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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Biochemistry. 2008 Mar 21;47(15):4345–4358. doi: 10.1021/bi701778x

Figure 5.

Figure 5

Representative ITC thermograms (upper) and isotherms (lower) for Pot1pN titrated with noncognate oligonucleotides in 50 mM Na2HPO4 at pH 7.5, 150 mM NaCl, and 1 mM BME at 20 °C. d(IGTTAC), 25 injections of 99 μM G1I titrated into 6 μM Pot1pN; d(GITTAC), 60 injections of 500 μM G2I titrated into 43 μM Pot1pN; d(GGUTAC), 25 injections of 144 μM T3dU titrated into 6 μM Pot1pN; and d(GGTUAC), 70 injections of 880 μM T4dU titrated into 43 μM Pot1pN.