Abstract
Bacillus pumilus is a Gram-positive, rod-shaped, aerobic bacterium isolated from the soil. B. pumilus strain B6033 was originally selected as a biocatalyst for the stereospecific oxidation of β-lactams. Here, we present a 3.8-Mb assembly of its genome, which is the second fully assembled genome of a B. pumilus strain.
GENOME ANNOUNCEMENT
Bacillus pumilus is a soil bacterium with cellular features similar to those of other members of the Bacillus genus. Various attributes of the species have been exploited industrially, and B. pumilus strain B6033, originally isolated in India, was selected in a screen for a biocatalyst to effect the stereospecific oxidation of β-lactams to their (R)-sulfoxide derivatives (1). Subsequently, the enzyme responsible for the oxidation was isolated, and based on its dual capability to react as a catalase (H2O2 disproportionation) and a peroxidase (oxidation of a typical substrate), it was concluded to be a catalase-peroxidase (KatG) but with physical properties somewhat different from those of all other characterized KatGs (1). The putative existence of such an unusual KatG was of interest because it had the potential to shed light on the evolution and in vivo role of an extensively studied class of relatively new enzymes, but one which still presented many questions (2). KatGs are best known for their role in the activation of isoniazid as an antituberculosis drug, wherein mutations in the katG gene give rise to isoniazid resistance in Mycobacterium tuberculosis (3). KatGs are phylogenetically and structurally linked to the peroxidase family (4, 5), but their catalatic activity is predominant by several orders of magnitude. A side-by-side comparison has revealed a family with remarkably similar properties, making the enzyme from B. pumilus an apparent outlier and therefore interesting in its own right (6). In order to produce the large quantities of protein needed for a complete characterization, we wanted to clone the putative katG gene, and therefore, we set out to determine the sequence of the genome.
The genome of B. pumilus B6033 was sequenced in two stages. The first stage employed data generated using an Illumina MiSeq platform, which was assembled into 14 contigs using a combination of MIRA Assembler version 3.9.3 (7), Velvet version 1.2.08 (8), the MUMmer version 3.23 (9) package, and some Sanger sequencing. The second stage to complete the genome utilized a Pacific Biosciences data set generated by Genome Québec, which was assembled using the PacBio SMRT Analysis pipeline version 2.0.1, with 172× coverage to give a single contiguous genome sequence. The 14 contigs from the Illumina data were aligned for confirmation. The sequence was annotated by the National Center for Biotechnology Information (NCBI) Prokaryotic Genome Annotation Pipeline.
The genome sequence of B. pumilus MTCC B6033 consists of 3,763,493 bases, with a G+C content of 41.4%. There are 3,659 putative coding sequences, 81 tRNA genes, and 6 rRNA clusters. A comparison of the genome with the only other completed B. pumilus genome, that of strain SAFR-032 (accession no. NC_009848.1) (10), using Mauve 2.3.1 (11) revealed 92% identity, with rearrangements of only two small sections. Relevant to the initial purpose of the work, a catalase-peroxidase gene was not found. However, two functional catalase genes were found, one for a typical clade 1 monofunctional heme catalase and the second for a manganese catalase, in addition to a cryptic gene for a monofunctional catalase.
Nucleotide sequence accession number.
The genome sequence of B. pumilus MTCC B6033 was deposited with NCBI GenBank under the accession no. CP007436.1.
ACKNOWLEDGMENTS
This work was supported by a grant (DG9600) from the Natural Sciences and Engineering Research Council (NSERC) of Canada and by the Canadian Research Chairs Program (CRC), both to P.C.L.
We acknowledge the support of Genome Québec and Genome Canada for funding to the Innovation Centre where the PacBio sequencing was performed. We also acknowledge support for the Manitoba next-generation sequencing platform provided by the Manitoba Institute of Child Health, the CancerCare Manitoba Foundation, the Canadian Foundation for Innovation, Province of Manitoba, University of Manitoba Faculty of Medicine, Manitoba Health Research Council, and Manitoba Institute of Cell Biology. We thank R. S. Jolly and the Microbial Type Culture Collection (IMTECH, Chandigarh, India) for providing us with the B. pumilus MTCC B6033 strain.
Footnotes
Citation Villanueva J, Switala J, Ivancich A, Loewen PC. 2014. Genome sequence of Bacillus pumilus MTCC B6033. Genome Announc. 2(2):e00327-14. doi:10.1128/genomeA.00327-14.
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