Abstract
Soybean seeds [Glycine max (L.) Merr. ev. Bragg] were mutagenized with ethyl methanesulfonate. The M2 progeny (i.e., the first generation after mutagenesis) of these seeds were screened for increased nodulation under high nitrate culture conditions. Fifteen independent nitrate-tolerant symbiotic (nts) mutants were obtained from 2500 M2 families. In culture on sand with KNO3, nodule mass and nodule number in mutant lines were several-fold those of the wild type cultured under the same conditions. Inheritance of the nts character through to subsequent generations was observed in the 10 mutants tested. Mutant nts382 also nodulated more than the wild type in the absence of nitrate. Furthermore, nitrate stimulated growth in both the wild type and nts382, and these lines had similar nitrate reductase activity. These results indicate that nts382 is affected in a nodule-development regulatory gene and not in a gene related to nitrate assimilation.
Keywords: nitrate inhibition, nts mutants, nitrate-tolerant symbiosis, ethyl methanesulfonate mutagenesis, nitrogen fixation
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- Hardy R. W., Holsten R. D., Jackson E. K., Burns R. C. The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation. Plant Physiol. 1968 Aug;43(8):1185–1207. doi: 10.1104/pp.43.8.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herridge D. F. Use of the ureide technique to describe the nitrogen economy of field-grown soybeans. Plant Physiol. 1982 Jul;70(1):7–11. doi: 10.1104/pp.70.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kosslak R. M., Bohlool B. B. Suppression of nodule development of one side of a split-root system of soybeans caused by prior inoculation of the other side. Plant Physiol. 1984 May;75(1):125–130. doi: 10.1104/pp.75.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McNeil D. L. Variations in Ability of Rhizobium japonicum Strains To Nodulate Soybeans and Maintain Fixation in the Presence of Nitrate. Appl Environ Microbiol. 1982 Sep;44(3):647–652. doi: 10.1128/aem.44.3.647-652.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicholas J. C., Harper J. E., Hageman R. H. Nitrate Reductase Activity in Soybeans (Glycine max [L.] Merr.): I. Effects of Light and Temperature. Plant Physiol. 1976 Dec;58(6):731–735. doi: 10.1104/pp.58.6.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce M., Bauer W. D. A rapid regulatory response governing nodulation in soybean. Plant Physiol. 1983 Oct;73(2):286–290. doi: 10.1104/pp.73.2.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigaud J., Puppo A. Effect of nitrite upon leghemoglobin and interaction with nitrogen fixation. Biochim Biophys Acta. 1977 May 26;497(3):702–706. doi: 10.1016/0304-4165(77)90291-4. [DOI] [PubMed] [Google Scholar]
- Streeter J. G. Effect of nitrate in the rooting medium on carbohydrate composition of soybean nodules. Plant Physiol. 1981 Oct;68(4):840–844. doi: 10.1104/pp.68.4.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Streeter J. G. Synthesis and accumulation of nitrite in soybean nodules supplied with nitrate. Plant Physiol. 1982 Jun;69(6):1429–1434. doi: 10.1104/pp.69.6.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]