Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Apr;82(7):2148–2152. doi: 10.1073/pnas.82.7.2148

In vivo solvent-suppressed localized hydrogen nuclear magnetic resonance spectroscopy: a window to metabolism?

P A Bottomley, W A Edelstein, T H Foster, W A Adams
PMCID: PMC397510  PMID: 3856889

Abstract

Solvent-suppression NMR techniques are combined with a pulsed magnetic field gradient and surface coil detection method of spatial localization. The result is a technique that enables observation of metabolites in the hydrogen (1H) NMR chemical-shift spectra from preselected disk-shaped volumes of biological tissue in vivo. Localized spectra are recorded from the normal human brain and forearm and from a dog in acquisition periods of 2 s using a 1.5-T imaging/spectroscopy system. This is several hundred-fold faster than acquiring similar state-of-the-art 31P NMR spectra of brain metabolites in vivo. Spectroscopy experiments are followed by conventional surface coil imaging sequences to precisely define the selected volume. Contamination of spectra by lipid resonances is a problem.

Full text

PDF
2148

Images in this article

2150Image
on p.2150
2150Image
on p.2150
2151Image
on p.2151

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackerman J. J., Grove T. H., Wong G. G., Gadian D. G., Radda G. K. Mapping of metabolites in whole animals by 31P NMR using surface coils. Nature. 1980 Jan 10;283(5743):167–170. doi: 10.1038/283167a0. [DOI] [PubMed] [Google Scholar]
  2. Behar K. L., Rothman D. L., Shulman R. G., Petroff O. A., Prichard J. W. Detection of cerebral lactate in vivo during hypoxemia by 1H NMR at relatively low field strengths (1.9 T). Proc Natl Acad Sci U S A. 1984 Apr;81(8):2517–2519. doi: 10.1073/pnas.81.8.2517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Behar K. L., den Hollander J. A., Stromski M. E., Ogino T., Shulman R. G., Petroff O. A., Prichard J. W. High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4945–4948. doi: 10.1073/pnas.80.16.4945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bottomley P. A., Edelstein W. A., Hart H. R., Schenck J. F., Smith L. S. Spatial localization in 31P and 13C NMR spectroscopy in vivo using surface coils. Magn Reson Med. 1984 Sep;1(3):410–413. doi: 10.1002/mrm.1910010311. [DOI] [PubMed] [Google Scholar]
  5. Bottomley P. A., Foster T. H., Leue W. M. Chemical imaging of the brain by NMR. Lancet. 1984 May 19;1(8386):1120–1120. doi: 10.1016/s0140-6736(84)92529-7. [DOI] [PubMed] [Google Scholar]
  6. Bottomley P. A., Foster T. H., Leue W. M. In vivo nuclear magnetic resonance chemical shift imaging by selective irradiation. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6856–6860. doi: 10.1073/pnas.81.21.6856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bottomley P. A., Hart H. R., Edelstein W. A., Schenck J. F., Smith L. S., Leue W. M., Mueller O. M., Redington R. W. NMR imaging/spectroscopy system to study both anatomy and metabolism. Lancet. 1983 Jul 30;2(8344):273–274. doi: 10.1016/s0140-6736(83)90250-7. [DOI] [PubMed] [Google Scholar]
  8. Bottomley P. A., Hart H. R., Jr, Edelstein W. A., Schenck J. F., Smith L. S., Leue W. M., Mueller O. M., Redington R. W. Anatomy and metabolism of the normal human brain studied by magnetic resonance at 1.5 Tesla. Radiology. 1984 Feb;150(2):441–446. doi: 10.1148/radiology.150.2.6691099. [DOI] [PubMed] [Google Scholar]
  9. Bottomley P. A., Kogure K., Namon R., Alonso O. F. Cerebral energy metabolism in rats studied by phosphorus nuclear magnetic resonance using surface coils. Magn Reson Imaging. 1982;1(2):81–85. doi: 10.1016/0730-725x(82)90223-5. [DOI] [PubMed] [Google Scholar]
  10. Cady E. B., Costello A. M., Dawson M. J., Delpy D. T., Hope P. L., Reynolds E. O., Tofts P. S., Wilkie D. R. Non-invasive investigation of cerebral metabolism in newborn infants by phosphorus nuclear magnetic resonance spectroscopy. Lancet. 1983 May 14;1(8333):1059–1062. doi: 10.1016/s0140-6736(83)91906-2. [DOI] [PubMed] [Google Scholar]
  11. Decorps M., Lebas J. F., Leviel J. L., Confort S., Remy C., Benabid A. L. Analysis of brain metabolism changes induced by acute potassium cyanide intoxication by 31P NMR in vivo using chronically implanted surface coils. FEBS Lett. 1984 Mar 12;168(1):1–6. doi: 10.1016/0014-5793(84)80195-7. [DOI] [PubMed] [Google Scholar]
