. 2014 Feb 25;8(1):014110. doi: 10.1063/1.4866766

TABLE I.

Digital detection results based on single-molecule counting with different β-Gal concentrations.

Sample concentration (μM)	Dilution factor	Input concentration (fM)	Fraction of none-fluorescent droplets	λ_mes	Measured enzyme concentration in droplet (C_mes, fM)	RSD %		Average of measured sample concentration (μM)
0.89 $\pm$ 0.26	4.50 × 10⁻⁸	40	0.899 $\pm 0.006$	0.106 $\pm 0.007$	42.0 $\pm 2.86$	6.8	0.93 $\pm 0.06$	0.88 $\pm$ 0.02
	1.13 × 10⁻⁷	101	0.782 $\pm$ 0.010	0.246 $\pm$ 0.013	97.4 $\pm 5.42$	5.6	0.86 $\pm 0.05$
	2.25 × 10⁻⁷	200	0.616 $\pm$ 0.007	0.485 $\pm$ 0.011	192 $\pm 5.48$	2.9	0.85 $\pm$ 0.02
	3.38 × 10⁻⁷	301	0.472 $\pm$ 0.011	0.751 $\pm$ 0.023	297 $\pm 10.4$	3.5	0.88 $\pm$ 0.03
	4.50 × 10⁻⁷	401	0.366 $\pm$ 0.006	1.005 $\pm$ 0.016	398 $\pm 9.36$	2.4	0.88 $\pm$ 0.02
	9.00 × 10⁻⁷	801	0.118 $\pm$ 0.006	2.143 $\pm$ 0.051	848 $\pm 25.0$	3.0	0.94 $\pm$ 0.03
	1.35 × 10⁻⁶	1202	0.055 $\pm$ 0.006	2.913 $\pm 0.110$	1150 $\pm$ 47.8	4.2	0.85 $\pm$ 0.04