Abstract
Bacterial threads of up to 1 m in length have been produced from filaments of separation-suppressed mutants of Bacillus subtilis. Individual threads may contain 20,000 cellular filaments in parallel alignment. The tensile properties of bacterial threads have been examined by using conventional textile engineering techniques. The kinetics of elongation at constant load are indicative of a viscoelastic material. Both Young's modulus and breaking stress are highly dependent upon relative humidity. By extrapolation to 100% relative humidity, it appears that cell walls may be able to bear only internal osmotic pressures of about 2 atmospheres (2.03 X 105(5) Pa) in living cells. Similarly, the strength of wall material limits the amount of cell-surface charge permissible to only a small fraction of that known to be carried by the negatively charged wall polymers.
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Selected References
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- Breakefield X. O., Landman O. E. Temperature-sensitive divisionless mutant of Bacillus subtilis defective in the initiation of septation. J Bacteriol. 1973 Feb;113(2):985–998. doi: 10.1128/jb.113.2.985-998.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carstensen E. L., Marquis R. E. Passive electrical properties of microorganisms. 3. Conductivity of isolated bacterial cell walls. Biophys J. 1968 May;8(5):536–548. doi: 10.1016/s0006-3495(68)86506-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Corner T. R., Marquis R. E. Why do bacterial protoplasts burst in hypotonic solutions? Biochim Biophys Acta. 1969;183(3):544–558. doi: 10.1016/0005-2736(69)90168-0. [DOI] [PubMed] [Google Scholar]
- Doyle R. J., Matthews T. H., Streips U. N. Chemical basis for selectivity of metal ions by the Bacillus subtilis cell wall. J Bacteriol. 1980 Jul;143(1):471–480. doi: 10.1128/jb.143.1.471-480.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KNAYSI G., HILLIER J., FABRICANT C. The cytology of an avian strain of Mycobacterium tuberculosis studied with the electron and light microscopes. J Bacteriol. 1950 Oct;60(4):423–447. doi: 10.1128/jb.60.4.423-447.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koch A. L. The surface stress theory of microbial morphogenesis. Adv Microb Physiol. 1983;24:301–366. doi: 10.1016/s0065-2911(08)60388-4. [DOI] [PubMed] [Google Scholar]
- Marquis R. E., Carstensen E. L. Electric conductivity and internal osmolality of intact bacterial cells. J Bacteriol. 1973 Mar;113(3):1198–1206. doi: 10.1128/jb.113.3.1198-1206.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marquis R. E. Salt-induced contraction of bacterial cell walls. J Bacteriol. 1968 Mar;95(3):775–781. doi: 10.1128/jb.95.3.775-781.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mendelson N. H., Favre D., Thwaites J. J. Twisted states of Bacillus subtilis macrofibers reflect structural states of the cell wall. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3562–3566. doi: 10.1073/pnas.81.11.3562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mendelson N. H. Helical Bacillus subtilis macrofibers: morphogenesis of a bacterial multicellular macroorganism. Proc Natl Acad Sci U S A. 1978 May;75(5):2478–2482. doi: 10.1073/pnas.75.5.2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mendelson N. H. Helical growth of Bacillus subtilis: a new model of cell growth. Proc Natl Acad Sci U S A. 1976 May;73(5):1740–1744. doi: 10.1073/pnas.73.5.1740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mendelson N. H., Karamata D. Inversion of helix orientation in Bacillus subtilis macrofibers. J Bacteriol. 1982 Jul;151(1):450–454. doi: 10.1128/jb.151.1.450-454.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ou L. T., Marquis R. E. Electromechanical interactions in cell walls of gram-positive cocci. J Bacteriol. 1970 Jan;101(1):92–101. doi: 10.1128/jb.101.1.92-101.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Von Hippel P. H., Hamabata A. Model studies on the effects of neutral salts on the conformational stability of biological macromolecules. J Mechanochem Cell Motil. 1973 May;2(1):127–138. [PubMed] [Google Scholar]