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. 1985 Mar;82(5):1531–1535. doi: 10.1073/pnas.82.5.1531

Characterization of a protein, released by the T47D cell line, immunologically related to the major envelope protein of mouse mammary tumor virus.

N Segev, A Hizi, F Kirenberg, I Keydar
PMCID: PMC397297  PMID: 2983349

Abstract

The T47D human mammary adenocarcinoma cell line in vitro releases viral particles as well as soluble proteins. Both fractions were shown to contain antigens that immunologically crossreact with the major glycoprotein (gp52) of mouse mammary tumor virus. The crossreacting antigens are located on polypeptides with apparent molecular weights of about 68,000 and 60,000. The larger one is present in viral particles whereas both are found in the soluble fraction. Both proteins are glycosylated. The human tissue culture proteins differ from gp52 not only in molecular weight but also in charge heterogenity and in polypeptide profiles obtained after partial proteolysis. The results suggest that there is a restricted similarity between MMTV gp52 and the immunologically related T47D proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Callahan R., Drohan W., Tronick S., Schlom J. Detection and cloning of human DNA sequences related to the mouse mammary tumor virus genome. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5503–5507. doi: 10.1073/pnas.79.18.5503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dion A. S., Farwell D. C., Pomenti A. A., Girardi A. J. A human protein related to the major envelope protein of murine mammary tumor virus: identification and characterization. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1301–1305. doi: 10.1073/pnas.77.3.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hizi A., McCrae M. A., Joklik W. K. Studies on the amino acid sequence content of proteins specified by the gag and pol genes of avian sarcoma virus B77. Virology. 1978 Aug;89(1):272–284. doi: 10.1016/0042-6822(78)90059-4. [DOI] [PubMed] [Google Scholar]
  5. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  6. Keydar I., Chen L., Karby S., Weiss F. R., Delarea J., Radu M., Chaitcik S., Brenner H. J. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer. 1979 May;15(5):659–670. doi: 10.1016/0014-2964(79)90139-7. [DOI] [PubMed] [Google Scholar]
  7. Keydar I., Mesa-Tejada R., Ramanarayanan M., Ohno T., Fenoglio C., Hu R., Spiegelman S. Detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1524–1528. doi: 10.1073/pnas.75.3.1524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keydar I., Ohno T., Nayak R., Sweet R., Simoni F., Weiss F., Karby S., Mesa-Tejada R., Spiegelman S. Properties of retrovirus-like particles produced by a human breast carcinoma cell line: immunological relationship with mouse mammary tumor virus proteins. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4188–4192. doi: 10.1073/pnas.81.13.4188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keydar I., Selzer G., Chaitchik S., Hareuveni M., Karby S., Hizi A. A viral antigen as a marker for the prognosis of human breast cancer. Eur J Cancer Clin Oncol. 1982 Dec;18(12):1321–1328. doi: 10.1016/0277-5379(82)90136-5. [DOI] [PubMed] [Google Scholar]
  10. May F. E., Westley B. R., Rochefort H., Buetti E., Diggelmann H. Mouse mammary tumour virus related sequences are present in human DNA. Nucleic Acids Res. 1983 Jun 25;11(12):4127–4139. doi: 10.1093/nar/11.12.4127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mesa-Tejada R., Keydar I., Ramanarayanan M., Ohno T., Fenoglio C., Spiegelman S. Immunohistochemical detection of a cross-reacting virus antigen in mouse mammary tumors and human breast carcinomas. J Histochem Cytochem. 1978 Jul;26(7):532–541. doi: 10.1177/26.7.80418. [DOI] [PubMed] [Google Scholar]
  12. Moore D. H., Long C. A., Vaidya A. B., Sheffield J. B., Dion A. S., Lasfargues E. Y. Mammary tumor viruses. Adv Cancer Res. 1979;29:347–418. doi: 10.1016/s0065-230x(08)60850-7. [DOI] [PubMed] [Google Scholar]
  13. Nusse R., Janssen H., de Vries L., Michalides R. Analysis of secondary modifications of mouse mammary tumor virus proteins by two-dimensional gel electrophoresis. J Virol. 1980 Aug;35(2):340–348. doi: 10.1128/jvi.35.2.340-348.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  15. Ohno T., Mesa-Tejada R., Keydar I., Ramanarayanan M., Bausch J., Spiegelman S. Human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1979 May;76(5):2460–2464. doi: 10.1073/pnas.76.5.2460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ritzi E., Baldi A., Spiegelman S. The purification of a gs antigen of the murine mammary tumor virus and its quantitation by radioimmunoassay. Virology. 1976 Nov;75(1):188–197. doi: 10.1016/0042-6822(76)90017-9. [DOI] [PubMed] [Google Scholar]
  17. Yang N. S., McGrath C. M., Furmanski P. Presence of a mouse mammary tumor virus-related antigen in human breast carcinoma cells and its absence from normal mammary epithelial cells. J Natl Cancer Inst. 1978 Nov;61(5):1205–1208. doi: 10.1093/jnci/61.5.1205. [DOI] [PubMed] [Google Scholar]

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