Aug;13(8):655–669. doi: 10.1111/j.1471-4159.1966.tb09873.x. [DOI] [PubMed] [Google Scholar]
  8. Gubler U., Seeburg P., Hoffman B. J., Gage L. P., Udenfriend S. Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides. Nature. 1982 Jan 21;295(5846):206–208. doi: 10.1038/295206a0. [DOI] [PubMed] [Google Scholar]
  9. Hong J. S., Gillin J. C., Yang H. Y., Costa E. Repeated electroconvulsive shocks and the brain content of endorphins. Brain Res. 1979 Nov 16;177(2):273–278. doi: 10.1016/0006-8993(79)90778-9. [DOI] [PubMed] [Google Scholar]
  10. Kety S. S., Javoy F., Thierry A. M., Julou L., Glowinski J. A sustained effect of electroconvulsive shock on the turnover of norepinephrine in the central nervous system of the rat. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1249–1254. doi: 10.1073/pnas.58.3.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Land H., Grez M., Hauser H., Lindenmaier W., Schütz G. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 1981 May 25;9(10):2251–2266. doi: 10.1093/nar/9.10.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Legon S., Glover D. M., Hughes J., Lowry P. J., Rigby P. W., Watson C. J. The structure and expression of the preproenkephalin gene. Nucleic Acids Res. 1982 Dec 20;10(24):7905–7918. doi: 10.1093/nar/10.24.7905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lehrach H., Diamond D., Wozney J. M., Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. doi: 10.1021/bi00640a033. [DOI] [PubMed] [Google Scholar]
  15. Lewis J. W., Cannon J. T., Chudler E. H., Liebeskind J. C. Effects of naloxone and hypophysectomy on electroconvulsive shock-induced analgesia. Brain Res. 1981 Mar 9;208(1):230–233. doi: 10.1016/0006-8993(81)90639-9. [DOI] [PubMed] [Google Scholar]
  16. Masserano J. M., Takimoto G. S., Weiner N. Electroconvulsive shock increases tyrosine hydroxylase activity in the brain and adrenal gland of the rat. Science. 1981 Nov 6;214(4521):662–665. doi: 10.1126/science.6117127. [DOI] [PubMed] [Google Scholar]
  17. Musacchio J. M., Julou L., Kety S. S., Glowinski J. Increase in rat brain tyrosine hydroxylase activity produced by electroconvulsive shock. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1117–1119. doi: 10.1073/pnas.63.4.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Noda M., Furutani Y., Takahashi H., Toyosato M., Hirose T., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin. Nature. 1982 Jan 21;295(5846):202–206. doi: 10.1038/295202a0. [DOI] [PubMed] [Google Scholar]
  19. Sabol S. L., Liang C. M., Dandekar S., Kranzler L. S. In vitro biosynthesis and processing of immunologically identified methionine-enkephalin precursor protein. J Biol Chem. 1983 Feb 25;258(4):2697–2704. [PubMed] [Google Scholar]
  20. Sabol S. L., Yoshikawa K., Hong J. S. Regulation of methionine-enkephalin precursor messenger RNA in rat striatum by haloperidol and lithium. Biochem Biophys Res Commun. 1983 Jun 15;113(2):391–399. doi: 10.1016/0006-291x(83)91739-4. [DOI] [PubMed] [Google Scholar]
  21. Tang F., Costa E., Schwartz J. P. Increase of proenkephalin mRNA and enkephalin content of rat striatum after daily injection of haloperidol for 2 to 3 weeks. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3841–3844. doi: 10.1073/pnas.80.12.3841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tortella F. C., Cowan A., Belenky G. L., Holaday J. W. Opiate-like electroencephalographic and behavioral effects of electroconvulsive shock in rats. Eur J Pharmacol. 1981 Dec 3;76(2-3):121–128. doi: 10.1016/0014-2999(81)90493-3. [DOI] [PubMed] [Google Scholar]
  23. Urca G., Yitzhaky J., Frenk H. Different opioid systems may participate in post-electro-convulsive shock (ECS) analgesia and catalepsy. Brain Res. 1981 Aug 31;219(2):385–396. doi: 10.1016/0006-8993(81)90301-2. [DOI] [PubMed] [Google Scholar]
  24. Vanderhaeghen J. J., Lotstra F., Liston D. R., Rossier J. Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons. Proc Natl Acad Sci U S A. 1983 Aug;80(16):5139–5143. doi: 10.1073/pnas.80.16.5139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. White B. A., Bancroft F. C. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. J Biol Chem. 1982 Aug 10;257(15):8569–8572. [PubMed] [Google Scholar]
  26. Williams R. G., Dockray G. J. Distribution of enkephalin-related peptides in rat brain: immunohistochemical studies using antisera to met-enkephalin and met-enkephalin Arg6Phe7. Neuroscience. 1983 Jul;9(3):563–586. doi: 10.1016/0306-4522(83)90175-6. [DOI] [PubMed] [Google Scholar]
  27. Yoshikawa K., Hong J. S. The enkephalin system in the rat anterior pituitary: regulation by gonadal steroid hormones and psychotropic drugs. Endocrinology. 1983 Oct;113(4):1218–1227. doi: 10.1210/endo-113-4-1218. [DOI] [PubMed] [Google Scholar]
  28. Yoshikawa K., Williams C., Sabol S. L. Rat brain preproenkephalin mRNA. cDNA cloning, primary structure, and distribution in the central nervous system. J Biol Chem. 1984 Nov 25;259(22):14301–14308. [PubMed] [Google Scholar]

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