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. 1985 May;82(10):3078–3081. doi: 10.1073/pnas.82.10.3078

Rapid "footprinting" on supercoiled DNA.

J D Gralla
PMCID: PMC397717  PMID: 3889902

Abstract

A DNase protection technique is described and applied to the interaction of three lac control proteins with supercoiled lac DNA. The technique uses end-labeled oligonucleotide primers to probe specific DNA regions as an alternative to protocols requiring restriction endonuclease cleavage or blotting. Thus DNA may be probed with high resolution in its native state. It is demonstrated that the introduction of supercoiling into DNA accelerates the rate of lac ps promoter binding by RNA polymerase but does not alter the positions at which polymerase, c-AMP-binding protein, or lac repressor bind to lac DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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