Table 1.
Thermodynamic Parameters for Pot1pN Binding to d(GGTTAC) by ITC at Indicated Temperatures in 50 mM Na2HPO4 at pH 7.5, 150 mM NaCl, and 1 mM BME
| temperature (°C) | n | Kd (nM) | ΔG (kcal/mol) | ΔHcal (kcal/mol) | –TΔS (kcal/mol) |
|---|---|---|---|---|---|
| 5 | 1.02 ± 0.06 | 4.1 ± 1.6 | –10.7 ± 0.2 | –16.3 ± 0.4 | 5.6 ± 0.6 |
| 10 | 0.94 ± 0.02 | 3.6 ± 1.0 | –11.0 ± 0.2 | –18.9 ± 0.5 | 7.9 ± 0.6 |
| 15 | 0.97 ± 0.09 | 6.0 ± 1.4 | –10.9 ± 0.1 | –20.8 ± 0.6 | 9.9 ± 0.8 |
| 20 | 1.02 ± 0.03 | 9.7 ± 1.1 | –10.8 ± 0.1 | –22.8 ± 0.8 | 12.0 ± 0.8 |
| 25 | 1.03 ± 0.07 | 15.0 ± 3.1 | –10.7 ± 0.1 | –24.3 ± 0.6 | 13.6 ± 0.7 |
| 30 | 1.02 ± 0.05 | 27.3 ± 4.5 | –10.5 ± 0.1 | –25.3 ± 0.8 | 14.8 ± 0.9 |
| 35 | 1.06 ± 0.02 | 57.0 ± 4.1 | –10.2 ± 0.04 | –28.2 ± 0.1 | 18.0 ± 0.04 |
| ΔCpcal (cal mol–1 K–1) | –372 ± 19 | ||||
| TH (K) | 233 ± 3 | ||||
| TS (K) | 338 |