Plant antimicrobial peptides

Abstract

Plant antimicrobial peptides (AMPs) are a component of barrier defense system of plants. They have been isolated from roots, seeds, flowers, stems, and leaves of a wide variety of species and have activities towards phytopathogens, as well as against bacteria pathogenic to humans. Thus, plant AMPs are considered as promising antibiotic compounds with important biotechnological applications. Plant AMPs are grouped into several families and share general features such as positive charge, the presence of disulfide bonds (which stabilize the structure), and the mechanism of action targeting outer membrane structures.

Introduction

As a part of defense response, plants produce a high number of toxic molecules, including antimicrobial peptides (AMPs), that kill pathogens by interaction with phospholipids and membrane permeabilization. The other group comprises cell-penetrating peptides (CPPs), capable of introducing into cells a variety of cargoes in the absence of specific receptors by interaction at some point with membrane phospholipids. AMPs and CPPs are a part of the nonspecific host defense system and are active against different types of microorganisms (Eudes and Chugh 2008; Rivas et al. 2010; Pelegrini et al. 2011; Hegedus and Marx 2013). Antimicrobial peptides have been described in a wide variety of species including, insects, amphibians, and mammals. They exhibit a wide range of functions ranging from direct antimicrobial properties to immunomodulatory effects (Choi et al. 2012). AMPs have been demonstrated to inactivate prokaryotic cells by targeting a number of essential or metabolic processes at extracellular, plasma membrane, and/or intracellular sites (Yount and Yeaman 2013). Most of the natural antimicrobial peptides are 10 to 50 amino acids (aa) in length, range in size from 2 to 9 kDa, are positively charged, contain a high position of hydrophobic amino acid, and often display a helical structure. AMPs are gene-encoded and they are either constitutively expressed or rapidly transcribed upon induction in eukaryotes by invading microbes and their products, or host cellular compounds, such as cytokines, butyrate, or vitamins (Schauber et al. 2006; Lai and Gallo 2009). These peptides are categorized into distinct families mainly on the basis of their amino acid sequence, identity, number of cysteine residues, and their spacing (Lay and Anderson 2005). On the basis of their electrical charge, plant AMPs can be divided into anionic (AAMPs) and cationic peptides (CAMPs) (Pelegrini et al. 2011).

Plant antimicrobial peptides has been isolated from roots, seeds, flowers, stems, and leaves from a wide variety of species and have demonstrated activities towards phytopathogens, as well as against organisms pathogenic to human, viruses, bacteria, fungi, protozoa, parasites, and neoplastic cells (Montesinos 2007). The repertoire of AMPs synthesized by plants is extremely large with hundreds of different AMPs in some plant species. The main families of AMPs comprise defensins, thionins, lipid transfer proteins, cyclotides, snakins, and hevein-like proteins, according to amino acid sequence homology.

Structural and functional relationships of plant AMPs

Primary and tertiary structure comparison of plant AMPs

In silico analyses revealed some similarities in tertiary structures of plant AMPs, despite significant differences in amino acid sequences between the families (Pelegrini et al. 2011; Fig. 1). Key features of AMPs are high content of cysteine and/or glycine and the presence of disulphide bridges, which are important for enhancing structural stability under stress conditions. Around 17 % of the amino acids in plant AMPs are charged (mainly ariginines and/or lysines, but also aspartic acid and glutamic acid), what seems to play an essential role in activity towards pathogenic bacteria (Hammami et al. 2009; Pelegrini et al. 2011).

Fig. 1.

Three-dimensional structures of selected antimicrobial peptides from different families. The structures were retrieved from RCSB Protein Databank and visualized with UCSF Chimera package (Resource for Biocomputing, Visualization, and Informatics; University of California) (Pettersen et al. 2004)

Mechanism of antibacterial and antifungal action of plant AMPs

Most of the known AMPs act by formation of membrane pores, resulting in ion and metabolite leakage, depolarization, interruption of the respiratory processes, and cell death (Pelegrini et al. 2011). Amphipathic structure and positive charge at physiological pH may be significant features allowing AMPs to interact with membrane lipids. The cationic residues electrostatically attract negatively charged molecules (e.g., anionic phospholipids, lipopolysaccharides, or teichoic acids) allowing the peptide to accumulate on the membrane surface (Pelegrini and Franco 2005). When concentration reaches a threshold value, the collapse begins. Three main models explaining this phenomenon were proposed (Fig. 2): barrel-stave model, the wormhole (or toroid pore) model, and carpet model. In the barrel-stave mechanism, AMPs oligomerize with hydrophobic residues of peptide facing interior of the lipid bilayer and hydrophilic ones oriented towards the lumen of newly formed pore. In the wormhole mechanism, peptide molecules reorient in the membrane during the aggregation dragging of the lipids with them (through electrostatic interactions between head groups of phospholipids and hydrophilic residues of AMPs). Consequently, the membrane is “bend” and joined layers form the toroidal pore. In the carpet mechanism, peptides act like detergents, covering the membrane in an electrostatic manner (in monomeric or oligomeric form). This “carpet” of amphipatic molecules causes a phospholipid displacement, alters membrane properties, and disrupts the membrane (Pelegrini et al. 2011). There are some other models such as the sinking raft model (Pokorny and Almeida 2004), aggregate model (Wu et al. 1999), or the molecular electroporation (Miteva et al. 1999); however, they have not received much attention in the field, are rarely cited, and have not found much experimental confirmation.

Fig. 2.

