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. 1985 Feb;82(3):643–647. doi: 10.1073/pnas.82.3.643

Point mutation generates constitutive expression of an inducible eukaryotic gene.

R A Sumrada, T G Cooper
PMCID: PMC397101  PMID: 2983306

Abstract

We describe the analysis of two cis-dominant mutations that result in constitutive expression of the inducible CAR1 gene from yeast. One mutation results from insertion of a Ty element just upstream from the point where CAR1-specific transcription begins. The other mutation is a C-to-G transversion at position -153. Isolation of this point mutation, outside of the transcribed region of CAR1, suggests that expression of this gene is regulated at transcription. It also demonstrates the feasibility and usefulness of analyzing the regulatory sequences of eukaryotic genes on a nucleotide-by-nucleotide basis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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