The Effect of Preceding Biopsy on Complete Endoscopic Resection in Rectal Carcinoid Tumor

Abstract

Biopsy of rectal carcinoid tumor is commonly taken before endoscopic resection. However the preceding biopsy can inhibit complete resection by causing blurred tumor border and fibrosis of the tissue. The objective of the study was to investigate the effect of preceding biopsy on complete endoscopic resection in rectal carcinoid tumor. It was also determined if rectal carcinoid tumors can be macroscopically distinguished by endoscopy. We reviewed retrospectively the records of patients with rectal carcinoid tumor who had undergone an endoscopic treatment at our hospital, during a 7-yr period. The resection margin was clear in 57 of 98 cases. The preceding biopsy was taken in 57 cases and the biopsy was significantly associated with the risk of incomplete tumor resection (OR, 3.696; 95% CI, 1.528-8.938, P = 0.004). In 95.9% of the cases, it was possible to suspect a carcinoid tumor by macroscopic appearance during initial endoscopy. The preceding biopsy may disturb complete resection of rectal carcinoid tumor. In most cases, the carcinoid tumor could be suspected by macroscopic appearance. Therefore the preceding biopsy is not essential, and it may be avoided for the complete resection.

Graphical Abstract

INTRODUCTION

Carcinoid tumors are rare, slow-growing malignancies arising from enterochromaffin cells (1, 2). They can occur in nearly any gastrointestinal tissue; and rectum is one of the most commonly reported sites (3, 4, 5). According to a previous study, rectal carcinoids comprised 18.5% of all carcinoid tumors, and 27.4% of all gastrointestinal carcinoids (1). Carcinoid tumors of the rectum are likely increasing in incidence (1, 6).

Most rectal carcinoid tumors are small and movable subepithelial lesions located between 4 and 20 cm above the dentate line and are mainly discovered incidentally on routine lower endoscopy (7, 8, 9, 10). Such a small tumor rarely metastasizes and endoscopic resection is curative. Metastases to bone, lymph nodes and liver are only likely in tumors that are 2 cm and larger and they invade the muscularis propria (6, 7, 11). Overall distant metastases from rectal carcinoids occur in only 2.3%-3.9% (1, 3).

It is enough to remove a small lesion with the endoscopic treatment only, but it is important to resect completely (12, 13, 14). Any unsuccessfully resected lesions may need frequent follow-up or further treatment such as additional endoscopic treatment and operation.

Biopsy of carcinoid tumor is commonly taken before endoscopic resection. However most carcinoid tumors can be macroscopically distinguished by endoscopy, and a biopsy is not essential. In addition, biopsy can inhibit a complete resection by causing blurred tumor border and fibrosis of the tissue. Previous studies have revealed that the method of endoscopic treatment is an important factor in complete resection of rectal carcinoid tumor (12, 15). However little has been known about the effect of preceding biopsy on complete resection. Only one previous study suggested that the post-biopsy scar can interfere with successful endoscopic resection of rectal carcinoid tumor (16). However it had limited significance due to a small number of cases.

The primary objective of this study is to investigate the risk factors of incomplete tumor resection in rectal carcinoid tumor and to look into the effect of preceding biopsy on complete endoscopic resection. The secondary objective is to determine if rectal carcinoid tumors can be macroscopically distinguished by endoscopy.

MATERIALS AND METHODS

Patients and inclusion criteria

We reviewed retrospectively the records of patients with rectal subepithelial tumor who had undergone an endoscopic treatment at Konkuk University Hospital (Seoul, Korea), during a 7-yr period from July 2005 to July 2012. The data gathered on the patients and procedures were through endoscopic images, review of chart, and histologic results.

To be eligible for inclusion, the tumors had to be located in the rectum and be less than 20 mm in diameter. The endoscopic treatments which were included in the study were endoscopic submucosal dissection (ESD), endoscopic mucosal resection-band ligation (EMR-L), and conventional snare polypectomy. We excluded tumors resected by other methods, including hot biopsy, argon plasma coagulation (APC) and cold biopsy. Depending on the histological results, well-differentiated neuroendocrine tumors (grade I or II), namely carcinoid tumors, were included and other tumors were excluded. The tumors which involved muscularis propria, adjacent vessels, lymph nodes, or distant organ were additionally excluded. The cases with inadequate medical records were excluded as well. For patients with multiple rectal carcinoid tumors, each tumor removed by endoscopic treatment was categorized as a separate case.

Probable risk factors for incomplete tumor resection

According to completeness of resection, each tumor was classified into clear resection margin group (A group) and positive margin group (B group). When both the lateral and deep margins were free of tumor cells, it was denominated as complete resection. If it was unclear whether resection margin was involved, it was included in the positive margin group, as the subsequent therapeutic plan would be similar.

Probable risk factors for incomplete tumor resection were as follows: preceding biopsy before endoscopic resection; size of the tumor measured by endoscopist; location of tumor; morphology of tumor; and method of endoscopic treatment. In addition, we investigated the depth of invasion and whether endoscopic ultrasonography (EUS) was performed.