Frequently cited models for activity of antimicrobial peptides. a AMPs diffusing through solution, b AMPs adsorption to the membrane. After the threshold concentration is achieved, peptide molecules begin to reorient in the lipid bilayer (c). Their further fate may be described using one of three models. The first, depicted in the d is called barrel-stave model. In this scenario, hydrophobic regions of AMPs align with the tails of the lipids and the hydrophilic residues form the inner surface of the forming pore. According to the wormhole model (called also toroidal pore model, shown in e) during peptides aggregation, hydrophilic heads of the lipids are electrostatically dragged by charged residues of AMPs. The membrane bends, two layers merge and form continuous surface surrounding the pore. The carpet model shown in f assumes, that at large concentrations, peptide molecules disrupt the membrane in a detergent-like manner breaking the lipid bilayer into set of separate micelles

There are some differences between antifungal and antibacterial activity, mainly connected with different composition of the target membrane. For example, γ-thionins might bind to glucosylceramides and sphingolipids in fungal membrane (instead of phospholipids being their receptors in bacteria; Pelegrini and Franco 2005). However, many AMPs (e.g., γ-thionin SIα1 from Sorghum bicolor) show activity toward both bacteria and fungi (Hughes et al. 2000; Pelegrini and Franco 2005).

In terms of specificity of plant AMPs–pathogen interactions, still a lot remains unclear. Nevertheless, specific residues could be connected with thionins activity towards different groups of organisms. For example, A2 γ-thionin from Pyrularia pubera (Pp-TH) contains aspartic residue at the position 32 instead of arginine, commonly found in other γ-thionins. The presence of Asp32 was shown to be important for in vitro activity against diverse Gram-negative bacteria (Rhizobium melioti and Xanthomonas campestris) and numerous fungi (Fusarium oxysporum, Plectosphaerella cucumerina, and Botritis cinerea; Villa-Perello et al. 2003; Pelegrini and Franco 2005). Site-directed mutagenesis studies performed to produce new variants of Rs-AFP1 defensin revealed that a variant in which Gly9 or Val39 was replaced with arginine was more active against certain fungi than wild-type Rs-AFP2 (Lay and Anderson 2005).

Detailed description of main families of plant AMPs

Thionins

Thionins are a family of antimicrobial peptides with low molecular weight (about 5 kDa), rich in arginine, lysine, and cysteine residues. Their structure includes two antiparallel α-helices and an antiparallel double-stranded β-sheet with three or four conserved disulfide linkages. They are positively charged at neutral pH. The groove between the α-helices and β-sheets posses the Tyr 13 residue, the membrane interactions of which may be associated with cell leakage which appears to be a common mechanism of cell lysis of thionins (Majewski and Stec 2001). Thionins are toxic against bacteria, fungi, and yeast (Table 1). Around 100 individual thionin sequences have been identified in more than 15 different plant species (in monocots, dicotyledonous, and rosids; Stec 2006). The first thionin was isolated in 1942 by Balls and collaborators from wheat endosperm Triticum aestivum, later called purothionin (Mak and Jones 1976). The name thionins is used for two distinct groups of plant peptides: α-/β-thionins and γ-thionins. The last group (γ-thionins) have much more in common with a large family of membrane active peptides called defensins, found in plants and animals (Stotz et al. 2009). Thionins have a common gene structure with an ~20 aa-long leader peptide and an ~60 aa-long trailing acid peptide, which neutralizes the basic toxin (Stec 2006). Cleavage of the leader peptide is necessary for toxin activation. All thionins are present in almost every crucial plant tissue from endosperm to leaves. Their toxic effect was postulated to arise from lysis of the membranes of attaching cells. The precise mechanism underlying toxicity remains unknown. Antifungal activity of thionins is a result of direct protein–membrane interactions by electrostatic interaction of the positively charged thionin with the negatively charged phospholipids in fungal membranes, and this result in pore formation or a specific interaction with a certain lipid domain (De Lucca et al. 2005). α-/β-thionins are subdivided into five classes; however, all types appear highly homologous at the amino acid level (Stec 2006).

Table 1.

Antimicrobial properties of selected thionins

Protein	Susceptible species	References
Wheat endosperm crude purothionin	Bacteria: Pseudomonas solanacearum Xanthomonas phaseoli Xanthomonas campestris Erwinia amylovora Corynebacterium fascians C. flaccumfaciens C. michiganese C. poinsettiae C. sepedonicum	Fernandez De Caleya et al. (1972)
Wheat endosperm α-purothionin	Fungi: Rhizoctonia solani	Oard et al. (2004)
Viscotoxin A3 and B from leaves and stems of Viscum album L.	Fungi: Fusarium solani Sclerotinia sclerotiorum Phytophtora infestans	Giudici et al. (2004)
Nicotiana attenuate PR-13 thionins	Bacteria: Pseudomonas syringae pv. tomato	Rayapuram et al. (2008)
Pearl millet seed thionin	Fungi: Sclerospora graminicola	Chandrashekhara et al. (2010)
WBeta (thionin) from Triticum aestivum	Fungi: Fusarium solani	Terras et al. ( 1993a, b)
AX1 thionin from Beta vulgaris	Fungi: Cercospora beticola	Kragh et al. (1995)

Types I and II thionins

Type I thionins (purothionins) are present in the endosperm of grains (the family Poaceae), are highly basic, and consist of 45 amino acids, 8 of which are cysteins. Type II thionins (α-hordothionin and β-hordothionin) are slightly less basic than type I, consists of 46–47 amino acids, and were isolated from leaves and nuts of the plant P. pubera (Vernon 1992). Types I and II thionins have four disulfide bonds.

