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Methylation of epinephrine by COMTase using different AdoMet analogues. (A) HPLC separation of the catechol O-methyltransferase reaction mixture to allow quantitation of the S-adenosylhomocysteine (AdoHcy) product. (B) Time course for the production of S-adenosylhomocysteine during the methylation of epinephrine using AdoMet (◆), AdoMet methyl ester (■), or AdoMet ethyl ester (▲) as the methyl donor.
