Table 1. FcγR binding of analyzed antibodies.
FcγRI § | FcγRIIa H131 | FcγRIIa R131 | FcγRIIb | FcγRIIIa V158 | FcγRIIIa F158 | FcγRIIIb | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KA | Fold | KA | Fold | KA | Fold | KA | Fold | KA | Fold | KA | Fold | KA | Fold | |
IgG1 wt | 8,333.33 | 1.0 | 1.22 | 1.0 | 1.25 | 1.0 | 0.42 | 1.0 | 4.00 | 1.0 | 0.77 | 1.0 | 0.09 | 1.0 |
S239D/I332E | 50,000.00 | 6.0 | 5.00 | 4.1 | 10.99 | 8.8 | 7.14 | 17 | 76.92 | 18 | 24.39 | 32 | 1.76 | 18.7 |
S239D/I332E/G236A | 22,727.27 | 2.7 | 27.78 | 23 | 66.67 | 54 | 6.67 | 16 | 45.45 | 11 | 21.74 | 29 | 1.42 | 15.0 |
wt-fucose -/ -§ | 8,333.33 | 1.0 | 0.91 | 0.75 | 2.50 | 2.0 | 0.42 | 1.0 | 55.56 | 13.9 | 10.20 | 13.3 | n.d. | - |
KA’s were obtained from global Langmuir fits of Biacore data and are presented in µM−1. Fold = KA(IgG1 wt) / (KA(variant). § KA’s for FcγRI as well as data for wt-fucose −/− were taken from ref. 47.