Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Feb;82(3):703–707. doi: 10.1073/pnas.82.3.703

Expression of human adenosine deaminase using a transmissable murine retrovirus vector system.

R L Friedman
PMCID: PMC397114  PMID: 3856223

Abstract

Human adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) was expressed at high levels in cultured mouse cells using a transmissable murine retrovirus vector system. A cDNA clone encoding ADA has been inserted into a plasmid vector containing retroviral transcription and packaging signals as well as a selectable gene for G418 resistance. The constructions were transfected into psi 2 cells, which package the recombinant retroviral genomes into replication-defective virus particles. Isoenzyme analysis for ADA in G418-selected psi 2 cells showed at least 20-fold more human ADA activity than endogenous mouse ADA activity. A mouse T-cell lymphoma line, BL/VL3, was cocultured with transformed psi 2 cells producing human ADA, and some of the cocultured cells were selected for resistance to G418. Both G418-selected and unselected cocultured cells expressed human ADA activity at 25%-50% the level of the endogenous enzyme. Thus, efficient retroviral transduction of ADA expression was obtained.

Full text

PDF
703

Images in this article

705Image
on p.705
705Image
on p.705
706Image
on p.706
706Image
on p.706

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackeret C., Llüss H. J., Hitzig W. H. Hereditary sever combined immunodeficiency and adenosine deaminase deficiency. Pediatr Res. 1976 Jan;10(1):67–70. doi: 10.1203/00006450-197601000-00013. [DOI] [PubMed] [Google Scholar]
  2. Adams A., Harkness R. A. Adenosine deaminase activity in thymus and other human tissues. Clin Exp Immunol. 1976 Dec;26(3):647–649. [PMC free article] [PubMed] [Google Scholar]
  3. Adrian G. S., Wiginton D. A., Hutton J. J. Structure of adenosine deaminase mRNAs from normal and adenosine deaminase-deficient human cell lines. Mol Cell Biol. 1984 Sep;4(9):1712–1717. doi: 10.1128/mcb.4.9.1712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  5. Cepko C. L., Roberts B. E., Mulligan R. C. Construction and applications of a highly transmissible murine retrovirus shuttle vector. Cell. 1984 Jul;37(3):1053–1062. doi: 10.1016/0092-8674(84)90440-9. [DOI] [PubMed] [Google Scholar]
  6. Chechik B. E., Jason J., Shore A., Baker M., Dosch H. M., Gelfand E. W. Quantitation of human thymus/leukemia-associated antigen by radioimmunoassay in different forms of leukemia. Blood. 1979 Dec;54(6):1400–1406. [PubMed] [Google Scholar]
  7. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  8. Coleman M. S., Greenwood M. F., Hutton J. J., Holland P., Lampkin B., Krill C., Kastelic J. E. Adenosine deaminase, terminal deoxynucleotidyl transferase (TdT), and cell surface markers in childhood acute leukemia. Blood. 1978 Dec;52(6):1125–1131. [PubMed] [Google Scholar]
  9. Cone R. D., Mulligan R. C. High-efficiency gene transfer into mammalian cells: generation of helper-free recombinant retrovirus with broad mammalian host range. Proc Natl Acad Sci U S A. 1984 Oct;81(20):6349–6353. doi: 10.1073/pnas.81.20.6349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friedrich W., Goldmann S. F., Vetter U., Fliedner T. M., Heymer B., Peter H. H., Reisner Y., Kleihauer E. Immunoreconstitution in severe combined immunodeficiency after transplantation of HLA-haploidentical, T-cell-depleted bone marrow. Lancet. 1984 Apr 7;1(8380):761–764. doi: 10.1016/s0140-6736(84)91277-7. [DOI] [PubMed] [Google Scholar]
  11. Gelfand E. W., Dosch H. M. Differentiation of precursor T lymphocytes in man and delineation of the selective abnormalities in severe combined immune deficiency disease. Clin Immunol Immunopathol. 1982 Dec;25(3):303–315. doi: 10.1016/0090-1229(82)90195-7. [DOI] [PubMed] [Google Scholar]
  12. Giblett E. R., Anderson J. E., Cohen F., Pollara B., Meuwissen H. J. Adenosine-deaminase deficiency in two patients with severely impaired cellular immunity. Lancet. 1972 Nov 18;2(7786):1067–1069. doi: 10.1016/s0140-6736(72)92345-8. [DOI] [PubMed] [Google Scholar]
  13. Hellerman J. G., Cone R. C., Potts J. T., Jr, Rich A., Mulligan R. C., Kronenberg H. M. Secretion of human parathyroid hormone from rat pituitary cells infected with a recombinant retrovirus encoding preproparathyroid hormone. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5340–5344. doi: 10.1073/pnas.81.17.5340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hirschhorn R., Vawter G. F., Kirkpatrick J. A., Jr, Rosen F. S. Adenosine deaminase deficiency: frequency and comparative pathology in autosomally recessive severe combined immunodeficiency. Clin Immunol Immunopathol. 1979 Sep;14(1):107–120. doi: 10.1016/0090-1229(79)90131-4. [DOI] [PubMed] [Google Scholar]
  15. Joyner A. L., Bernstein A. Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events. Mol Cell Biol. 1983 Dec;3(12):2180–2190. doi: 10.1128/mcb.3.12.2180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Joyner A., Keller G., Phillips R. A., Bernstein A. Retrovirus transfer of a bacterial gene into mouse haematopoietic progenitor cells. Nature. 1983 Oct 6;305(5934):556–558. doi: 10.1038/305556a0. [DOI] [PubMed] [Google Scholar]
