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. 1985 Mar;82(6):1658–1662. doi: 10.1073/pnas.82.6.1658

Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.

D Mornet, K Ue, M F Morales
PMCID: PMC397331  PMID: 3856845

Abstract

A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain and seems to place the dibromobimane near the ATP-perturbable tryptophan.

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Selected References

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