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. 1985 Mar;82(6):1653–1657. doi: 10.1073/pnas.82.6.1653

Cloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene.

M C Graves, G T Mullenbach, J C Rabinowitz
PMCID: PMC397330  PMID: 3856844

Abstract

We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain greater than 6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobase-pair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.

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Selected References

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  1. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  2. Duggleby C. J., Jones S. A. Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli. Nucleic Acids Res. 1983 May 25;11(10):3065–3076. doi: 10.1093/nar/11.10.3065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gergen J. P., Stern R. H., Wensink P. C. Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 1979 Dec 20;7(8):2115–2136. doi: 10.1093/nar/7.8.2115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Himes R. H., Stallcup M. R., Rabinowitz J. C. Translation of synthetic and endogenous messenger ribonucleic acid in vitro by ribosomes and polyribosomes from Clostridium pasteurianum. J Bacteriol. 1972 Dec;112(3):1057–1069. doi: 10.1128/jb.112.3.1057-1069.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Karube I., Urano N., Yamada T., Hirochika H., Sakaguchi K. Cloning and expression of the hydrogenase gene from Clostridium butyricum in Escherichia coli. FEBS Lett. 1983 Jul 11;158(1):119–122. doi: 10.1016/0014-5793(83)80689-9. [DOI] [PubMed] [Google Scholar]
  7. Klein A., Schnorr M. Genome complexity of methanogenic bacteria. J Bacteriol. 1984 May;158(2):628–631. doi: 10.1128/jb.158.2.628-631.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Liao H. H., Rabinowitz J. C. Clostridial apoferredoxin messenger ribonucleic acid. Assay and partial purification. Biochim Biophys Acta. 1980 Jul 29;608(2):301–314. doi: 10.1016/0005-2787(80)90176-8. [DOI] [PubMed] [Google Scholar]
  9. Losick R., Pero J. Cascades of Sigma factors. Cell. 1981 Sep;25(3):582–584. doi: 10.1016/0092-8674(81)90164-1. [DOI] [PubMed] [Google Scholar]
  10. Makaroff C. A., Zalkin H., Switzer R. L., Vollmer S. J. Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and properties of the plasmid-encoded enzyme. J Biol Chem. 1983 Sep 10;258(17):10586–10593. [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  12. McLaughlin J. R., Murray C. L., Rabinowitz J. C. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. J Biol Chem. 1981 Nov 10;256(21):11283–11291. [PubMed] [Google Scholar]
  13. Moran C. P., Jr, Lang N., LeGrice S. F., Lee G., Stephens M., Sonenshein A. L., Pero J., Losick R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982;186(3):339–346. doi: 10.1007/BF00729452. [DOI] [PubMed] [Google Scholar]
  14. Murphy N., McConnell D. J., Cantwell B. A. The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase. Nucleic Acids Res. 1984 Jul 11;12(13):5355–5367. doi: 10.1093/nar/12.13.5355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murray C. L., Rabinowitz J. C. Nucleotide sequences of transcription and translation initiation regions in Bacillus phage phi 29 early genes. J Biol Chem. 1982 Jan 25;257(2):1053–1062. [PubMed] [Google Scholar]
  16. Murray C. L., Rabinowitz J. C. RNA polymerase from Clostridium acidi-urici. Characterization of a naturally occurring rifampicin-resistant bacterial enzyme. J Biol Chem. 1981 May 25;256(10):5153–5161. [PubMed] [Google Scholar]
  17. Neugebauer K., Sprengel R., Schaller H. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium. Nucleic Acids Res. 1981 Jun 11;9(11):2577–2588. doi: 10.1093/nar/9.11.2577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rabinowitz J. Preparation and properties of clostridial ferredoxins. Methods Enzymol. 1972;24:431–446. doi: 10.1016/0076-6879(72)24089-7. [DOI] [PubMed] [Google Scholar]
  19. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  20. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  21. Sieker L. C., Adman E., Jensen L. H. Structure of the Fe-S complex in a bacterial ferredoxin. Nature. 1972 Jan 7;235(5332):40–42. doi: 10.1038/235040a0. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Stallcup M. R., Rabinowitz J. C. Initiation of protein synthesis in vitro by a clostridial system. I. Specificity in the translation of natural messenger ribonucleic acids. J Biol Chem. 1973 May 10;248(9):3209–3215. [PubMed] [Google Scholar]
  24. Stallcup M. R., Sharrock W. J., Rabinowitz J. C. Ribsome and messenger specificity in protein synthesis by bacteria. Biochem Biophys Res Commun. 1974 May 7;58(1):92–98. doi: 10.1016/0006-291x(74)90895-x. [DOI] [PubMed] [Google Scholar]
  25. Stormo G. D., Schneider T. D., Gold L. M. Characterization of translational initiation sites in E. coli. Nucleic Acids Res. 1982 May 11;10(9):2971–2996. doi: 10.1093/nar/10.9.2971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. TANAKA M., NAKASHIMA T., MOWER H. F., YASUNOBU K. T. THE C- AND N-TERMINAL AMINO ACID SEQUENCES OF CLOSTRIDIUM PASTEURIANUM FERREDOXIN. Arch Biochem Biophys. 1964 Jun;105:570–574. doi: 10.1016/0003-9861(64)90052-9. [DOI] [PubMed] [Google Scholar]
  27. TONOMURA B., MALKIN R., RABINOWITZ J. C. DEOXYRIBONUCLEIC ACID BASE COMPOSITION OF CLOSTRIDIAL SPECIES. J Bacteriol. 1965 May;89:1438–1439. doi: 10.1128/jb.89.5.1438-1439.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tsao S. G., Brunk C. F., Pearlman R. E. Hybridization of nucleic acids directly in agarose gels. Anal Biochem. 1983 Jun;131(2):365–372. doi: 10.1016/0003-2697(83)90185-9. [DOI] [PubMed] [Google Scholar]
  29. Urdea M. S., Merryweather J. P., Mullenbach G. T., Coit D., Heberlein U., Valenzuela P., Barr P. J. Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7461–7465. doi: 10.1073/pnas.80.24.7461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wells J. A., Ferrari E., Henner D. J., Estell D. A., Chen E. Y. Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. Nucleic Acids Res. 1983 Nov 25;11(22):7911–7925. doi: 10.1093/nar/11.22.7911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wiggs J. L., Bush J. W., Chamberlin M. J. Utilization of promoter and terminator sites on bacteriophage T7 DNA by RNA polymerases from a variety of bacterial orders. Cell. 1979 Jan;16(1):97–109. doi: 10.1016/0092-8674(79)90191-0. [DOI] [PubMed] [Google Scholar]

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