(A) Cumulative copulation curve for males during thermogenetic activation of Tk-GAL4FruM neurons. Tk-GAL41; fruP1.LexA/ LexAop2-FLPL control males were eliminated from subsequent analyses.
(B-D) Copulation latency (B), duration (C, mean ± S.D.) and unilateral wing extension frequency (D) during thermogenetic activation of Tk-GAL4FruM neurons.
(E) Cumulative copulation curve for males during silencing of Tk-GAL4FruM neurons.
(F-H) Copulation latency (F), duration (G) and unilateral wing extension frequency (H) during silencing of Tk-GAL4FruM neurons.
For (B), (D), (F) and (H), * p < 0.05, n.s.: p > 0.05 (Kruskal-Wallis and post-hoc Mann-Whitney U-tests. For (C) and (G), * p < 0.05, n.s.: p > 0.05 (one-way ANOVA or one-way ANOVA and post-hoc Student's t-tests).
Also see Supplemental Figure S3.