Type III thionins

Type III thionins have 45–46 amino acids and three disulfide bridges and are as basic as type II thionins. They were isolated from the leaves and stems of mistletoe species, such as Viscum album (viscotoxins A1, A2, A3, B, B2, 1-PS, UPS, C1), Phoradendron tomentosum phoratoxins A, B), Phoradendron liga (ligatoxin A), and Dendrophthora clavata (Samuelsson and Pettersson 1970, 1977; Thunberg and Samuelsson 1982).

Type IV thionins

Type IV thionins (crambins) consist of 46 amino acids and three disulfide bonds. Crambin has no charge at neutreal pH and its helices have a significant hydrophobic character. Despite overall hydrophobic character (neutral charge), crambin is amphipathic with two Arg residues. They were isolated from seeds of Crambe abyssinica (Abyssinian cabbage; Schrader-Fisher and Apel 1994).

Type V thionins

Type V thionins are truncated forms of thionins found in some grains like wheat. Hellothionin D isolated from roots of Helleborus purpurascens belongs to this group (Milbradt et al. 2003).

One of the best structurally studied proteins is Viscotoxin isolated from leaves and stems of European mistletoe (V. album). This thionin is toxic against a various number of cells, particularly against tumoral cells. These peptides induce the appearance of imperfections on the surface of membranes that lead to the destabilization and disruption of the membrane bilayer (Stec 2006). From the endosperm of wheat seeds beta-purothionin was isolated, which assume inserts into the hydrophobic core of the lipid layer (Stec 2006). α-(1)-Purothionin is a wheat germ protein and a basic lytic toxin. Thionins are included in the pathogenesis-related (PR) proteins as the PR-13 group (Epple et al. 1995).

Defensins

The first plant defensins were isolated from wheat T. aestivum and barley Hordeum vulgare and initially classified as γ-thionins. Plant defensins are small (ca. 5 kDa), basic, cysteine-rich peptides ranging from 45 to 54 amino acids, and are positively charged. Biological activities reported for plant defensins include antifungal, antibacterial, proteinase, and insect amylase inhibitor activities (Table 2; Wijaya et al. 2000; Stotz et al. 2009). The plant defensins have quite diverse amino acid composition and conserved three-dimensional structure, which comprises a triple-stranded β-sheet with an α-helix in parallel stabilized by four disulfide bridges. Plant defensins are very similar to defense peptides of mammals and insects what suggest their ancient and conserved origin. Generally, plant defensins are composed by one subunit, being found in monomeric forms. On the other hand, the defensins from Pachyrrhizus erosus and other from Vigna unguiculata showed the ability to dimerism (Pelegrini and Franco 2005). The mode of action of plant defensins is still unclear and not all plant defensins have the same mode of action. Probable defensins used glucosylceramides as receptors for fungi cell membrane insertion. Then, repulsion of defensins into cell membrane by their positive charges leads to membrane disruption, membrane destabilization, and ion efflux (Pelegrini and Franco 2005). Plant defensins can be divided in two groups: (1) plant defensins that inhibit fungal growth through morphological distortions of the fungal hyphae and (2) plant defensins that inhibit fungal growth without morphological distortion (Hegedus and Marx 2013). Most plant defensins were isolated from seeds. In radish, defensin RS-AFPs represents 0.5 % of total protein in seeds. Defensins were also isolated from leaves, pods, tubers, fruits, roots, bark, and floral organs of such plants as Heuchera sanguinea (Hs-AFp1), Raphanus sativus (Rs-AFP1), Aesculus hippocastanum (Ah-AMP1), Dahlia merckii (Dm-AMP1), and Clitoria ternatea (Ct-AMP1; De Lucca et al. 2005). Defensins are expressed during normal plant growth and development and induced by environmental factors and biotic and abiotic stress (Pestana-Calsa and Calsa 2011). The defensins gene induced upon pathogen infection has been identified in pea, tobacco, Arabidopsis, and spruce (Lay and Anderson 2005).

Table 2.

Antimicrobial properties of selected plant defensins

Defensin	Susceptible species	Reference
MsDef1 from Medicago sativa	Fungi: Magnaporthe grisea Erwinia carotovora Botrytis cinerea	Spelbrink et al. (2004)
WT 1 from Wasabia japonica L.	Fungi: Magnaporthe oryzae Rizoctonia solani	Lay and Anderson (2005)
Dm-AMP1 from dahlia	Fungi: Fusarium culmorum	Zhu et al. (2007)
Ah-AMP1 from Aesculus hippocastanum	Fungi: Fusarium moniliforme	Terras et al. (1993a, b)
Rs-AFP1 from Raphanus sativus	Fungi: Fusarium culmorum Botritis cinerea	De Lucca et al. (1999)
RsAFP2 from Raphanus sativus	Fungi: Baker's yeast Candida albicans	Thevissen et al. (2012)
Hc-AFP1 Hc-AFP2 HcAFP3 Hc-AFP4 from Heliophila coronopifolia	Fungi: Botrytis cinerea Fusarium solani	De Beer and Viver (2011)
HsAFP1 from Heuchera sanguinea	Fungi: Aspergillus flavus Candida albicans Candida krusei	Thevissen et al. (2007)
Ns-D1 Ns-D2 from Nigella sativa seeds	Fungi: Aspergillus niger Fusarium oxysporum Fusarium graminearum Fusarium culmorum Bipolaris sorokiniana Botritis cinerea	Rogozhin et al. (2011)

Two classes of defensins are produced. The first class, the precursor protein, contains an amino signal peptide that targets the peptide to the extracellular space. The second class of defensins have C-terminal prodomains.

