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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Jun;82(12):4291–4295. doi: 10.1073/pnas.82.12.4291

Sequence and expression of the rat prodynorphin gene.

O Civelli, J Douglass, A Goldstein, E Herbert
PMCID: PMC397984  PMID: 3858883

Abstract

We report here the isolation of a lambda genomic clone that contains the nucleotide sequence coding for the main exon of the rat prodynorphin (proenkephalin B) gene. This exon codes for the majority of the translated region of prodynorphin mRNA including the opioid peptides alpha-neo-endorphin, dynorphin A, and dynorphin B. The entire 3' untranslated region is also contained on the lambda clone. Nucleotide sequence comparison with the main exon of the human prodynorphin gene reveals both structural and sequence homology. RNA blot analysis reveals that prodynorphin transcripts can be seen in numerous regions of the rat brain and in the adrenal gland, spinal cord, testis, and anterior pituitary.

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Selected References

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  1. Civelli O., Birnberg N., Herbert E. Detection and quantitation of pro-opiomelanocortin mRNA in pituitary and brain tissues from different species. J Biol Chem. 1982 Jun 25;257(12):6783–6787. [PubMed] [Google Scholar]
  2. Cone R. I., Weber E., Barchas J. D., Goldstein A. Regional distribution of dynorphin and neo-endorphin peptides in rat brain, spinal cord, and pituitary. J Neurosci. 1983 Nov;3(11):2146–2152. doi: 10.1523/JNEUROSCI.03-11-02146.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Day R., Lemaire I., Mercier P., Beaudoin H., Lemaire S. Asbestos-related increase in pulmonary levels of vasoactive intestinal peptide (VIP). Life Sci. 1983 Nov 7;33(19):1869–1876. doi: 10.1016/0024-3205(83)90671-9. [DOI] [PubMed] [Google Scholar]
  4. Fischli W., Goldstein A., Hunkapiller M. W., Hood L. E. Isolation and amino acid sequence analysis of a 4,000-dalton dynorphin from porcine pituitary. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5435–5437. doi: 10.1073/pnas.79.17.5435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedman H. J., Jen M. F., Chang J. K., Lee N. M., Loh H. H. Dynorphin: a possible modulatory peptide on morphine or beta-endorphin analgesia in mouse. Eur J Pharmacol. 1981 Jan 29;69(3):357–360. doi: 10.1016/0014-2999(81)90483-0. [DOI] [PubMed] [Google Scholar]
  6. Goldstein A., Fischli W., Lowney L. I., Hunkapiller M., Hood L. Porcine pituitary dynorphin: complete amino acid sequence of the biologically active heptadecapeptide. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7219–7223. doi: 10.1073/pnas.78.11.7219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldstein A., Ghazarossian V. E. Immunoreactive dynorphin in pituitary and brain. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6207–6210. doi: 10.1073/pnas.77.10.6207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldstein A., Tachibana S., Lowney L. I., Hunkapiller M., Hood L. Dynorphin-(1-13), an extraordinarily potent opioid peptide. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6666–6670. doi: 10.1073/pnas.76.12.6666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herman B. H., Leslie F., Goldstein A. Behavioral effects and in vivo degradation of intraventricularly administered dynorphin-(1-13) and D-Ala2-dynorphin-(1-11) in rats. Life Sci. 1980 Sep 8;27(10):883–892. doi: 10.1016/0024-3205(80)90084-3. [DOI] [PubMed] [Google Scholar]
  10. Horikawa S., Takai T., Toyosato M., Takahashi H., Noda M., Kakidani H., Kubo T., Hirose T., Inayama S., Hayashida H. Isolation and structural organization of the human preproenkephalin B gene. Nature. 1983 Dec 8;306(5943):611–614. doi: 10.1038/306611a0. [DOI] [PubMed] [Google Scholar]
  11. Hughes J., Smith T. W., Kosterlitz H. W., Fothergill L. A., Morgan B. A., Morris H. R. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577–580. doi: 10.1038/258577a0. [DOI] [PubMed] [Google Scholar]
  12. Jingami H., Nakanishi S., Imura H., Numa S. Tissue distribution of messenger RNAs coding for opioid peptide precursors and related RNA. Eur J Biochem. 1984 Aug 1;142(3):441–447. doi: 10.1111/j.1432-1033.1984.tb08306.x. [DOI] [PubMed] [Google Scholar]
