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. 1985 Mar;82(5):1503–1507. doi: 10.1073/pnas.82.5.1503

Characterization of a Q subregion gene in the murine major histocompatibility complex.

L Flaherty, K DiBiase, M A Lynes, J G Seidman, O Weinberger, E M Rinchik
PMCID: PMC397291  PMID: 2983348

Abstract

We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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