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. 1985 Feb;82(4):1214–1218. doi: 10.1073/pnas.82.4.1214

Depletion of mucosal mast cell protease by corticosteroids: effect on intestinal anaphylaxis in the rat.

S J King, H R Miller, G F Newlands, R G Woodbury
PMCID: PMC397225  PMID: 3883354

Abstract

Rats primed by infection with the intestinal nematode Nippostrongylus brasiliensis and challenged intravenously with soluble whole-worm antigen undergo systemic anaphylactic shock. The primary lesions are in the gut and include increased permeability of the mucosa together with release, into enteric secretions, of a mucosal mast cell (MMC)-specific serine proteinase, rat mast cell protease II (RMCP-II). This enzyme is also released into the blood of shocked rats. These manifestations of anaphylaxis were abolished in rats previously treated with corticosteroids (methylprednisolone acetate, 25 mg per kg of body weight, 48 and 24 hr before i.v. challenge with antigen). Suppression of the response was associated with depletion of RMCP-II and of MMC from the intestinal mucosa. Depletion occurred 4-24 hr after treatment with as little as 1 mg of methylprednisolone per kg. By contrast, neither connective tissue mast cells nor serum levels of parasite-specific IgE were depleted in rats given 2 X 25 mg of methylprednisolone per kg. The capacity of unprimed treated rats to mount passive cutaneous anaphylaxis was, however, impaired.

