Video 1. Example of unidirectional motion of a Cy3-h^WT RNP towards the minus end of a polarity-marked microtubule.

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DOI: 10.7554/eLife.01596.005

RNA, pseudocoloured magenta; microtubule, pseudocoloured green. Microtubule plus end is labelled with a highly fluorescent segment. This RNP pauses upon reaching the minus end of the microtubule; we also observed instances of dissociation of unidirectional RNPs upon reaching the minus end (Figure 6D,E). Video corresponds to 12.4 s; width of the image is 16.7 μm.

DOI: http://dx.doi.org/10.7554/eLife.01596.005