Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Jun;82(11):3741–3745. doi: 10.1073/pnas.82.11.3741

Deletion mapping of HLA and chromosome 6p genes.

F Levine, H Erlich, B Mach, R Leach, R White, D Pious
PMCID: PMC397863  PMID: 2987942

Abstract

We have analyzed a set of heterozygous mutants with deletions that encompass parts of HLA and surrounding regions of chromosome 6p. By a combination of Southern blotting, serologic, enzymatic, and cytogenetic analyses, we have ordered eight independent deletion break points into a sequence that divides chromosome 6p into six regions. The deletion mutants have been used in conjunction with the Southern blot technique to map HLA and other 6p gene probes into those regions. On the basis of these and other data we propose a genetic and physical map of HLA and surrounding regions of chromosome 6p. We find that for HLA probes, most of which hybridize with more than one gene, the multiple copies recognized by single probes map to single regions. Any chromosome 6p gene can now be regionally mapped by using these mutants.

Full text

PDF
3741

Images in this article

3742Image
on p.3742
3742Image
on p.3742
3742Image
on p.3742
3742Image
on p.3742
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3743Image
on p.3743
3744Image
on p.3744
3744Image
on p.3744

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auffray C., Ben-Nun A., Roux-Dosseto M., Germain R. N., Seidman J. G., Strominger J. L. Polymorphism and complexity of the human DC and murine I-A alpha chain genes. EMBO J. 1983;2(1):121–124. doi: 10.1002/j.1460-2075.1983.tb01392.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carroll M. C., Campbell R. D., Bentley D. R., Porter R. R. A molecular map of the human major histocompatibility complex class III region linking complement genes C4, C2 and factor B. Nature. 1984 Jan 19;307(5948):237–241. doi: 10.1038/307237a0. [DOI] [PubMed] [Google Scholar]
  3. Day C. E., Jones P. P. The gene encoding the Ia antigen-associated invariant chain (Ii) is not linked to the H-2 complex. Nature. 1983 Mar 10;302(5904):157–159. doi: 10.1038/302157a0. [DOI] [PubMed] [Google Scholar]
  4. Erlich H. A., Stetler D., Saiki R., Gladstone P., Pious D. Mapping of the genes encoding the HLA-DR alpha chain and the HLA-related antigens to a chromosome 6 deletion by using genomic blotting. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2300–2304. doi: 10.1073/pnas.80.8.2300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gladstone P., Fueresz L., Pious D. Gene dosage and gene expression in the HLA region: evidence from deletion variants. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1235–1239. doi: 10.1073/pnas.79.4.1235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gorski J., Rollini P., Long E., Mach B. Molecular organization of the HLA-SB region of the human major histocompatibility complex and evidence for two SB beta-chain genes. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3934–3938. doi: 10.1073/pnas.81.13.3934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kapuściński J., Skoczylas B. Simple and rapid fluorimetric method for DNA microassay. Anal Biochem. 1977 Nov;83(1):252–257. doi: 10.1016/0003-2697(77)90533-4. [DOI] [PubMed] [Google Scholar]
  8. Kaufman J. F., Auffray C., Korman A. J., Shackelford D. A., Strominger J. The class II molecules of the human and murine major histocompatibility complex. Cell. 1984 Jan;36(1):1–13. doi: 10.1016/0092-8674(84)90068-0. [DOI] [PubMed] [Google Scholar]
  9. Kavathas P., DeMars R., Bach F. H., Shaw S. SB: a new HLA-linked human histocompatibility gene defined using HLA-mutant cell lines. Nature. 1981 Oct 29;293(5835):747–749. doi: 10.1038/293747a0. [DOI] [PubMed] [Google Scholar]
  10. Kratzin H., Yang C. Y., Götz H., Pauly E., Kölbel S., Egert G., Thinnes F. P., Wernet P., Altevogt P., Hilschmann N. Primärstruktur menschlicher Histokompatibilitätsantigene der Klasse II. 1. Mitteilung: Aminosäuresequenz der N-terminalen 198 Reste der beta-Kette des HLA-Dw2,2;DR2,2-Alloantigens. Hoppe Seylers Z Physiol Chem. 1981 Dec;362(12):1665–1669. [PubMed] [Google Scholar]
  11. Long E. O., Gorski J., Mach B. Structural relationship of the SB beta-chain gene to HLA-D-region genes and murine I-region genes. Nature. 1984 Jul 19;310(5974):233–235. doi: 10.1038/310233a0. [DOI] [PubMed] [Google Scholar]
  12. Long E. O., Wake C. T., Gorski J., Mach B. Complete sequence of an HLA-dR beta chain deduced from a cDNA clone and identification of multiple non-allelic DR beta chain genes. EMBO J. 1983;2(3):389–394. doi: 10.1002/j.1460-2075.1983.tb01435.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Michelson A. M., Markham A. F., Orkin S. H. Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc Natl Acad Sci U S A. 1983 Jan;80(2):472–476. doi: 10.1073/pnas.80.2.472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roux-Dosseto M., Auffray C., Lillie J. W., Boss J. M., Cohen D., DeMars R., Mawas C., Seidman J. G., Strominger J. L. Genetic mapping of a human class II antigen beta-chain cDNA clone to the SB region of the HLA complex. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6036–6040. doi: 10.1073/pnas.80.19.6036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shaw S., Kavathas P., Pollack M. S., Charmot D., Mawas C. Family studies define a new histocompatibility locus, SB, between HLA-DR and GLO. Nature. 1981 Oct 29;293(5835):745–747. doi: 10.1038/293745a0. [DOI] [PubMed] [Google Scholar]
  16. Sood A. K., Pereira D., Weissman S. M. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A. 1981 Jan;78(1):616–620. doi: 10.1073/pnas.78.1.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spielman R. S., Lee J., Bodmer W. F., Bodmer J. G., Trowsdale J. Six HLA-D region alpha-chain genes on human chromosome 6: polymorphisms and associations of DC alpha-related sequences with DR types. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3461–3465. doi: 10.1073/pnas.81.11.3461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stetler D., Das H., Nunberg J. H., Saiki R., Sheng-Dong R., Mullis K. B., Weissman S. M., Erlich H. A. Isolation of a cDNA clone for the human HLA-DR antigen alpha chain by using a synthetic oligonucleotide as a hybridization probe. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5966–5970. doi: 10.1073/pnas.79.19.5966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Szabo P., Grzeschik K. H., Siniscalco M. A human autosomal phosphoglycerate kinase locus maps near the HLA cluster. Proc Natl Acad Sci U S A. 1984 May;81(10):3167–3169. doi: 10.1073/pnas.81.10.3167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wake C. T., Long E. O., Mach B. Allelic polymorphism and complexity of the genes for HLA-DR beta-chains--direct analysis by DNA-DNA hybridization. Nature. 1982 Nov 25;300(5890):372–374. doi: 10.1038/300372a0. [DOI] [PubMed] [Google Scholar]
  21. Weitkamp L. R., Lamm L. U. Report of the committee on the genetic constitution of chromosome 6. Oslo Conference (1981): Sixth International Workshop on Human Gene Mapping. Cytogenet Cell Genet. 1982;32(1-4):130–143. doi: 10.1159/000131693. [DOI] [PubMed] [Google Scholar]
  22. Whitehead A. S., Goldberger G., Woods D. E., Markham A. F., Colten H. R. Use of a cDNA clone for the fourth component of human complement (C4) for analysis of a genetic deficiency of C4 in guinea pig. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5387–5391. doi: 10.1073/pnas.80.17.5387. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES