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. 1985 Apr;82(7):2072–2076. doi: 10.1073/pnas.82.7.2072

Transposon-like sequences in extrachromosomal circular DNA from mouse thymocytes.

S Fujimoto, T Tsuda, M Toda, H Yamagishi
PMCID: PMC397494  PMID: 2984679

Abstract

Small polydisperse circular (spc) DNA was isolated from mouse thymocytes and cloned into the HindIII site of lambda vector Charon 7. Fifty-six recombinants from this spc DNA library were analyzed. R repeats, which were originally found near immunoglobulin genes, were enriched in spc DNA clones relative to their representation in the chromosome. In one clone, the R sequence was linked to Bam and MIF sequences and the contiguous arrangement was truncated from both ends. In another clone, composite Bam/R and R repeats existed as a pair in inverted repeat orientation. Truncation occurred from the 5' side without affecting the 3' ends. In both clones, short direct repeats flanked the repeated sequences. The possible role of R sequences in transposition and circular formation is discussed.

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Selected References

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