  12. Edelstein W. A., Bottomley P. A., Pfeifer L. M. A signal-to-noise calibration procedure for NMR imaging systems. Med Phys. 1984 Mar-Apr;11(2):180–185. doi: 10.1118/1.595484. [DOI] [PubMed] [Google Scholar]
  13. Edelstein W. A., Schenck J. F., Hart H. R., Hardy C. J., Foster T. H., Bottomley P. A. Surface coil magnetic resonance imaging. JAMA. 1985 Feb 8;253(6):828–828. [PubMed] [Google Scholar]
  14. Edwards R. H., Dawson M. J., Wilkie D. R., Gordon R. E., Shaw D. Clinical use of nuclear magnetic resonance in the investigation of myopathy. Lancet. 1982 Mar 27;1(8274):725–731. doi: 10.1016/s0140-6736(82)92635-6. [DOI] [PubMed] [Google Scholar]
  15. Evanochko W. T., Ng T. C., Lilly M. B., Lawson A. J., Corbett T. H., Durant J. R., Glickson J. D. In vivo 31P NMR study of the metabolism of murine mammary 16/C adenocarcinoma and its response to chemotherapy, x-radiation, and hyperthermia. Proc Natl Acad Sci U S A. 1983 Jan;80(2):334–338. doi: 10.1073/pnas.80.2.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gordon R. E., Hanley P. E., Shaw D., Gadian D. G., Radda G. K., Styles P., Bore P. J., Chan L. Localization of metabolites in animals using 31P topical magnetic resonance. Nature. 1980 Oct 23;287(5784):736–738. doi: 10.1038/287736a0. [DOI] [PubMed] [Google Scholar]
  17. Grove T. H., Ackerman J. J., Radda G. K., Bore P. J. Analysis of rat heart in vivo by phosphorus nuclear magnetic resonance. Proc Natl Acad Sci U S A. 1980 Jan;77(1):299–302. doi: 10.1073/pnas.77.1.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Koretsky A. P., Wang S., Murphy-Boesch J., Klein M. P., James T. L., Weiner M. W. 31P NMR spectroscopy of rat organs, in situ, using chronically implanted radiofrequency coils. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7491–7495. doi: 10.1073/pnas.80.24.7491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matthews P. M., Williams S. R., Seymour A. M., Schwartz A., Dube G., Gadian D. G., Radda G. K. A 31P-NMR study of some metabolic and functional effects of the inotropic agents epinephrine and ouabain, and the ionophore R02-2985 (X537A) in the isolated, perfused rat heart. Biochim Biophys Acta. 1982 Apr 29;720(2):163–171. doi: 10.1016/0167-4889(82)90008-8. [DOI] [PubMed] [Google Scholar]
  20. Nunnally R. L., Bottomley P. A. Assessment of pharmacological treatment of myocardial infarction by phosphorus-31 NMR with surface coils. Science. 1981 Jan 9;211(4478):177–180. doi: 10.1126/science.7444460. [DOI] [PubMed] [Google Scholar]
  21. Nunnally R. L., Hollis D. P. Adenosine triphosphate compartmentation in living hearts: a phosphorus nuclear magnetic resonance saturation transfer study. Biochemistry. 1979 Aug 7;18(16):3642–3646. doi: 10.1021/bi00583a032. [DOI] [PubMed] [Google Scholar]
  22. Prichard J. W., Alger J. R., Behar K. L., Petroff O. A., Shulman R. G. Cerebral metabolic studies in vivo by 31P NMR. Proc Natl Acad Sci U S A. 1983 May;80(9):2748–2751. doi: 10.1073/pnas.80.9.2748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Radda G. K., Bore P. J., Gadian D. G., Ross B. D., Styles P., Taylor D. J., Morgan-Hughes J. 31P NMR examination of two patients with NADH-CoQ reductase deficiency. Nature. 1982 Feb 18;295(5850):608–609. doi: 10.1038/295608a0. [DOI] [PubMed] [Google Scholar]
  24. Ross B. D., Radda G. K., Gadian D. G., Rocker G., Esiri M., Falconer-Smith J. Examination of a case of suspected McArdle's syndrome by 31P nuclear magnetic resonance. N Engl J Med. 1981 May 28;304(22):1338–1342. doi: 10.1056/NEJM198105283042206. [DOI] [PubMed] [Google Scholar]
  25. Shoubridge E. A., Briggs R. W., Radda G. K. 31p NMR saturation transfer measurements of the steady state rates of creatine kinase and ATP synthetase in the rat brain. FEBS Lett. 1982 Apr 19;140(2):289–292. doi: 10.1016/0014-5793(82)80916-2. [DOI] [PubMed] [Google Scholar]
  26. Thulborn K. R., du Boulay G. H., Duchen L. W., Radda G. A 31P nuclear magnetic resonance in vivo study of cerebral ischaemia in the gerbil. J Cereb Blood Flow Metab. 1982 Sep;2(3):299–306. doi: 10.1038/jcbfm.1982.31. [DOI] [PubMed] [Google Scholar]
  27. Ugurbil K., Petein M., Maidan R., Michurski S., Cohn J. N., From A. H. High resolution proton NMR studies of perfused rat hearts. FEBS Lett. 1984 Feb 13;167(1):73–78. doi: 10.1016/0014-5793(84)80835-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES