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ABA was added to yeast cells suspended in 20 mM MES buffer (pH4.7) and ABACUS1 fluorescence was measured after 5 min. DxAm/DxDm values for yeast samples plus ABA were normalized to corresponding mock treated samples. EV = empty vector control. Because ABACUS1 is stereospecific for (+)-ABA the effective ABA concentration available for sensing is ½ the (±)-ABA concentration.