Plant defensins are best known for their antimicrobial activity against a broad spectrum of plant pathogens as bacteria, yeast, oomycetes, and necrotrophic pathogens (Segura et al. 1998; Portieles et al. 2010; van der Weerden et al. 2010). They also show activities important for medical applications as anticancer activity and antiviral activity (Ngai and Ng 2005; Wong and Ng 2005). Plant defensins interact with glucosylceramides in membranes of susceptible yeast and fungi and induce membrane permeabilization and fungal cell death (Thevissen et al. 1996, 2004).

γ-Hordothionin belongs to plant defensins (molecular weight, 5,250 Da; contains four disulfide bridges), which inhibits translation in cell-free systems. The others are defensin PhD1 from Petunia hybrida with antifungal activity and defensins 1 and 2 (VrD1 and VrD2) isolated from the seeds of the mung bean, Vigna radiata (Padovan et al. 2010). However, only VrD1 exhibits insecticidal activity and α-amylase inhibitory activity. PhD1 has 47 residues and five disulfide bonds. Other features of plant defensins are related to the regulation of growth, development, and fertilization (Oomen et al. 2011).

Lipid transfer proteins

In various monocotyledonous and dicotyledonous plant species, the nonspecific small lipid transfer proteins (ns-LTPs) are present that are capable of exchanging lipids between membranes in vitro. ns-LTPs participate in membrane biogenesis; regulation of the intracellular fatty acid pools; involved in defense reactions against phytopathogens, cutin formation, embryogenesis, and symbiosis; and the adaptation of plants to various environmental conditions. Their antifungal mode of action is not yet known. ns-LTP may insert themselves in fungal membranes and form a pore resulting in an efflux of intracellular ions culminating in cell death (Selitrennikoff 2001). All LTPs share a common structural architecture of a hydrophobic cavity enclosed by four α-helices, held in a compact fold by four disulfide bonds (Yeats and Rose 2008).

LTPs bind a large range of lipid molecules to their hydrophobic cavity. These proteins are divided into two subfamilies with relative molecular masses of 9 kDa (LTP1s) and 7 kDa (LTP2s) and they exhibit low overall amino acid sequence similarity (about 30 %). The N-terminal sequence of the 9 kDa ns-LTP show a high homology, both between dicots and monocots, conservation of a specific Val, near-complete conservation of certain Gly, Ser and proresidues, and conservation of hydrophobic residues at specific sites (Yeats and Rose 2008). Almost all ns-LTPs lack tryptophan residues, except for a few isoforms in Arabidopsis and rice that have 1–2 Trp. LTPs were isolated from young aerial organs of Nicotiana tabacum, as well as mung bean and rice. A number of ns-LTPs exhibit antibacterial and antifungal properties in vitro, hence have been classified as the class PR-14 of the pathogenesis-related proteins. Some of ns-LTPs are important allergens in fruits, vegetables, nuts, pollen, and latex (Egger et al. 2010). The ns-LTP from Chinese cabbage, CaNbp10, was found to be a calmodulin-binding protein, regulated by phosphorylation in calcium-dependent manner. CaM-binding domain is localized at the C-terminal region of this protein (Li et al. 2011). Most of these proteins causing 50 % inhibition (EC 50) were in the range of 0.1–1 mmol/L for bacterial pathogen (Clavibacter michiganensis and Pseudomonas solanacearum) and close to 10 mmol/L for the fungal pathogen (Fusarium solani; Table 3).

Table 3.

Antimicrobial properties of selected ns-LTPs

Ns-LTP	Susceptible species	Reference
Ace-AMP1 from Allium cepa	Fungi: Fusarium oxysporum	Cammue et al. (1995)
Cw18 from Hordeum vulgare	Fungi: Fusarium solani	Molina et al. (1993)
LTP-a1 LTP-a2 From the leaves of Columbia wild-type Arabidopsis LTP-s1 LTP-s2 from spinach	Fungi: Fusarium solani Bacteria: Clavibacter michiganensis subsp. sepedonicus Pseudomonas solanacearum	Segura et al. (1993)
Ca-LTP(1)	Fungi: Colletotrichum lindemuthianum Candida tropicalis Other activity: Inhibitor of mammalian α-amylase	Diz et al. (2011)
Cc-LTP-1 from Coffea canephora seeds	Fungi: Candida albicans Candida tropicalis Other activity: Inhibitor of mammalian α-amylase	Zottich et al. (2011)
LTP protein from wheat (Sumai3)	Fungi: Rhizoctonia solani Curvularia lunata Alternaria sp. Bipolaris oryzae Cylindrocladium scoparium Botritis cinerea Sarocladium oryzae	Kirubakaren et al. (2008)
AceAMP1 LTP from onion seeds	Antifungal and antibacterial	Cheng et al. (2011)

Puroindolines

The puroindolines are small basic proteins and contain a unique tryptophan-rich domain. These proteins were isolated from wheat endosperm. They have molecular masses around 13 kDa and contain five disulfide bridges. There are at least two major isoforms called puroindoline (PIN)-a and PIN-b which are encoded by the Pina-D1 and Pinb-D1 genes, respectively. Both proteins contain a backbone of ten conserved Cys residues with a tertiary structure similar to that of LTPs comprised of four α-helices separated by loops of variable lengths, with the tertiary structure held together by five disulphide bridges. Four of them are identical to those in ns-LTPs and the fifth is present in PINs due to the two additional Cys (Gautier et al. 1994). PINs contain cations monovalent and also a unique amphiphilic tryptophan-rich domain that is not found in the ns-LTPs. The Trp residues occupy a surface loop and form probably the membrane lipid-binding site. The puroindolines are the functional components of the wheat grain hardness locus, control kernel texture, and have antifungal activity (Bhave and Morris 2008; Giroux et al. 2003; Dhatwalia et al. 2009; Zhang et al. 2011).

