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. 1985 Feb;82(3):879–882. doi: 10.1073/pnas.82.3.879

Activation of an N-ras gene in acute myeloblastic leukemia through somatic mutation in the first exon.

C Gambke, A Hall, C Moroni
PMCID: PMC397150  PMID: 3856237

Abstract

A transforming N-ras gene has been cloned from acute myeloblastic leukemia bone marrow cells, in parallel with the N-ras gene derived from fibroblasts of the same patient. N-ras derived from fibroblasts lacked focus-forming activity in NIH/3T3 cells, indicating that gene activation in the leukemia cells must have occurred by a somatic event. Construction of chimeric molecules between the transforming and the normal N-ras genes and subsequent biological and sequence analysis of these constructs revealed that the transforming gene was altered by a point mutation changing amino acid 12 of the N-ras protein from glycine to aspartic acid.

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Selected References

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