Table 1. Data Collection and Scattering-Derived Parameters.
| unmodified Skp1 | GGFGGn-Skp1 | |
|---|---|---|
| Data Collection Parameters | ||
| beamline | SSRL beamline 4-2 | |
| beam geometry | 0.3 mm × 0.3 mm, pinhole camera | |
| detector | Rayonix MX255-HE | |
| wavelength (Å) | 1.1263 | |
| q range (Å–1) | 0.03–0.30 | |
| exposure time (s) | 12 × 1 | |
| temperature (K) | 283 | |
| concentration range (mg/mL) | 1.2–9.4 | 1.5–3.0 |
| Structural Parametersa | ||
| I(0) (real space) | 7438 ± 3 | 3191 ± 6 |
| Rg (Å) (real space) | 25.3 ± 0.1 | 27.2 ± 0.1 |
| I(0) (Guinier) | 7130 ± 10 | 3158 ± 7 |
| Rg (Å) (Guinier) | 23.9 ± 1.1 | 26.6 ± 0.6 |
| Dmax (Å) | 79 | 91 |
| Porod volume estimate (Å3) | 64693 | 76000 |
| DAMMIF volume (Å3)b | 87753 | 98971 |
| mean NSDc of 10 DAMMIF models | 0.750 ± 0.080 | 0.728 ± 0.059 |
| Molecular Mass Determinationa | ||
| calculated monomeric Mr from sequence | 18587 | 19481 |
| molecular mass Mr estimate | ||
| from SAXS MoW | 38800 | 46400 |
| from SCÅTTER (real space) | 38800 | 40200 |
| from SCÅTTER (Guinier) | 40300 | 43900 |
| Software | ||
| primary data reduction | SAXSPipe (SSRL) | |
| data processing | PRIMUS, GNOM, SCÅTTER, SAXS MoW | |
| ab initio analysis | DAMMIF | |
| validation and averaging | DAMAVER | |
| three-dimensional graphics representations | PyMOL | |
a
Reported for averaged data over a concentration range.
b
Reported for the highest-concentration data.
c
Normalized spatial discrepancy.