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. 2014 Feb 14;53(9):1521–1526. doi: 10.1021/bi401556p

Figure 3.

Figure 3

DNA methylation by M.SssI using different AdoMet analogues produced by the AdoMet synthetase-catalyzed activation of methionine derivatives. The methionine substrates included l-methionine (Met), l-methionine methyl (MME) and ethyl (MEE) esters produced by C. jejuni AdoMet synthetase and tested at 100 ± 5 μM, and N-acetyl-l-methionine (NAM) and N,N-dimethylmethionine (DMM) produced by N. meningitidis AdoMet synthetase and tested at ∼10 and <5 μM, respectively. The methylation of 1 μg of linearized pUC19 DNA was conducted for 1 h at 37 °C, followed by digestion with HpaII and gel electrophoresis.