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Selected References

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  1. BENDITT E. P., SCHILLER S., WONG H., DORFMAN A. Influence of ACTH and cortisone upon alteration in capillary permeability induced by hyaluronidase in rats. Proc Soc Exp Biol Med. 1950 Dec;75(3):782–784. doi: 10.3181/00379727-75-18341. [DOI] [PubMed] [Google Scholar]
  2. Barry B. W., Woodford R. Comparative bio-availability of proprietary topical corticosteroid preparations; vasoconstrictor assays on thirty creams and gels. Br J Dermatol. 1974 Sep;91(3):323–338. doi: 10.1111/j.1365-2133.1974.tb12903.x. [DOI] [PubMed] [Google Scholar]
  3. Blackwell G. J., Carnuccio R., Di Rosa M., Flower R. J., Parente L., Persico P. Macrocortin: a polypeptide causing the anti-phospholipase effect of glucocorticoids. Nature. 1980 Sep 11;287(5778):147–149. doi: 10.1038/287147a0. [DOI] [PubMed] [Google Scholar]
  4. Blackwell G. J., Flower R. J. Inhibition of phospholipase. Br Med Bull. 1983 Jul;39(3):260–264. doi: 10.1093/oxfordjournals.bmb.a071830. [DOI] [PubMed] [Google Scholar]
  5. Church M. K., Miller P. Time courses of the anti-anaphylactic and anti-inflammatory effects of dexamethasone in the rat and mouse. Br J Pharmacol. 1978 Apr;62(4):481–486. doi: 10.1111/j.1476-5381.1978.tb07751.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Danon A., Assouline G. Inhibition of prostaglandin biosynthesis by corticosteroids requires RNA and protein synthesis. Nature. 1978 Jun 15;273(5663):552–554. doi: 10.1038/273552a0. [DOI] [PubMed] [Google Scholar]
  7. Daëron M., Sterk A. R., Hirata F., Ishizaka T. Biochemical analysis of glucocorticoid-induced inhibition of IgE-mediated histamine release from mouse mast cells. J Immunol. 1982 Sep;129(3):1212–1218. [PubMed] [Google Scholar]
  8. Flower R. J., Blackwell G. J. Anti-inflammatory steroids induce biosynthesis of a phospholipase A2 inhibitor which prevents prostaglandin generation. Nature. 1979 Mar 29;278(5703):456–459. doi: 10.1038/278456a0. [DOI] [PubMed] [Google Scholar]
  9. Grosman N., Jensen S. M. Influence of glucocorticoids on histamine release and 45calcium uptake by isolated rat mast cells. Agents Actions. 1984 Jan;14(1):21–30. doi: 10.1007/BF01966828. [DOI] [PubMed] [Google Scholar]
  10. Heiman A. S., Crews F. T. Hydrocortisone selectively inhibits IgE-dependent arachidonic acid release from rat peritoneal mast cells. Prostaglandins. 1984 Feb;27(2):335–343. doi: 10.1016/0090-6980(84)90084-4. [DOI] [PubMed] [Google Scholar]
  11. Hirata F., Schiffmann E., Venkatasubramanian K., Salomon D., Axelrod J. A phospholipase A2 inhibitory protein in rabbit neutrophils induced by glucocorticoids. Proc Natl Acad Sci U S A. 1980 May;77(5):2533–2536. doi: 10.1073/pnas.77.5.2533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hong S. L., Levine L. Inhibition of arachidonic acid release from cells as the biochemical action of anti-inflammatory corticosteroids. Proc Natl Acad Sci U S A. 1976 May;73(5):1730–1734. doi: 10.1073/pnas.73.5.1730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. King S. J., Miller H. R. Anaphylactic release of mucosal mast cell protease and its relationship to gut permeability in Nippostrongylus-primed rats. Immunology. 1984 Apr;51(4):653–660. [PMC free article] [PubMed] [Google Scholar]
  14. Marquardt D. L., Wasserman S. I. Modulation of rat serosal mast cell biochemistry by in vivo dexamethasone administration. J Immunol. 1983 Aug;131(2):934–939. [PubMed] [Google Scholar]
  15. Miller H. R., Woodbury R. G., Huntley J. F., Newlands G. Systemic release of mucosal mast-cell protease in primed rats challenged with Nippostrongylus brasiliensis. Immunology. 1983 Jul;49(3):471–479. [PMC free article] [PubMed] [Google Scholar]
  16. Nawa Y., Miller H. R., Hall E., Jarrett E. E. Adoptive transfer of total and parasite-specific IgE responses in rats infected with Nippostrongylus brasiliensis. Immunology. 1981 Sep;44(1):119–123. [PMC free article] [PubMed] [Google Scholar]
  17. Nawa Y., Miller H. R. Protection against Nippostrongylus brasiliensis by adoptive immunization with immune thoracic duct lymphocytes. Cell Immunol. 1978 Apr;37(1):51–60. doi: 10.1016/0008-8749(78)90173-9. [DOI] [PubMed] [Google Scholar]
  18. Ogilvie B. M. Reagin-like antibodies in rats infected with the nematode parasite Nippostrongylus brasiliensis. Immunology. 1967 Feb;12(2):113–131. [PMC free article] [PubMed] [Google Scholar]
  19. Olson C. E., Schiller E. L. Strongyloides ratti infections in rats. II. Effects of cortisone treatment. Am J Trop Med Hyg. 1978 May;27(3):527–531. doi: 10.4269/ajtmh.1978.27.527. [DOI] [PubMed] [Google Scholar]
  20. SANYAL R. K., WEST G. B. Anaphylactic shock in the albino rat. J Physiol. 1958 Aug 6;142(3):571–584. doi: 10.1113/jphysiol.1958.sp006037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strobel S., Miller H. R., Ferguson A. Human intestinal mucosal mast cells: evaluation of fixation and staining techniques. J Clin Pathol. 1981 Aug;34(8):851–858. doi: 10.1136/jcp.34.8.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Urquhart G. M., Mulligan W., Eadie R. M., Jennings F. W. Immunological studies on Nippostrongylus brasiliensis infection in the rat: the role of local anaphylaxis. Exp Parasitol. 1965 Oct;17(2):210–217. doi: 10.1016/0014-4894(65)90023-8. [DOI] [PubMed] [Google Scholar]
  23. Woodbury R. G., Gruzenski G. M., Lagunoff D. Immunofluorescent localization of a serine protease in rat small intestine. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2785–2789. doi: 10.1073/pnas.75.6.2785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Woodbury R. G., Miller H. R. Quantitative analysis of mucosal mast cell protease in the intestines of Nippostrongylus-infected rats. Immunology. 1982 Jul;46(3):487–495. [PMC free article] [PubMed] [Google Scholar]
  25. Woodbury R. G., Neurath H. Purification of an atypical mast cell protease and its levels in developing rats. Biochemistry. 1978 Oct 3;17(20):4298–4304. doi: 10.1021/bi00613a029. [DOI] [PubMed] [Google Scholar]
  26. Woodbury R. G., Neurath H. Structure, specificity and localization of the serine proteases of connective tissue. FEBS Lett. 1980 Jun 2;114(2):189–196. doi: 10.1016/0014-5793(80)81112-4. [DOI] [PubMed] [Google Scholar]

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