The antimicrobial activity of PINs is related to interactions with cellular membranes (Table 4). Charnet et al. indicated that PIN-1 is able to form ion channels in artificial and biological membranes which display some selectivity toward monovalent cations. The voltage and Ca²⁺ ions modulate channels formation and/or opening (Charnet et al. 2003). Puroindolines may also be membranotoxins that might play a role in the defense mechanism of plants against microbial pathogens.

Table 4.

Antimicrobial properties of selected puroindolines (PINs)

Puroindoline	Susceptible species	Reference
PINA and PINB from wheat	Fungi: Alternaria brassicola Ascophyta pisi Botrytis cinerea Verticillium dahliae Fusarium culmorum Cochliobolus heterostrophus	Marion et al. (2007)
		Dubreil et al. (1998)
		Zhang et al. (2011)
PINA from wheat	Bacteria: Erwinia amylovora	Jing et al. (2003)
From wheat flour Triticum aestivum L.	Bacteria: Staphylococcus aureus Microcococcus luteus Klebsiella sp. Bacillus cereus	Dhatwalia et al. (2009)

Snakins

Peptides called snakins have been isolated from potato tubers. They comprise the cell wall-associated peptide snakin-1 (StSN1) and snakin-2 (StSN2), which are antimicrobial peptides with 63 amino acid residues (Table 5; 6.9 kDa). These peptides show only 38 % sequence similarity and have identical antimicrobial activity against bacterial and fungal pathogens of different plant species. Homologous peptides have been isolated from other plant species. All snakins have 12 conserved cysteine residues and six disulfide bonds (Segura et al. 1999). The mechanism of action of snakins is not known. They do not interact with artificial lipid membranes. The StSN1 gene from potato is constitutively expressed in different tissues during development and does not respond to abiotic or biotic stress. The expression of the StSN2 is locally induced by wounding and shows differential responses to pathogen infection. The snakin peptides are basic and rich in Cys residues, which may form six disulphide bridges that stabilize their structure (Berrocal-Lobo et al. 2002).

Table 5.

Antimicrobial properties of selected snakins

Snakins	Susceptible species	References
Snakins (StSN1 and StSN2) from potato S. tuberosum cv Jaerla	Fungi: Botrytis cinerea Fusarium solani Fusarium culmorum Fusarium oxysporum f.sp conglutinans Fusarium oxysporum f.sp lycopersici Plectosphaerella cucumerina Colletotrichum graminicola Colletotrichum lagenarium Bipolaris maydis Aspergillus flavus Bacteria: Clavibacter michiganensis Ralstonia solanacearum Ervinia chrysanthemi a Rhizobium meliloti b	Berrocal-Lobo et al. (2002)

^aNot active at concentration: <20 mmol/L

^bNot tested for StSN1

StSN1 amino acid sequence alignments show similarity with members of the tomato GAST family (gibberellic acid stimulated transcript) and Arabidopsis GASA family (gibberellic acid stimulated in Arabidopsis) and it was classified as a member of snaking/GASA family (Almasia et al. 2010). Homologous genes have been identified in a wide range of species within monocotyledonous and dicotyledonous plants (Almasia et al. 2010). Snakin/GASA genes encode small proteins in which three distinct domains can be defined: a putative signal peptide of 18–29 residues, a variable region displaying high divergence between family members, both in amino acid composition and sequence length, and a C-terminal region of ~60 aa containing 12 cysteine residues in conserved positions named GASA domain (Nahirñak et al. 2012).

Snakin/GASA proteins are expressed in different plant organs. Their functions are not completely elucidated and little is known about their mode of action. Most of Snakin/GASA genes are regulated by plant hormones and participate in hormonal signaling pathways modulating hormonal levels and responses (Nahirñak et al. 2012). Members of this family are also implied in diverse processes including defense, cell division, cell elongation, and transition to flowering.

Cyclotides

The cyclotides are group of naturally occurring circular proteins that have been discovered in bacteria, plants, and animals (Pelegrini et al. 2007; Craik 2010). Cyclotides appear to have high sequence similarities and a structural identity. Plant cyclotides comprise 28–37 amino acids, contain a head-to-tail cyclised backbone, and three intramolecular disulfide bonds arranged in a cysteine backbone knot topology (cyclic cysteine knot, CCK). The cysteine knot is formed by the disulfide bonds Cys-1-Cys-4 and Cys-2-Cys-5 and their interconnecting backbone form a ring that is penetrated by Cys-3-Cys-6 disulfide bonds (Colgrave and Craik 2004). CCK is largely responsible for the exceptional stability of cyclotides. It forces the hydrophobic parts of the protein to be exposed at the molecular surface. The hydrophobic residues form a patch on the surface, making the overall structure amphipathic (Pränting et al. 2010). They are resilient to various proteolytic and degradative processes (Ireland et al. 2010). The cyclotide structures contain six backbone loops between the conserved Cys residues and different degrees of sequence diversity in the different loops (Ireland et al. 2010). For example, loops 1 and 4 are highly conserved in both size and residue type, whereas the other loops are more variable. Cyclotides were isolated from the plants belonging to family Violaceae, Rubiaceae, Cucurbitaceae, and Poaceae belong to Asterids, Rosids, and Monocots (Gruber 2010). Based on structural similarities, cyclotides are divided into two subfamilies: Mobius and the bracelets based on the presence or absence of a cis-proline, respectively (Craik et al. 1999). Another type of cyclotide structure has katata B8 isolated from Oldenlandia affinis. It appears to be a hybrid between Mobius and bracelet subfamilies (Pelegrini et al. 2007). The plant cyclotides are gene-encoded peptides generated via ribosomal biosynthetic pathways. The cyclotide precursor contains an endoplasmic reticulum ER signal, a pro-region, an N-terminal repeat (NTR), and a cyclotide sequence domain, followed by a short tail (Craik 2010). Individual cyclotide genes encode between one and three repeats of the NTR and cyclotide domain to form multiple cyclotide from a single precursor.

