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. 1985 Mar;82(6):1781–1784. doi: 10.1073/pnas.82.6.1781

beta-Adrenergic agonist activity of a monoclonal anti-idiotypic antibody.

J G Guillet, S V Kaveri, O Durieu, C Delavier, J Hoebeke, A D Strosberg
PMCID: PMC397356  PMID: 2858851

Abstract

Hybridoma cells bearing monoclonal antibody against the beta-adrenergic ligand alprenolol were used as an immunogen to raise monoclonal anti-idiotypic antibodies. Of six anti-idiotypic antibodies, which inhibit ligand binding, three were able to recognize beta-adrenergic receptors. One of them, mAb2B4, an IgM that could be amplified into ascites, binds to the beta-adrenergic catecholamine receptors of intact epidermoid A431 cells and precipitates receptors solubilized from plasma membranes by digitonin. This antibody identifies the beta 2-adrenergic receptor of A431 cells as a single 55-kDa protein and stimulates adenylate cyclase activity. This stimulation is inhibited by the beta-adrenergic antagonist propranolol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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