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. 1985 Jun;82(11):3557–3561. doi: 10.1073/pnas.82.11.3557

Tobacco chloroplast tRNALys(UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intron

Mamoru Sugita *, Kazuo Shinozaki †, Masahiro Sugiura †,‡
PMCID: PMC397824  PMID: 16593561

Abstract

The nucleotide sequence of a tRNALys(UUU) gene on tobacco (Nicotiana tabacum) chloroplast DNA has been determined. This gene is located 215 base pairs upstream from the gene for the 32,000-dalton thylakoid membrane protein on the same DNA strand and has a 2526-base-pair intron in the anticodon loop. The intron boundary sequence does not follow the G-U/A-G rule but is similar to those of tobacco chloroplast split genes for tRNAGly(UCC) and ribosomal proteins L2 and S12. The intron contains one major open reading frame of 509 codons. The codon usage in the open reading frame resembles those observed in the genes for tobacco chloroplast proteins so far analyzed. The primary transcript of this tRNA gene is 2.7 kilobases long.

Keywords: molecular cloning, DNA sequence, blot hybridization, precursor RNA, codon usage

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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