The location of tumor was divided into proximal and distal part of the rectum. Distal rectum was defined as the rectum located below the second valve of Houston. We used the diameter as measured by endoscopist during endoscopy rather than the diameter of specimen measured by pathologist, as part of the mass may have been removed by preceding biopsy. Also, in the positive resection margin group, a small portion of the residual tumor may have still remained on the resection site. Therefore the size of specimen as measured by pathologist may be smaller than the actual size.

The endoscopic morphology of tumor was divided into three types: protruded type; slightly elevated or flat type; and depressed or ulcerative type (Fig. 1). If biopsy was taken before the endoscopic resection, morphology after the preceding biopsy was selected as a risk factor of incomplete resection, as morphology of tumor may be transformed by biopsy. Describing tumor according to the Parris-Japanese classification, protruded type belonged to Ip, Isp and Is lesions, slightly elevated or flat type belonged to IIa and IIb lesions and depressed or ulcerative type were IIc and III lesions.

Fig. 1.

Color and endoscopic morphology of rectal carcinoid tumor. Among the 98 cases, tumors were yellow in 85 cases, white and pale in 10 cases, and the other three cases were similar in color to the surrounding normal mucosa. Tumor morphology after biopsy was classified into protruded type in 51 cases, slightly elevated or flat type in 43 and depressed or ulcerative type in 4. (A) The tumor is white and pale and morphologically belonged to the protruded type. (B) The color of tumor is yellow and it is of the slightly elevated type. (C) In the C-1 image, the tumor is a depressed type, and the color is neither yellow nor white. It could not be easily distinguished from the surrounding mucosa, but the tumor had definite capillary bed. C-2 image was the same patient's endoscopic image before the biopsy was taken. It shows typical features of carcinoid tumor, which was yellow-colored and slightly elevated type.

The endoscopic treatments were classified into three methods: ESD, EMR-L and conventional snare polypectomy. Simplified ESD which performed submucosal dissection with snaring was included in the ESD method.

Review of endoscopic records

In order to determine if carcinoid tumors were distinguished by endoscopy, we searched for the endoscopic records at the time of diagnosis. If the patient was referred from another hospital, we additionally reviewed the medical treatment request and endoscopic images of the previous hospital. Then we investigated whether "carcinoid" was written in the medical records before the patient was histologically confirmed to carcinoid tumor. In addition, we reviewed the records of patients who had rectal subepithelial tumor during the same study period and investigated the cases where the rectal tumor was recorded as "carcinoid" on medical record, but in fact were not.

We also reviewed the endoscopic images to see whether the shape and color of tumor were typical and if it looked like a subepithelial lesion, macroscopically. Review of the images was performed by two experienced endoscopists. If there were differing views, an agreement was reached through a discussion.

Endoscopic procedure

Standard colonoscope (CF-H260; Olympus, Tokyo, Japan) was used for the procedure. The colon was cleansed with 4 L polyethylene glycol electrolyte solution. Midazolam (Dormicum®) for conscious sedation and meperidine (Demerol®) for pain control were selectively given to patients. The procedure was performed in a standard fashion by one endoscopist and one assistant, and tumor removed following a saline injection mixed with indigocarmine and epinephrine. Conventional snare polypectomy was performed using oval-shaped snares (Olympus, Tokyo, Japan). Band ligation method was performed by standard upper endoscope (GF-260; Olympus) and pneumo-activate ELV device (Sumitomo Bakelite Co., Ltd., Tokyo, Japan). ESD was mostly carried out using a dual knife (KD-650L; Olympus).

Statistical analysis

Continuous variables were summarized as mean±standard deviation (SD), and categorical variables as frequency (%). To analyze the risk factors of incomplete tumor resection, categorical data were compared using chi-square or Fisher's exact tests. Then, we used logistic regression analysis to estimate the odds ratio (OR) and 95% confidence interval (CI) of risk factors that caused incomplete tumor resection. All analyses were conducted using SPSS for Windows (versions 19.0). A P value of less than 0.05 was considered statistically significant.

Ethics statement

The study was approved by the institutional review board of Konkuk University School of Medicine which confirmed that the study was in accordance with the ethical guidelines of the Helsinki Declaration (KUH1010502). All patients provided written consents to undergo endoscopic treatment and were informed of the risks and potential benefits of the procedures.

RESULTS

Characteristics of patients and tumors

Over the 7-yr period studied, 5,235 patients had undergone colonoscopic polypectomy, EMR or ESD in our unit. Among the patients, 113 patients were diagnosed with rectal subepithelial tumor and 16 of them were not carcinoid tumor. Therefore we identified 97 patients with rectal carcinoid who had undergone endoscopic tumor resection. There were three cases with no tumor in the resected tissue by endoscopic resection, although preceding biopsy revealed a carcinoid tumor. It may be that the small tumor was almost entirely resected by biopsy forceps, when the biopsy was performed. These three cases were excluded from the study, as they were not treated by the prescribed methods and the resection margin status could not be identified. In five patients, carcinoid tumor was found simultaneously at more than two locations. Four of these patients had two concurrent rectal carcinoids, and the other had one rectal carcinoid and one ampulla of Vater carcinoid. Thus, a total of 98 cases (94 patients) were finally included for the study (Fig. 2). The overall mean age and tumor size were 48.04±11.12 (range 29-75) yr and 7.40±2.86 (range 3-15) mm, respectively. Other characteristics of the tumors are shown in Table 1.