The NTR region has the amphipathic helical nature and might assist in directing the connect folding of the cyclotide domain (Ireland et al. 2010). The role of C-terminal region (CTR) is unclear. The conserved Asn (or Asp) residue in this region suggests that this part of protein is a target of an asparaginyl endoproteinase. The first described cyclotide kalata B1 was isolated from the plant O. affinis (Mylne et al. 2010). Kalata B1 was used by women in Africa to accelerate labor and childbirth. These peptides have a diverse range of biological activities, including uterotonic, anti-HIV, antimicrobial, insecticidal, antihelmintic, and molluscidal properties (Table 6; Craik 2010). Their natural function appears to be as plant defense molecules based on their insecticidal properties (Gruber 2010). Thus, cyclotides have potential applications in both the pharmaceutical and agricultural industries. The cyclotides Vitri isolated from Viola tricolor demonstrated cytotoxicity to human lymphoma and myeloma cells. Similarly, cycloviolacin H4 isolated from Viola hederaceae is able to cause hemolysis in human erythrocytes (Pelegrini et al. 2007). It has been suggested that membrane interactions might be involved in the various biological activities of cyclotides; however, the mechanism of their action remains unknown. These proteins have specific membrane-disrupting activity (Svangård et al. 2007; Burman et al. 2011). Kalata B1 interacts directly with the membrane by targeting phosphatidylethanolamine phospholipids, probably leading to membrane bending and vesicle formation. This protein together with cyclotide Momordica cochinensis trypsin inhibitor II (McoTI-II) extracted from seeds and sunflower trypsin inhibitor I (SFT1) from seeds belong also to cyclic cell-penetrating peptides CCPs (Greewood et al. 2007). McoTI-II has been reported to be internalized into cells by macropinocytosis, probably by interacting with phosphatidylinositides and phosphatidic acid, but the specific mechanism by which this occurs is not known (Cascales et al. 2011). The mechanism of penetration of SFTI-1 across the plasma membrane of living cell remains unresolved but is independent of phospholipid and differs from McoTI-II and kalata B1 (Greewood et al. 2007).

Table 6.

Biological activity of selected cyclotides

Cyclotide	Activity	References
Kalata B1 from Oldenlandia affinis	Insecticidal, molluscidal, hemolytic, nematocidal, antibacterial, anti-HIV	Jennings et al. (2001)
		Plan et al. (2008)
		Daly et al. (2004)
		Craik (2012)
Kalata B2 from Oldenlandia affinis	Insecticidal, molluscicidal, nematocidal, antibacterial,	Plan et al. (2008) Ovesen et al. (2011)
		Craik (2012)
Cyrulin A&B from Chassalia parviflora	Hemolytic, antibacterial, anti-HIV	Gustafson et al. (1994)
cycloviolacin O1 from Viola odorata	Nematocidal, molluscidal	Craik et al. (2006)
Cycloviolacin O2 from Viola odorata	Gram-negative bacteria	Pränting et al. (2010)
MCoTI-II from Momordica cochinensis	Trypsin inhibitor	Thongyoo et al. (2009)

Hevein-like proteins

Hevein is a small 4.7 kDa, cysteine-rich, chitin-binding peptide present in the lutoid bodies of rubber tree Hevea brasiliensis latex (Van Parijs et al. 1991). This protein inhibits the hyphal growth of fungi by binding to chitin. Other hevein-like proteins with antimicrobial activity have been identified in different plants (Koo et al. 1998; Kiba et al. 2003; Huang et al. 2004; Porto et al. 2012; Table 7). Hevein-like peptides are small (43 amino acid residues) chitin-binding peptides. All known chitin-binding proteins contain a common structural motif of 20–40 amino acids with several cysteine and glycine residues at conserved positions named the chitin-binding domain, which is responsible for binding the carbohydrate. The hevein-like AMPs differ in the number of disulfide bonds. Most of them possess eight cysteine residues forming four disulfide bonds; for example, hevein homolog isolated from the seeds of Pharabitis nil L. and Avena sativa (Li and Claeson 2003). The other contains only six cysteine residues, as hevein-like proteins from Amaranthus caudatus seeds or Ginkgo biloba (Huang et al. 2000). Only a few hevein-like plant AMPs with ten cysteins have been described. They were isolated from the bark of Eucommia ulmoides Oliv, Euonymus europaeus L. and from seeds of Triticum kiharae (Van den Berg et al. 2002; Huang et al. 2002; Odintsova et al. 2009).

Table 7.