  13. Kakidani H., Furutani Y., Takahashi H., Noda M., Morimoto Y., Hirose T., Asai M., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor. Nature. 1982 Jul 15;298(5871):245–249. doi: 10.1038/298245a0. [DOI] [PubMed] [Google Scholar]
  14. Kilpatrick D. L., Wahlstrom A., Lahm H. W., Blacher R., Udenfriend S. Rimorphin, a unique, naturally occurring [Leu]enkephalin-containing peptide found in association with dynorphin and alpha-neo-endorphin. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6480–6483. doi: 10.1073/pnas.79.21.6480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kromer W., Höllt V., Schmidt H., Herz A. Release of immunoreactive-dynorphin from the isolated guinea-pig small intestine is reduced during peristaltic activity. Neurosci Lett. 1981 Aug 7;25(1):53–56. doi: 10.1016/0304-3940(81)90100-2. [DOI] [PubMed] [Google Scholar]
  16. Minamino N., Kangawa K., Chino N., Sakakibara S., Matsuo H. Beta-neo-endorphin, a new hypothalamic "big" Leu-enkephalin of porcine origin: its purification and the complete amino acid sequence. Biochem Biophys Res Commun. 1981 Apr 15;99(3):864–870. doi: 10.1016/0006-291x(81)91243-2. [DOI] [PubMed] [Google Scholar]
  17. Morley J. E., Levine A. S. Dynorphin-(1-13) induces spontaneous feeding in rats. Life Sci. 1981 Nov 2;29(18):1901–1903. doi: 10.1016/0024-3205(81)90522-1. [DOI] [PubMed] [Google Scholar]
  18. Palkovits M., Brownstein M. J., Zamir N. On the origin of dynorphin A and alpha-neo-endorphin in the substantia nigra. Neuropeptides. 1984 May;4(3):193–199. doi: 10.1016/0143-4179(84)90100-8. [DOI] [PubMed] [Google Scholar]
  19. Przewłocki R., Lasón W., Konecka A. M., Gramsch C., Herz A., Reid L. D. The opioid peptide dynorphin, circadian rhythms, and starvation. Science. 1983 Jan 7;219(4580):71–73. doi: 10.1126/science.6129699. [DOI] [PubMed] [Google Scholar]
  20. Seizinger B. R., Grimm C., Höllt V., Herz A. Evidence for a selective processing of proenkephalin B into different opioid peptide forms in particular regions of rat brain and pituitary. J Neurochem. 1984 Feb;42(2):447–457. doi: 10.1111/j.1471-4159.1984.tb02698.x. [DOI] [PubMed] [Google Scholar]
  21. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  22. Spampinato S., Goldstein A. Immunoreactive dynorphin in rat tissues and plasma. Neuropeptides. 1983 Jan;3(3):193–212. doi: 10.1016/0143-4179(83)90016-1. [DOI] [PubMed] [Google Scholar]
  23. Suda M., Nakao K., Yoshimasa T., Ikeda Y., Sakamoto M., Yanaihara N., Numa S., Imura H. A novel opioid peptide, leumorphin, acts as an agonist at the kappa opiate receptor. Life Sci. 1983 Jun 13;32(24):2769–2775. doi: 10.1016/0024-3205(83)90398-3. [DOI] [PubMed] [Google Scholar]
  24. Tachibana S., Araki K., Ohya S., Yoshida S. Isolation and structure of dynorphin, an opioid peptide, from porcine duodenum. Nature. 1982 Jan 28;295(5847):339–340. doi: 10.1038/295339a0. [DOI] [PubMed] [Google Scholar]
  25. Tang F., Costa E., Schwartz J. P. Increase of proenkephalin mRNA and enkephalin content of rat striatum after daily injection of haloperidol for 2 to 3 weeks. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3841–3844. doi: 10.1073/pnas.80.12.3841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Watson S. J., Akil H., Fischli W., Goldstein A., Zimmerman E., Nilaver G., van wimersma Griedanus T. B. Dynorphin and vasopressin: common localization in magnocellular neurons. Science. 1982 Apr 2;216(4541):85–87. doi: 10.1126/science.6121376. [DOI] [PubMed] [Google Scholar]
  28. Watson S. J., Akil H., Ghazarossian V. E., Goldstein A. Dynorphin immunocytochemical localization in brain and peripheral nervous system: preliminary studies. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1260–1263. doi: 10.1073/pnas.78.2.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weber E., Roth K. A., Barchas J. D. Colocalization of alpha-neo-endorphin and dynorphin immunoreactivity in hypothalamic neurons. Biochem Biophys Res Commun. 1981 Dec 15;103(3):951–958. doi: 10.1016/0006-291x(81)90902-5. [DOI] [PubMed] [Google Scholar]
  30. Yamamoto Y., Yanaihara C., Katsumaru Y., Mochizuki T., Tobe A., Egawa M., Imura H., Numa S., Yanaihara N. Synthesis of porcine leumorphin and some of its biological activities. Regul Pept. 1983 May;6(2):163–168. doi: 10.1016/0167-0115(83)90009-5. [DOI] [PubMed] [Google Scholar]

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