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. 1995 Feb 15;14(4):650–659. doi: 10.1002/j.1460-2075.1995.tb07043.x

Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74.

V Brault 1, J F van den Heuvel 1, M Verbeek 1, V Ziegler-Graff 1, A Reutenauer 1, E Herrbach 1, J C Garaud 1, H Guilley 1, K Richards 1, G Jonard 1
PMCID: PMC398128  PMID: 7882968

Abstract

Beet western yellows luteovirus is obligately transmitted by the aphid Myzus persicae in a circulative, non-propagative fashion. Virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. Virions contain two types of protein; the major 22 kDa capsid protein and the minor read-through protein, P74, which is composed of the major capsid protein fused by translational read-through to a long C-terminal extension called the read-through domain. Beet western yellows virus carrying various mutations in the read-through domain was tested for its ability to be transmitted to test plants by aphids fed on agro-infected plants and semi-purified or purified virus preparations. The results establish that the read-through domain carries determinants that are essential for aphid transmission. The findings also reveal that the read-through domain is important for accumulation of the virus in agro-infected plants.

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Selected References

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