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. 2014 Apr 3;11:28. doi: 10.1186/1742-4690-11-28

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Copyright © 2014 Gregory et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Co-capture using cellular glycoproteins. Co-capture was performed on virus produced from cells transfected as before using VSV-G and the indicated amounts of GFP-tagged cellular proteins or YFP-tagged MLV Env. A) The surface expression of the indicated proteins in transfected cells was assayed by surface labeling GFP with an anti-GFP antibody conjugated to Alexa Fluor 647, which was detected by flow cytometry. B) The luciferase signal from the anti-GFP captured samples and the no-antibody captured samples normalized to the straight control is shown. The average fold increase of antibody/no antibody is shown for each pair. All graphs are the average of at least three independent experiments and standard deviations are shown. *p < 0.05; **p < 0.01; NS p > 0.05.