Antimicrobial properties of selected hevein-like AMPs

Hevein-like AMP	Susceptible species	References
IWF4 from Beta vulgaris	Fungi: Cercospora beticola	Nielsen et al. (1997)
Ac-AMP1 from Amaranthus caudatus	Fungi: Fusarium culmorum	Broekaert et al. (1992)
EAFP1 EAFP2 from bark Eucommia ulmoides bark	Fungi: Phytophthora infestans Ascopchyta lycopersici Verticillium dahliae Giberella zeae Alternaria nicotianae Fusarium moniliforme Fusarium oxysporum Colletotrichum gossypii Bacteria: Pseudomonas syringae	Huang et al. (2002)
PMAPI from paper mulberry (Broussonetia papyrifera syn. Morus papyrifera L.)	Fungi: Trichoderma viride	Zhao et al. (2011)
WjAMP1 from leaves of Wasabia japonica L.	Fungi: Botrytis cinerea Fusarium solani Magnaporthe grisea Alternaria alternata Bacteria: Escherichia coli Agrobacterium tumefaciens Pseudomonascichorii P. plantarii (Burkholderia plantarii) P. glumae (B. glumae)	Kiba et al. (2003)

Two AMPs from seeds of Pharbitis nil (Pn-AMP1 and Pn-AMP2) exhibited potent antifungal activities against both chitin-containing and nonchitin-containing fungi in cell wall. The Pn-AMPs penetrated rapidly into fungal hyphae and caused burst of hyphal tips, disruption of the fungal membrane, and linkage of cytoplasmic materials (Koo et al. 1998).

An antifungal peptide from leaves of G. biloba, designated GAFP, could also cause increased hyphal membrane permeabilization and exhibited antifungal activity towards Fusarium graminearum, Fusarium moniliforme, Pellicularia sasakii Ito, and Alternaria alternata (Huang et al. 2000). The high inhibitory activity of antifungal hevein-type peptides from T. kiharae seeds (WAMP-1a and WAMP-1b) was also observed against Fusarium salani, Fusarium oxosporum, Fusarium verticillioides, Neurospora crassa, B. cinerea and Bipolaris sorokiniana, and bacteria C. michiganensis, Erwinia carotovora, and Pseudomonas syringae (Huang et al. 2002).

Other plant AMPs

Ib-AMPs

Ib-AMPs are the four smallest (20-mer) antifungal and antibacterial peptides isolated from the seeds of Impatiens balsamina. They contain a well-defined loop structure stabilized by two disulfide bonds (Patel et al. 1998).

Knottin-type peptides

The knottin type antifungal peptides have been isolated from plants Mirabilis jalapa L. (Mj-AMP1) and from Phytolacca americana (PAFP-S; Cammue et al. 1992; Gao et al. 2001). The structure of PAFP-S consists of a triple-stranded, antiparallel beta-sheet with a long loop region connecting β-strands 1 and 2. This peptide from garden pea (PA1b) acts on insecticides through inhibition of vacuolar ATPase (Chouabe et al. 2011).

2S albumin proteins

The 2S albumin is a water-soluble storage protein group with low molecular weight, rich in glutamine. These proteins have the characteristic molecular weight, cationic residues, and disulfide bonds of antimicrobial peptides. The 2S alubmins are encoded by a multigene family, leading to several isoforms that are postranslationally modified, mainly related to proteolytic processes (Candido et al. 2011). The 2S albumins are synthesized as a single large precursor polypeptide of 18–21 kDa. The processing of the molecule gives rise to two subunits of 8–14 and of 3–10 kDa. Structurally, they have four alfa helices and four disulfide bonds as found in the alfa-amylase/trypsin inhibitors and nonspecific lipid transfer proteins. They are widely present in monocotyledonous and dicotyledonous seeds (Candido et al. 2011). Some of these molecules can play a physiological role in plant defense. The heterodimeric antifungal 2S albumins have been isolated from the seeds of Malva parviflora, Passiflora edulis f. flavicarpa, and R. sativus (Terras et al. 1992; Wang and Bunkers 2000; Agizzio et al. 2003). Furthermore, peptide LJAMP1 from seed extract of matherwort (Leonurus japonicus) with the similar sequence to members of the 2S albumin class was identified. The LJAMP1 have activity against the fungi A. alternata, Cercospora personata, and Aspergillus niger (Yang et al. 2007). The other protein (MiAMP2) was extracted from seeds of Macadamia integrifolia. MiAMP2 showed antimicrobial activity against a wide variety species of phytopathogenic fungi such as F. oxysporum, Alternaria helianthi, Cetratocystis paradoxa, Cercospora nicotianae, Chalara elegans, Leptosphaeria maculans, Sclerotinia sclerotiorum, Verticillium dahliae, Phytophthora cryptogea, and Phytophthora parasitica nicotianae against the yeast Saccharomyces cerevisiae and phytopathogenic bacteria C. michiganensis, Ralstonia solanacearum, and Escherichia coli (Candido et al. 2011). Nevertheless, the mechanism by which 2S proteins inhibit fungal and bacterial growth is not very well understood.

