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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Jun;82(11):3897–3900. doi: 10.1073/pnas.82.11.3897

N-Acetylaspartylglutamate: possible role as the neurotransmitter of the lateral olfactory tract.

J M Ffrench-Mullen, K Koller, R Zaczek, J T Coyle, N Hori, D O Carpenter
PMCID: PMC397896  PMID: 2860670

Abstract

N-Acetylaspartylglutamate, an endogenous brain peptide that binds with high affinity to a subpopulation of glutamate-binding sites in rat brain, is excitatory on rat piriform cortex pyramidal cells studied in a perfused brain slice. Both the monosynaptic excitation of the pyramidal cells elicited by stimulation of the lateral olfactory tract and the response to N-acetylaspartylglutamate were blocked by DL-2-amino-4-phosphonobutyrate but not by other excitatory amino acid antagonists. Responses to glutamate and aspartate, previously considered to be candidates as the lateral olfactory tract transmitter, were unaffected by 2-amino-4-phosphonobutyrate. Three days after unilateral bulbectomy there was a significant decrease in concentrations of N-acetylaspartylglutamate as well as aspartate, N-acetylaspartate, and gamma-aminobutyrate in the pyriform cortex of the side from which the olfactory bulb had been removed. These results are consistent with the possibility that N-acetylaspartylglutamate is the endogenous transmitter of the lateral olfactory tract.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avoli M., Barra P. F., Brancati A., Cecchi L., Deodati M. Effetti degli acidi N-acetilaspartico ed N-acetilaspartilglutammico applicati iontoforeticamente in alcune strutture del nevrasse di ratto. Boll Soc Ital Biol Sper. 1976 Sep 30;52(18):1525–1530. [PubMed] [Google Scholar]
  2. Bradford H. F., Richards C. D. Specific release of endogenous glutamate from piriform cortex stimulated in vitro. Brain Res. 1976 Mar 19;105(1):168–172. doi: 10.1016/0006-8993(76)90933-1. [DOI] [PubMed] [Google Scholar]
  3. CURATOLO A., D ARCANGELO P., LINO A., BRANCATI A. DISTRIBUTION OF N-ACETYL-ASPARTIC AND N-ACETYL-ASPARTYL-GLUTAMIC ACIDS IN NERVOUS TISSUE. J Neurochem. 1965 Apr;12:339–342. doi: 10.1111/j.1471-4159.1965.tb06771.x. [DOI] [PubMed] [Google Scholar]
  4. CURTIS D. R., WATKINS J. C. The excitation and depression of spinal neurones by structurally related amino acids. J Neurochem. 1960 Sep;6:117–141. doi: 10.1111/j.1471-4159.1960.tb13458.x. [DOI] [PubMed] [Google Scholar]
  5. Collins G. G. Evidence of a neurotransmitter role for aspartate and gamma-aminobutyric acid in the rat olfactory cortex. J Physiol. 1979 Jun;291:51–60. doi: 10.1113/jphysiol.1979.sp012799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Constanti A., Connor J. D., Galvan M., Nistri A. Intracellularly-recorded effects of glutamate and aspartate on neurones in the guinea-pig olfactory cortex slice. Brain Res. 1980 Aug 18;195(2):403–420. doi: 10.1016/0006-8993(80)90075-x. [DOI] [PubMed] [Google Scholar]
  7. Evans R. H., Francis A. A., Hunt K., Oakes D. J., Watkins J. C. Antagonism of excitatory amino acid-induced responses and of synaptic excitation in the isolated spinal cord of the frog. Br J Pharmacol. 1979 Dec;67(4):591–603. doi: 10.1111/j.1476-5381.1979.tb08706.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans R. H., Francis A. A., Jones A. W., Smith D. A., Watkins J. C. The effects of a series of omega-phosphonic alpha-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br J Pharmacol. 1982 Jan;75(1):65–75. doi: 10.1111/j.1476-5381.1982.tb08758.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godfrey D. A., Ross C. D., Carter J. A., Lowry O. H., Matschinsky F. M. Effect of intervening lesions on amino acid distributions in rat olfactory cortex and olfactory bulb. J Histochem Cytochem. 1980 Nov;28(11):1157–1169. doi: 10.1177/28.11.7430613. [DOI] [PubMed] [Google Scholar]