  17. Lieberman M., Declève A., Ricciardi-Castagnoli P., Boniver J., Finn O. J., Kaplan H. S. Establishment, characterization and virus expression of cell lines derived from radiation- and virus-induced lymphomas of C57BL/Ka mice. Int J Cancer. 1979 Aug;24(2):168–177. doi: 10.1002/ijc.2910240208. [DOI] [PubMed] [Google Scholar]
  18. Mann R., Mulligan R. C., Baltimore D. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell. 1983 May;33(1):153–159. doi: 10.1016/0092-8674(83)90344-6. [DOI] [PubMed] [Google Scholar]
  19. Manteuil-Brutlag S., Liu S. L., Kaplan H. S. Radiation leukemia virus contains two distinct viral RNAs. Cell. 1980 Mar;19(3):643–652. doi: 10.1016/s0092-8674(80)80041-9. [DOI] [PubMed] [Google Scholar]
  20. Martin D. W., Jr, Gelfand E. W. Biochemistry of diseases of immunodevelopment. Annu Rev Biochem. 1981;50:845–877. doi: 10.1146/annurev.bi.50.070181.004213. [DOI] [PubMed] [Google Scholar]
  21. Miller A. D., Jolly D. J., Friedmann T., Verma I. M. A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4709–4713. doi: 10.1073/pnas.80.15.4709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Orkin S. H., Daddona P. E., Shewach D. S., Markham A. F., Bruns G. A., Goff S. C., Kelley W. N. Molecular cloning of human adenosine deaminase gene sequences. J Biol Chem. 1983 Nov 10;258(21):12753–12756. [PubMed] [Google Scholar]
  23. Paglia D. E., Valentine W. N. Haemolytic anaemia associated with disorders of the purine and pyrimidine salvage pathways. Clin Haematol. 1981 Feb;10(1):81–98. [PubMed] [Google Scholar]
  24. Parker B. A., Stark G. R. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Virol. 1979 Aug;31(2):360–369. doi: 10.1128/jvi.31.2.360-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Polmar S. H., Wetzler E. M., Stern R. C., Hirschhorn R. Restoration of in-vitro lymphocyte responses with exogenous adenosine deaminase in a patient with severe combined immunodeficiency. Lancet. 1975 Oct 18;2(7938):743–746. doi: 10.1016/s0140-6736(75)90726-6. [DOI] [PubMed] [Google Scholar]
  26. Reisner Y., Kapoor N., Kirkpatrick D., Pollack M. S., Cunningham-Rundles S., Dupont B., Hodes M. Z., Good R. A., O'Reilly R. J. Transplantation for severe combined immunodeficiency with HLA-A,B,D,DR incompatible parental marrow cells fractionated by soybean agglutinin and sheep red blood cells. Blood. 1983 Feb;61(2):341–348. [PubMed] [Google Scholar]
  27. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  28. Shimotohno K., Temin H. M. Formation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into DNA of an avian retrovirus. Cell. 1981 Oct;26(1 Pt 1):67–77. doi: 10.1016/0092-8674(81)90034-9. [DOI] [PubMed] [Google Scholar]
  29. Shore A., Dosch H. M., Gelfand E. W. Role of adenosine deaminase in the early stages of precursor T cell maturation. Clin Exp Immunol. 1981 Apr;44(1):152–155. [PMC free article] [PubMed] [Google Scholar]
  30. Southern P. J., Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet. 1982;1(4):327–341. [PubMed] [Google Scholar]
  31. Tabin C. J., Hoffmann J. W., Goff S. P., Weinberg R. A. Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene. Mol Cell Biol. 1982 Apr;2(4):426–436. doi: 10.1128/mcb.2.4.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Thompson L. F., Seegmiller J. E. Adenosine deaminase deficiency and severe combined immunodeficiency disease. Adv Enzymol Relat Areas Mol Biol. 1980;51:167–210. doi: 10.1002/9780470122969.ch4. [DOI] [PubMed] [Google Scholar]
  34. Valerio D., Duyvesteyn M. G., Meera Khan P., Geurts van Kessel A., de Waard A., van der Eb A. J. Isolation of cDNA clones for human adenosine deaminase. Gene. 1983 Nov;25(2-3):231–240. doi: 10.1016/0378-1119(83)90227-5. [DOI] [PubMed] [Google Scholar]
  35. Van der Weyden M. B., Kelley W. N. Human adenosine deaminase. Distribution and properties. J Biol Chem. 1976 Sep 25;251(18):5448–5456. [PubMed] [Google Scholar]
  36. Wei C. M., Gibson M., Spear P. G., Scolnick E. M. Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. J Virol. 1981 Sep;39(3):935–944. doi: 10.1128/jvi.39.3.935-944.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wiginton D. A., Adrian G. S., Friedman R. L., Suttle D. P., Hutton J. J. Cloning of cDNA sequences of human adenosine deaminase. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7481–7485. doi: 10.1073/pnas.80.24.7481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Williams D. A., Lemischka I. R., Nathan D. G., Mulligan R. C. Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse. Nature. 1984 Aug 9;310(5977):476–480. doi: 10.1038/310476a0. [DOI] [PubMed] [Google Scholar]
  39. Yeung C. Y., Frayne E. G., Al-Ubaidi M. R., Hook A. G., Ingolia D. E., Wright D. A., Kellems R. E. Amplification and molecular cloning of murine adenosine deaminase gene sequences. J Biol Chem. 1983 Dec 25;258(24):15179–15185. [PubMed] [Google Scholar]
  40. Yeung C. Y., Riser M. E., Kellems R. E., Siciliano M. J. Increased expression of one of two adenosine deaminase alleles in a human choriocarcinoma cell line following selection with adenine nucleosides. J Biol Chem. 1983 Jul 10;258(13):8330–8337. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES