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. 1985 Mar;82(5):1508–1512. doi: 10.1073/pnas.82.5.1508

Analysis of an evolutionarily conserved antigenic site on mammalian cytochrome c using synthetic peptides.

R Jemmerson, P R Morrow, N R Klinman, Y Paterson
PMCID: PMC397292  PMID: 2579398

Abstract

Two synthetic peptides inclusive of the NH2-terminal N-acetyl-Gly-Asp-Val-Glu tetrapeptide of mammalian cytochrome c (cyt c) were used in this study to address the question of whether mammals can respond immunologically to an evolutionarily conserved region of a protein. These peptides were assessed for their capacity (i) to act as immunogens for the production of anti-self cyt c antisera and (ii) to bind rabbit anti-rodent cyt c antibody. The findings from these studies indicate the existence of an immunogenic determinant in an evolutionarily conserved region of cyt c that contains residues 1-4. This determinant can induce anti-self cyt c antibodies whether presented as a peptide on a carrier protein or in the context of the intact molecule as polymerized mammalian cyt c.

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Selected References

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