Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 May;82(9):2657–2659. doi: 10.1073/pnas.82.9.2657

Detection in extracts of bovine brain of lipophilic complexes of sulfate esters of cholesterol and beta-sitosterol.

V V Prasad, E el-Maraghy, L Ponticorvo, S Lieberman
PMCID: PMC397623  PMID: 3857607

Abstract

Evidence indicating that there exist in bovine brains hitherto-unrecognized lipophilic conjugates of sterol sulfates is presented. These conjugates are soluble in nonpolar solvents and, when heated in methanol containing pyridine, yield polar sterol conjugates. These polar substances have the chromatographic mobility of sterol sulfates and are cleaved to free sterols when subjected to a solvolytic process known to be specific for sulfate esters. The brain sterols that have been identified in this way are cholesterol and beta-sitosterol.

Full text

PDF
2657

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURSTEIN S., LIEBERMAN S. Hydrolysis of ketosteroid hydrogen sulfates by solvolysis procedures. J Biol Chem. 1958 Aug;233(2):331–335. [PubMed] [Google Scholar]
  2. Benes P., Schirazi M., Oertel G. W. Steroid conjugates in plasma. XXIV. Formation of lipophil steroid sulfoconjugates from 7 alpha-3H-DHEA sulfate and carboxyl-14C diglyceride. Steroids Lipids Res. 1973;4(5):285–294. [PubMed] [Google Scholar]
  3. DRAYER N. M., LIEBERMAN S. ISOLATION OF CHOLESTEROL SULFATE FROM HUMAN BLOOD AND GALLSTONES. Biochem Biophys Res Commun. 1965 Jan 4;18:126–130. doi: 10.1016/0006-291x(65)90894-6. [DOI] [PubMed] [Google Scholar]
  4. Moser H. W., Moser A. B., Orr J. C. Preliminary observations on the occurrence of cholesterol sulfate in man. Biochim Biophys Acta. 1966 Feb 1;116(1):146–155. doi: 10.1016/0005-2760(66)90100-7. [DOI] [PubMed] [Google Scholar]
  5. OERTEL G. W. [On steroid conjugates in plasma. I. Isolation of sulfate-, phosphate- and lipoid-containing 17-ketosteroid conjugates in human blood plasma]. Biochem Z. 1961;334:431–440. [PubMed] [Google Scholar]
  6. OERTEL W., KAISER E. [On steroid conjugates in the plasma. IV. Chromatography of steroid conjugates on ion exchangers and use in the isolation of 17-ketosteroid conjugates from the peripheral plasma]. Biochem Z. 1962;336:10–19. [PubMed] [Google Scholar]
  7. ROBERTS K. D., BANDI L., CALVIN H. I., DRUCKER W. D., LIEBERMAN S. EVIDENCE THAT STEROID SULFATES SERVE AS BIOSYNTHETIC INTERMEDIATES. IV. CONVERSION OF CHOLESTEROL SULFATE IN VIVO TO URINARY C-19 AND C-21 STEROIDAL SULFATES. Biochemistry. 1964 Dec;3:1983–1988. doi: 10.1021/bi00900a034. [DOI] [PubMed] [Google Scholar]
  8. Salen G., Ahrens E. H., Jr, Grundy S. M. Metabolism of beta-sitosterol in man. J Clin Invest. 1970 May;49(5):952–967. doi: 10.1172/JCI106315. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES