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Peptides unique to other AHA isoforms enrich with Ntapi:AHA1 after TAP-tag-mediated purification. Metabolically labeled 14NNtapi:gAHA1/15Nwild-type (red) and reciprocal 14Nwild-type/15NNtapi:gAHA1 (blue) plasma membrane pairs were incubated with IgG resin, washed, and eluted overnight with TEV protease. The raw data was inspected manually for peaks corresponding to the indicated unique peptides from AHA1, AHA9, and AHA11.
