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. 1985 Feb;82(4):1035–1039. doi: 10.1073/pnas.82.4.1035

Two alkaline phosphatase genes positioned in tandem in Bacillus licheniformis MC14 require different RNA polymerase holoenzymes for transcription.

F M Hulett, P Z Wang, M Sussman, J W Lee
PMCID: PMC397188  PMID: 3856244

Abstract

Southern transfer analysis of Bacillus licheniformis MC14 DNA, using as probe a DNA fragment from within the coding region of a previously cloned alkaline phosphatase (APase) gene, revealed a second area of hybridization adjacent to the cloned APase gene. A second APase gene (APase II) was subcloned from the same plasmid clone, pMH8, from which the first APase gene (APase I) had been subcloned. The two genes are arranged in tandem with several hundred base pairs separating them. Immunoblot analysis showed that both code for Mr 60,000 proteins that crossreact with anti-APase. Both proteins enzymatically cleave 5-bromo-4-chloro-3-indolyl phosphate. In vitro transcription showed that APase I and APase II are transcribed in the same direction but that the two genes require different forms of Bacillus RNA polymerase: sigma 55- and sigma 37-containing RNA polymerase holoenzymes, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. An G., Friesen J. D. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene. 1980 Dec;12(1-2):33–39. doi: 10.1016/0378-1119(80)90013-x. [DOI] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Campen R. K., Duester G. L., Holmes W. M., Young J. M. Organization of transfer ribonucleic acid genes in the Escherichia coli chromosome. J Bacteriol. 1980 Dec;144(3):1083–1093. doi: 10.1128/jb.144.3.1083-1093.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cashel M., Freese E. Excretion of alkaline phosphatase of Bacillus subtilis. Biochem Biophys Res Commun. 1964 Aug 11;16(6):541–544. doi: 10.1016/0006-291x(64)90189-5. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Downard J. S., Kupfer D., Zusman D. R. Gene expression during development of Myxococcus xanthus. Analysis of the genes for protein S. J Mol Biol. 1984 Jun 5;175(4):469–492. doi: 10.1016/0022-2836(84)90180-3. [DOI] [PubMed] [Google Scholar]
  7. Ghosh B. K., Wouters J. T., Lampen J. O. Distribution of the sites of alkaline phosphatase(s) activity in vegetative cells of Bacillus subtilis. J Bacteriol. 1971 Nov;108(2):928–937. doi: 10.1128/jb.108.2.928-937.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Glynn J. A., Schaffel S. D., McNicholas J. M., Hulett F. M. Biochemical localization of the alkaline phosphatase of Bacillus licheniformis as a function of culture age. J Bacteriol. 1977 Feb;129(2):1010–1019. doi: 10.1128/jb.129.2.1010-1019.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldfarb D. S., Wong S. L., Kudo T., Doi R. H. A temporally regulated promoter from Bacillus subtilis is transcribed only by an RNA polymerase with a 37,000 dalton sigma factor. Mol Gen Genet. 1983;191(2):319–325. doi: 10.1007/BF00334833. [DOI] [PubMed] [Google Scholar]
  10. Guan C. D., Wanner B., Inouye H. Analysis of regulation of phoB expression using a phoB-cat fusion. J Bacteriol. 1983 Nov;156(2):710–717. doi: 10.1128/jb.156.2.710-717.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Haldenwang W. G., Losick R. Novel RNA polymerase sigma factor from Bacillus subtilis. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7000–7004. doi: 10.1073/pnas.77.12.7000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hansa J. G., Laporta M., Kuna M. A., Reimschuessel R., Hulett F. M. A soluble alkaline phosphatase from Bacillus licheniformis MC14. Histochemical localization, purification, characterization and comparison with the membrane-associated alkaline phosphatase. Biochim Biophys Acta. 1981 Feb 13;657(2):390–401. doi: 10.1016/0005-2744(81)90325-9. [DOI] [PubMed] [Google Scholar]
  13. Higgins C. F., Ames G. F. Two periplasmic transport proteins which interact with a common membrane receptor show extensive homology: complete nucleotide sequences. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6038–6042. doi: 10.1073/pnas.78.10.6038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hulett F. M. Cloning and characterization of the Bacillus licheniformis gene coding for alkaline phosphatase. J Bacteriol. 1984 Jun;158(3):978–982. doi: 10.1128/jb.158.3.978-982.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hulett F. M., Schaffel S. D., Campbell L. L. Subunits of the alkaline phosphatase of Bacillus licheniformis: chemical, physicochemical, and dissociation studies. J Bacteriol. 1976 Nov;128(2):651–657. doi: 10.1128/jb.128.2.651-657.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Inouye H., Michaelis S., Wright A., Beckwith J. Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro. J Bacteriol. 1981 May;146(2):668–675. doi: 10.1128/jb.146.2.668-675.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Inouye S., Ike Y., Inouye M. Tandem repeat of the genes for protein S, a development-specific protein of Myxococcus xanthus. J Biol Chem. 1983 Jan 10;258(1):38–40. [PubMed] [Google Scholar]
