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. 1995 Feb 15;14(4):660–666. doi: 10.1002/j.1460-2075.1995.tb07044.x

Inhibition of flower formation by antisense repression of mitochondrial citrate synthase in transgenic potato plants leads to a specific disintegration of the ovary tissues of flowers.

V Landschütze 1, L Willmitzer 1, B Müller-Röber 1
PMCID: PMC398129  PMID: 7882969

Abstract

The tricarboxylic acid (TCA) cycle constitutes a major component of the mitochondrial metabolism of eucaryotes, including higher plants. To analyze the importance of this pathway, we down-regulated mitochondrial citrate synthase (mCS; EC 4.1.3.7), the first enzyme of the TCA cycle, in transgenic potato plants using an antisense RNA approach. Several transformants were identified with reduced citrate synthase activity (down to approximately 6% of wild-type activity). These plants were indistinguishable from wild-type plants in the greenhouse during vegetative growth. A major change, however, was seen upon initiation of the generative phase (flower formation). In the case of transgenic plants with a strong reduction in citrate synthase activity (< 30% of wild-type levels), flower buds formed > 2 weeks later as compared with wild-type plants. Furthermore, flower buds from these plants did not develop into mature flowers but rather were aborted at an early stage of development. Microscopic analysis showed that in these cases ovaries disintegrated during flower development. We conclude that the TCA cycle is of major importance during the transition from the vegetative to the generative phase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernier G., Havelange A., Houssa C., Petitjean A., Lejeune P. Physiological Signals That Induce Flowering. Plant Cell. 1993 Oct;5(10):1147–1155. doi: 10.1105/tpc.5.10.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. doi: 10.1093/nar/12.22.8711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bodson M., Outlaw W. H. Elevation in the Sucrose Content of the Shoot Apical Meristem of Sinapis alba at Floral Evocation. Plant Physiol. 1985 Oct;79(2):420–424. doi: 10.1104/pp.79.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Couée I., Defontaine S., Carde J. P., Pradet A. Effects of anoxia on mitochondrial biogenesis in rice shoots: modification of in organello translation characteristics. Plant Physiol. 1992 Feb;98(2):411–421. doi: 10.1104/pp.98.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Deblaere R., Bytebier B., De Greve H., Deboeck F., Schell J., Van Montagu M., Leemans J. Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Res. 1985 Jul 11;13(13):4777–4788. doi: 10.1093/nar/13.13.4777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garfin D. E. One-dimensional gel electrophoresis. Methods Enzymol. 1990;182:425–441. doi: 10.1016/0076-6879(90)82035-z. [DOI] [PubMed] [Google Scholar]
  8. Hanning I., Heldt H. W. On the Function of Mitochondrial Metabolism during Photosynthesis in Spinach (Spinacia oleracea L.) Leaves (Partitioning between Respiration and Export of Redox Equivalents and Precursors for Nitrate Assimilation Products). Plant Physiol. 1993 Dec;103(4):1147–1154. doi: 10.1104/pp.103.4.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanson M. R. Plant mitochondrial mutations and male sterility. Annu Rev Genet. 1991;25:461–486. doi: 10.1146/annurev.ge.25.120191.002333. [DOI] [PubMed] [Google Scholar]
  10. Hernould M., Suharsono S., Litvak S., Araya A., Mouras A. Male-sterility induction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat. Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2370–2374. doi: 10.1073/pnas.90.6.2370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kagawa T. Organelle-specific isozymes of citrate synthase in the endosperm of developing ricinus seedlings. Plant Physiol. 1981 Oct;68(4):845–850. doi: 10.1104/pp.68.4.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Krömer S., Heldt H. W. On the Role of Mitochondrial Oxidative Phosphorylation in Photosynthesis Metabolism as Studied by the Effect of Oligomycin on Photosynthesis in Protoplasts and Leaves of Barley (Hordeum vulgare). Plant Physiol. 1991 Apr;95(4):1270–1276. doi: 10.1104/pp.95.4.1270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laughnan J. R., Gabay-Laughnan S. Cytoplasmic male sterility in maize. Annu Rev Genet. 1983;17:27–48. doi: 10.1146/annurev.ge.17.120183.000331. [DOI] [PubMed] [Google Scholar]
  14. Levings C. S., 3rd Thoughts on Cytoplasmic Male Sterility in cms-T Maize. Plant Cell. 1993 Oct;5(10):1285–1290. doi: 10.1105/tpc.5.10.1285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Monéger F., Mandaron P., Niogret M. F., Freyssinet G., Mache R. Expression of Chloroplast and Mitochondrial Genes during Microsporogenesis in Maize. Plant Physiol. 1992 Jun;99(2):396–400. doi: 10.1104/pp.99.2.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rocha-Sosa M., Sonnewald U., Frommer W., Stratmann M., Schell J., Willmitzer L. Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J. 1989 Jan;8(1):23–29. doi: 10.1002/j.1460-2075.1989.tb03344.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vervliet G., Holsters M., Teuchy H., Van Montagu M., Schell J. Characterization of different plaque-forming and defective temperate phages in Agrobacterium. J Gen Virol. 1975 Jan;26(1):33–48. doi: 10.1099/0022-1317-26-1-33. [DOI] [PubMed] [Google Scholar]
  18. des Francs-Small C. C., Ambard-Bretteville F., Darpas A., Sallantin M., Huet J. C., Pernollet J. C., Rémy R. Variation of the polypeptide composition of mitochondria isolated from different potato tissues. Plant Physiol. 1992 Jan;98(1):273–278. doi: 10.1104/pp.98.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]

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