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. 2014 Mar 7;53(12):2032–2042. doi: 10.1021/bi500102w

Figure 1.

Figure 1

(A) Topology of the 100–243 construct of the KCNQ1 voltage-sensor domain (Q1-VSD), which spans helices S0–S4, as inferred on the basis of the results of this work. The sites for mutations linked to long QT syndrome and related disorders are colored pink, while sites for mutations linked to atrial fibrillation (AF) and related disorders are colored yellow. (B) Sequence alignment (by ClusterW2) of TM segments S2 and S4 of VSDs from various voltage-gated ion channels. (C) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel stained with Coomassie blue, showing that purified Q1-VSD runs as a monomer in SDS micelles, with an apparent molecular mass of 15 kDa.