  10. Harvey J. A., Scholfield C. N., GRAHM L. T., Jr, Aprison M. H. Putative transmitters in denervated olfactory complex. J Neurochem. 1975 Mar;24(3):445–449. doi: 10.1111/j.1471-4159.1975.tb07660.x. [DOI] [PubMed] [Google Scholar]
  11. Hori N., Auker C. R., Braitman D. J., Carpenter D. O. Lateral olfactory tract transmitter: glutamate, aspartate, or neither? Cell Mol Neurobiol. 1981 Mar;1(1):115–120. doi: 10.1007/BF00736043. [DOI] [PubMed] [Google Scholar]
  12. Hori N., Auker C. R., Braitman D. J., Carpenter D. O. Pharmacologic sensitivity of amino acid responses and synaptic activation of in vitro prepyriform neurons. J Neurophysiol. 1982 Dec;48(6):1289–1301. doi: 10.1152/jn.1982.48.6.1289. [DOI] [PubMed] [Google Scholar]
  13. JACOBSON K. B. Studies on the role of N-acetylaspartic acid in mammalian brain. J Gen Physiol. 1959 Nov;43:323–333. doi: 10.1085/jgp.43.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koller K. J., Zaczek R., Coyle J. T. N-acetyl-aspartyl-glutamate: regional levels in rat brain and the effects of brain lesions as determined by a new HPLC method. J Neurochem. 1984 Oct;43(4):1136–1142. doi: 10.1111/j.1471-4159.1984.tb12854.x. [DOI] [PubMed] [Google Scholar]
  15. Miyake M., Kakimoto Y., Sorimachi M. A gas chromatographic method for the determination of N-acetyl-L-aspartic acid, N-acetyl-alpha-aspartylglutamic acid and beta-citryl-L-glutamic acid and their distributions in the brain and other organs of various species of animals. J Neurochem. 1981 Mar;36(3):804–810. doi: 10.1111/j.1471-4159.1981.tb01665.x. [DOI] [PubMed] [Google Scholar]
  16. Monaghan D. T., Holets V. R., Toy D. W., Cotman C. W. Anatomical distributions of four pharmacologically distinct 3H-L-glutamate binding sites. Nature. 1983 Nov 10;306(5939):176–179. doi: 10.1038/306176a0. [DOI] [PubMed] [Google Scholar]
  17. Nadler J. V., Cooper J. R. N-acetyl-L-aspartic acid content of human neural tumours and bovine peripheral nervous tissues. J Neurochem. 1972 Feb;19(2):313–319. doi: 10.1111/j.1471-4159.1972.tb01341.x. [DOI] [PubMed] [Google Scholar]
  18. Reichelt K. L., Fonnum F. Subcellular localization of N-acetyl-aspartyl-glutamate, N-acetyl-glutamate and glutathione in brain. J Neurochem. 1969 Sep;16(9):1409–1416. doi: 10.1111/j.1471-4159.1969.tb05993.x. [DOI] [PubMed] [Google Scholar]
  19. Scholfield C. N., Moroni F., Corradetti R., Pepeu G. Levels and synthesis of glutamate and aspartate in the olfactory cortex following bulbectomy. J Neurochem. 1983 Jul;41(1):135–138. doi: 10.1111/j.1471-4159.1983.tb11824.x. [DOI] [PubMed] [Google Scholar]
  20. TALLAN H. H., MOORE S., STEIN W. H. N-Acetyl-L-aspartic acid in brain. J Biol Chem. 1956 Mar;219(1):257–264. [PubMed] [Google Scholar]
  21. Watkins J. C., Evans R. H. Excitatory amino acid transmitters. Annu Rev Pharmacol Toxicol. 1981;21:165–204. doi: 10.1146/annurev.pa.21.040181.001121. [DOI] [PubMed] [Google Scholar]
  22. Whittemore S. R., Mena E. E., Monaghan D. T., Cotman C. W. Regional distribution and ionic requirement of Cl-/Ca2+-activated and Cl-/Ca2+-independent glutamate receptors in rat brain. Brain Res. 1983 Oct 24;277(1):99–107. doi: 10.1016/0006-8993(83)90911-3. [DOI] [PubMed] [Google Scholar]
  23. Yamamoto C., Matsui S. Effect of stimulation of excitatory nerve tract on release of glutamic acid from olfactory cortex slices in vitro. J Neurochem. 1976 Mar;26(3):487–491. doi: 10.1111/j.1471-4159.1976.tb01500.x. [DOI] [PubMed] [Google Scholar]
  24. Zaczek R., Koller K., Cotter R., Heller D., Coyle J. T. N-acetylaspartylglutamate: an endogenous peptide with high affinity for a brain "glutamate" receptor. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1116–1119. doi: 10.1073/pnas.80.4.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]

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