Abstract
Three mouse monoclonal IgG3 antibodies, 2B2, IF4, and MG-21, recognize a GD3 ganglioside antigen that is expressed at the cell surface of most human melanomas. All three antibodies mediate antibody-dependent cellular cytotoxicity (ADCC) in vitro when tested with human lymphocytes as effector cells in a 2-hr or 4-hr 51Cr-release test, and one antibody, MG-21, also gives strong complement-dependent cytotoxicity with human serum. Antibody 2B2, which gives ADCC also in the presence of mouse lymphocytes, inhibited the outgrowth of a human melanoma in nude mice, but antibody IF4, which showed no ADCC with mouse lymphocyte effectors, did not.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown J. P., Nishiyama K., Hellström I., Hellström K. E. Structural characterization of human melanoma-associated antigen p97 with monoclonal antibodies. J Immunol. 1981 Aug;127(2):539–546. [PubMed] [Google Scholar]
- Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
- Dippold W. G., Knuth A., Meyer zum Büschenfelde K. H. Inhibition of human melanoma cell growth in vitro by monoclonal anti-GD3-ganglioside antibody. Cancer Res. 1984 Feb;44(2):806–810. [PubMed] [Google Scholar]
- Dippold W. G., Lloyd K. O., Li L. T., Ikeda H., Oettgen H. F., Old L. J. Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6114–6118. doi: 10.1073/pnas.77.10.6114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garrigues H. J., Tilgen W., Hellstróm I., Franke W., Hellström K. E. Detection of a human melanoma-associated antigen, p97, in histological sections of primary human melanomas. Int J Cancer. 1982 May 15;29(5):511–515. doi: 10.1002/ijc.2910290505. [DOI] [PubMed] [Google Scholar]
- Hellström I., Garrigues H. J., Cabasco L., Mosely G. H., Brown J. P., Hellström K. E. Studies of a high molecular weight human melanoma-associated antigen. J Immunol. 1983 Mar;130(3):1467–1472. [PubMed] [Google Scholar]
- Hellström I., Hellström K. E., Yeh M. Y. Lymphocyte-dependent antibodies to antigen 3.1, a cell-surface antigen expressed by a subgroup of human melanomas. Int J Cancer. 1981 Mar 15;27(3):281–285. doi: 10.1002/ijc.2910270305. [DOI] [PubMed] [Google Scholar]
- Nudelman E., Hakomori S., Kannagi R., Levery S., Yeh M. Y., Hellström K. E., Hellström I. Characterization of a human melanoma-associated ganglioside antigen defined by a monoclonal antibody, 4.2. J Biol Chem. 1982 Nov 10;257(21):12752–12756. [PubMed] [Google Scholar]
- Pukel C. S., Lloyd K. O., Travassos L. R., Dippold W. G., Oettgen H. F., Old L. J. GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody. J Exp Med. 1982 Apr 1;155(4):1133–1147. doi: 10.1084/jem.155.4.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schulz G., Bumol T. F., Reisfeld R. A. Monoclonal antibody-directed effector cells selectively lyse human melanoma cells in vitro and in vivo. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5407–5411. doi: 10.1073/pnas.80.17.5407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodbury R. G., Brown J. P., Yeh M. Y., Hellström I., Hellström K. E. Identification of a cell surface protein, p97, in human melanomas and certain other neoplasms. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2183–2187. doi: 10.1073/pnas.77.4.2183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yeh M. Y., Hellström I., Abe K., Hakomori S., Hellström K. E. A cell-surface antigen which is present in the ganglioside fraction and shared by human melanomas. Int J Cancer. 1982 Mar 15;29(3):269–275. doi: 10.1002/ijc.2910290308. [DOI] [PubMed] [Google Scholar]