Cell-penetrating peptides

CPPs, alternatively known as protein transduction domains (PTDs), facilitate the transport of cargoproteins through the cell membrane into live cell (Koren and Torchilin 2012; Milletti 2012). The CPPs are able to penetrate the cell membrane at low micromolar concentrations in vitro and in vivo without using any receptors and without causing any significant membrane damage (Nasrollahi et al. 2012). They can be conjugated with a cargo (nucleic acids, oligonucleotide, peptide sequence, and polisaccharides), efficiently deliver it inside cells and thus are potentially useful agents in drug delivery applications (Greewood et al. 2007; Cascales et al. 2011; Eggenberger et al. 2011). The various CPPs and CPP-cargo conjugates can enter cells using different endocytotic mechanisms (macropinocytosis, clathrin-mediated endocytosis, caveolae/lipid raft-mediated endocytosis, and clathrin/caveolae-independent endocytosis) and can end up in different subcellular compartments (Koren and Torchilin 2012; Milletti 2012). These short, positively charged peptides have different amino acid sequences, but all contain a transduction domain and have 30–100 % cationic Arg and Lys residues (Hong and Su 2011). Even though CPPs have a great sequence variety, it is possible to identify three major classes: cationic, amphipathic, and hydrophobic (Milletti 2012). Such peptides and proteins are derived as partial sequences from transcription factors, bacterial or viral surface proteins, toxins, amphipathic helix-forming peptides, and from ligands of membrane-bound receptors or adhesion proteins. One of the plant CPPs is the sweet arrow peptide which derived from the proline-rich N-terminal repetitive domain of gamma-zei, a storage protein of maize, which has been shown to interact with membrane (Veldhoen et al. 2008). Polyprolines adopt a well-defined helical structure (polyproline II) in water; but unlike α-helix, it is left-handed with 3.0 residues per turn (Fernández-Carneado et al. 2004). For its cellular entry, a clathrin-independent pathway through lipid raft-mediated endocytosis was proposed (Veldhoen et al. 2008). Members of this family are widely present in plants and animals but are absent in yeast. CPPs according to their origin can be grouped into three classes. The first class comprises CPPs originated from naturally occurring proteins, the second consists of “chimeric CPPs” composed of different domains, and the third class contains “model CPPs”, which were developed according to structure and function relationships without any homology to natural sequences (Veldhoen et al. 2008).

Plant AMPs potential for pharmacy and biotechnology

AMPs are encoded by small genes with conserved sequences; therefore, gene amplification and transgenesis are one of the feasible ways to increase production and enhance specific activity of selected peptides. Therefore, AMPs are also widely applied in the development of transgenic crops.

In many studies, it has been demonstrated that transgenic expression of plant defensins leads to protection of vegetative tissues against pathogen attack (Thomma et al. 2002). For example, the Rs-AFP2 radish defensin was expressed in tobacco and tomato and confers protection against Alternaria longipes (Terras et al. 1995) and Mj-AMP1 jalapa defensin expressed in tomato protects against Alternaria solani (Schaefer et al. 2005). The hevein Pn-AMP expressed in tobacco protects against P. parasitica (Koo et al. 2002), and constitutive expression of an alfalfa defensin in potato provided a robust resistance against the agronomically important fungus V. dahliae under field conditions (Gao et al. 2000). There are many other examples of such transgenic expression of different plant AMPs (Montesinos 2007). Possibly, the antimicrobial activity of defensins in vivo can even be enhanced due to the synergistic interaction with other defense components (Thomma et al. 2002).

Also thionins are important tools for genetic improvement and development of transgenic plants expressing higher levels of thionins, increasing the pathogenic resistance and reducing crop losses in agriculture, what could lead to decreasing the necessity of enhanced quantities of pesticide used on agriculture (Pelegrini and Franco 2005).

Another example are cyclotides, which have potential applications in both the pharmaceutical and agricultural industries. Cyclotides could be also invaluable in the development of novel antibiotics and bioinsecticides, like kalata B1, where polar and/or charged residues were modified (Clark et al. 2006; Pelegrini et al. 2007).

Cell-penetrating peptides are also highly promising candidates for intracellular drug delivery, RNA, DNA, and nanoparticles in a nondestructive manner. CPPs have been shown to facilitate delivering a wide variety of biomolecules across the skin. The enormous potential of this technology resides in the high efficiency and relatively low toxicity of CPPs conjugated to bioactive cargoes. Different CPPs can be successfully used for the delivery of high molecular weight drugs into cells as well as for vaccine development. The application of CPPs in pharmaceutical formulations is becoming increasingly popular with a great potential in transdermal drug delivery systems (Nasrollahi et al. 2012).

Concluding remarks

Plant AMPs are diverse peptides differing in their amino acid composition and structure that generally display rapid killing and broad spectrum antimicrobial activities. Therefore, AMPs have a high potential for therapeutic use in healthcare and agriculture, and can be used as natural antibiotics as alternative for their chemical counterparts, for protection of plants, and/or animals against diseases. AMPs offer a good alternative for treating infections in relation to conventional antibiotics based on their broad spectrum activity and efficiency (Pinheiro da Silva and Machado 2012). Despite their many promising features, not one AMP has yet reached the status of a clinically approved drug. However, the cationic AMPs have been applied in the formation of aerosol sprays for patients with cystic fibrosis.

Numerous transgenic plants expressing AMPs that confer different degrees of protection against diseases have been developed; therefore, AMPs could play strong roles in agriculture as plant protection products. Unfortunately, the commercial cultivars have not been marketed because of regulatory limitations and social concerns. The other problems comprise the intrinsic toxicity and low stability of some of the compounds and the need for inexpensive products in plant protection. Therefore, future areas of commercial plant AMPs use consist of developing less toxic and more stable compounds as well as decreasing production costs mainly by improving biotechnological procedures or preparative peptide synthesis (Montesinos 2007).

Abbreviations

aa

Amino acid(s)

AMP

Antimicrobial peptide(s)

approx.

Approximately

CCK

Cyclic cysteine knot

CPP

Cell-penetrating peptide(s)

CTR

C-terminal repeat

ER

Endoplasmic reticulum

GASA

Gibberellic acid stimulated in Arabidopsis

GAST

Gibberellic acid stimulated transcript

kDa

Kilodalton(s)

McoTI-II

Momordica cochinensis trypsin inhibitor II

ns-LTP

Nonspecific lipid transfer protein

NTR

N-terminal repeat

PIN

Puroindoline(s)

PTD

Protein transduction domain(s)

SFT1

Sunflower trypsin inhibitor I

StSN1

Snakin1

StSN2

Snakin2

GAFP

Ginkgo biloba antifungal peptide

PAFP-S

Phytolacca anifungal peptide