Table 2.

Ex vivo phenotyping of Tetramer⁺ CD4 T cells of DRB1*0101 healthy adults with RV peptide specific TCLs.

Healthy Adult	Peptide Tetramer	#Tet⁺/6.0×10⁵ CD4⁺ T cells	% Antigen experienced T cells^*	% α4β7(+) CCR9(−)^#	% α4β7(+) CCR9(+)^#	% α4β7(−) CCR9(−)^#	% α4β7(−) CCR9(+)^#
HA-02	TRF	8	54.5	33.3	33.3	33.3	0
	FLU	36	68.4	42.3	7.69	50	0
	NSP2-3	10	53.8	85.7	14.3	0	0
	VP3-4	16	61.1	54.5	9.09	27.3	9.09
	VP6-7	28	85.7	63.3	26.7	3.33	6.67
HA-15	TRF	4	0	^¥	^¥	^¥	^¥
	FLU	30	83.3	25	12.5	62.5	0
	VP3-4	16	23.1	50	0	50	0
	VP6-7	22	63.2	63.6	27.3	9.09	0
HA-19	TRF	10	85.7	8.33	33.3	33.3	25
	FLU	24	65.7	21.7	0	78.3	0
	NSP2-3	18	48.1	30.8	7.69	61.5	0
	VP6-7	26	64.1	28	4	64	4
HA-23	TRF	8	57.1	25	25	50	0
	FLU	60	91.2	32.7	3.85	61.5	1.92
	VP6-7	24	87	75	15	10	0
HA-29	TRF	14	50	0	50	50	0
	FLU	48	59.3	6.25	12.5	81.2	0
	VP6-7	32	38.9	57.1	14.3	28.6	0
HA-41	TRF	3	100	33.3	0	66.7	0
	FLU	42	97.1	3.03	0	90.9	6.06
	VP6-7	25	67.3	50	0	50	0
HA-51	TRF	98	36.7	44.8	20.7	27.6	6.9
	FLU	155	40.6	50.7	15.5	31	2.82
	NSP2-3	63	31.7	34.6	30.8	26.9	7.69
	VP3-4	54	37.5	45.8	12.5	29.2	8.33
	VP6-7	50	41.5	58.8	23.5	5.88	11.8
HA-52	TRF	72	27.3	66.7	0	33.3	0
	FLU	284	25.4	40.3	8.06	50	1.61
	NSP2-3	140	17	55.6	11.1	27.8	5.56
	VP3-4	149	22.6	35.7	0	57.1	7.14
	VP6-7	134	16.5	31.2	25	43.7	0

Antigen experienced cells are CD62L⁻,CD45RA^+/− and CD62L⁺ CD45RA⁻ (Figure 4B).

Percentages correspond to antigen experienced cells.

^¥

, No antigen experienced cells were identified.