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High-frequency RR spectra of RFQ samples of the reaction of 1 equiv of hemin with (A) Y75A apo-HasAp and (B) H83A apo-HasAp (protein concentration after mixing of 300 μM and excitation wavelength of 406 nm; all spectra are normalized to the intensity of the ν4 feature observed between 1371 and 1377 cm–1).