  18. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 1979;68:268–280. doi: 10.1016/0076-6879(79)68019-9. [DOI] [PubMed] [Google Scholar]
  19. Landick R., Anderson J. J., Mayo M. M., Gunsalus R. P., Mavromara P., Daniels C. J., Oxender D. L. Regulation of high-affinity leucine transport in Escherichia coli. J Supramol Struct. 1980;14(4):527–537. doi: 10.1002/jss.400140410. [DOI] [PubMed] [Google Scholar]
  20. Lodish H. F., Rothman J. E. The assembly of cell membranes. Sci Am. 1979 Jan;240(1):48–63. doi: 10.1038/scientificamerican0179-48. [DOI] [PubMed] [Google Scholar]
  21. Losick R., Pero J. Cascades of Sigma factors. Cell. 1981 Sep;25(3):582–584. doi: 10.1016/0092-8674(81)90164-1. [DOI] [PubMed] [Google Scholar]
  22. McNicholas J. M., Hulett F. M. Electron microscope histochemical localization of alkaline phosphatase(s) in Bacillus licheniformis. J Bacteriol. 1977 Jan;129(1):501–515. doi: 10.1128/jb.129.1.501-515.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moran C. P., Jr, Lang N., Banner C. D., Haldenwang W. G., Losick R. Promoter for a developmentally regulated gene in Bacillus subtilis. Cell. 1981 Sep;25(3):783–791. doi: 10.1016/0092-8674(81)90186-0. [DOI] [PubMed] [Google Scholar]
  24. Moran C. P., Jr, Lang N., Losick R. Nucleotide sequence of a Bacillus subtilis promoter recognized by Bacillus subtilis RNA polymerase containing sigma 37. Nucleic Acids Res. 1981 Nov 25;9(22):5979–5990. doi: 10.1093/nar/9.22.5979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Roberts T. M., Lauer G. D. Maximizing gene expression on a plasmid using recombination in vitro. Methods Enzymol. 1979;68:473–482. doi: 10.1016/0076-6879(79)68036-9. [DOI] [PubMed] [Google Scholar]
  26. Schaffel S. D., Hulett F. M. Alkaline phosphatase from Bacillus licheniformis. Solubility dependent on magnesium, purification and characterization. Biochim Biophys Acta. 1978 Oct 12;526(2):457–467. doi: 10.1016/0005-2744(78)90137-7. [DOI] [PubMed] [Google Scholar]
  27. Silhavy T. J., Benson S. A., Emr S. D. Mechanisms of protein localization. Microbiol Rev. 1983 Sep;47(3):313–344. doi: 10.1128/mr.47.3.313-344.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  29. Spencer D. B., Chen C. P., Hulett F. M. Effect of cobalt on synthesis and activation of Bacillus licheniformis alkaline phosphatase. J Bacteriol. 1981 Feb;145(2):926–933. doi: 10.1128/jb.145.2.926-933.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Spencer D. B., Hansa J. G., Stuckmann K. V., Hulett F. M. Membrane-associated alkaline phosphatase from Bacillus licheniformis that requires detergent for solubilization: lactoperoxidase 125I localization and molecular weight determination. J Bacteriol. 1982 May;150(2):826–834. doi: 10.1128/jb.150.2.826-834.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Spencer D. B., Hulett F. M. Lactoperoxidase-125I localization of salt-extractable alkaline phosphatase on the cytoplasmic membrane of Bacillus licheniformis. J Bacteriol. 1981 Feb;145(2):934–945. doi: 10.1128/jb.145.2.934-945.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stahl M. L., Ferrari E. Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation. J Bacteriol. 1984 May;158(2):411–418. doi: 10.1128/jb.158.2.411-418.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Strongin A. Y., Izotova L. S., Abramov Z. T., Gorodetsky D. I., Ermakova L. M., Baratova L. A., Belyanova L. P., Stepanov V. M. Intracellular serine protease of Bacillus subtilis: sequence homology with extracellular subtilisins. J Bacteriol. 1978 Mar;133(3):1401–1411. doi: 10.1128/jb.133.3.1401-1411.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wawrousek E. F., Hansen J. N. Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis. J Biol Chem. 1983 Jan 10;258(1):291–298. [PubMed] [Google Scholar]
  35. Wong S. L., Doi R. H. Peptide mapping of Bacillus subtilis RNA polymerase alpha factors and core-associated polypeptides. J Biol Chem. 1982 Oct 25;257(20):11932–11936. [PubMed] [Google Scholar]
  36. Wong S. L., Price C. W., Goldfarb D. S., Doi R. H. The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1184–1188. doi: 10.1073/pnas.81.4.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Yokota T., Sugisaki H., Takanami M., Kaziro Y. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Gene. 1980 Dec;12(1-2):25–31. doi: 10.1016/0378-1119(80)90012-8. [DOI] [PubMed] [Google Scholar]

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