Fig. 2.

Selection of the cases. A total of 5,235 patients underwent endoscopic treatment of colonic tumor (polypectomy, EMR or ESD) at our hospital, during a 7-yr period from July 2005 to July 2012. Among them, we identified 113 patients with subepithelial tumor on rectum who had undergone endoscopic tumor resection. Finally, 98 rectal carcinoid cases (94 patients) were included, and the resection margin was clear in 57 cases.

Table 1.

Baseline characteristics of the patients and tumors

^*Continuous variables were summarized as mean±standard deviation (SD) and categorical variables as frequency (%); ^†The tumor size was measured by endoscopist during endoscopy. ESD, endoscopic submucosal dissection; EMR-L, endoscopic mucosal resection-band ligation; EUS, endoscopic ultrasonography.

Comparison of group A and B

The clear resection margin group (A group) and the positive margin group (B group) had 57 and 41 cases, respectively (Table 2). The B group included six cases where the margin status was unclear. Thirty five cases with definitely positive resection margin were composed of 18 deep margin positive cases, 1 lateral margin positive case, 2 both margin positive cases and 14 cases which were not marked separately.

Table 2.

Probable risk factors for incomplete resection of rectal carcinoid tumor and comparison of group A and B

^*Data were analyzed by chi-square test or Fisher's exact tests. Then, we used logistic regression analyses to estimate the odds ratio (OR) and 95% confidence intervals (CI). The significant results are written in bold; ^†Continuous variables were summarized as mean±standard deviation (SD) and categorical variables as frequency (%); ^‡The tumor size was measured by endoscopist during endoscopy. The A and B group indicate the clear resection margin group and the positive margin group, respectively. ESD, endoscopic submucosal dissection; EMR-L, endoscopic mucosal resection-band ligation.

The mean age of the A group was 49±12 (range 29-75) yr, and the mean tumor size was 7.26±2.83 (range 3-15) mm in diameter. The mean age of the B group was 47±10 (range 29-68), and the mean tumor size was 7.59±2.90 mm (range 3-15). A previous biopsy had been performed in 26 cases (45.6%) in group A and 31 cases (75.6%) in group B.

In group A, 21 (36.8%) tumors were removed by ESD, 28 (49.1%) tumors resected by snare polypectomy and 8 (14.0%) were EMR-L. In group B, ESD, snare polypectomy and EMR-L were performed in 13 (31.7%), 25 (61.0%) and 3 (7.3%) cases, respectively.

Tumor morphology before biopsy was divided into 57 protruded types and 39 slightly elevated or flat types. There was no depressed or ulcerative type. Two cases were not evaluated due to poor endoscopic images. However, tumor morphology after biopsy was changed into 51 protruded types, 43 slightly elevated or flat types, and 4 depressed or ulcerative types. Lesions confined to the mucosal layer were 2 in group A and 1 in group B. The rest had submucosal invasion. In both A and B groups, EUS was performed in 15 cases for each group. Of these several risk factors, only preceding biopsy was significantly associated with the risk of incomplete tumor resection (P=0.004, chi-square tests, Table 2). Logistic regression analysis revealed that patients who were performed preceding biopsy were about 3.7 times more likely to fail to completely remove tumors as compared to patients with naïve lesions (OR, 3.696; 95% CI, 1.528-8.938; P=0.004).

Identification of carcinoid tumor by initial endoscopy

All 98 carcinoid cases were observed as subepithelial lesion by the endoscopic images. Eighty five cases were yellow-colored tumor and ten cases were white and pale. Only three cases were similar in color to the surrounding normal mucosa. There were five cases which had definite capillary bed on tumor (Fig. 1). In all cases without biopsy and in 53 out of the 57 cases with preceding biopsy, "carcinoid" was written in the initial medical records before tumor was histologically confirmed as carcinoid. There were no unevaluated cases due to lack of the initial endoscopic record. Therefore, in 95.9% (94 cases) of all cases, carcinoid tumor could be suspected by macroscopic appearance at the time of the initial endoscopic examination.

There were five cases where the endoscopist attempted to remove the tumor with biopsy forceps, carcinoid tumor was not suspected at the time. In three of these cases, there was no residual tumor in the specimen obtained by additional polypectomy, and they were excluded from our study as stated earlier. The other two cases came under the four cases where "carcinoid" was not written in the initial medical records.

In three cases, although a preceding biopsy did not reveal a carcinoid tumor, a polypectomy was done from a strongly suspected carcinoid tumor macroscopically. These three cases were all diagnosed with carcinoid tumor, finally.

There were 16 patients who had rectal subepithelial tumor excepting carcinoid tumor in the study period. The histologic results were as follows: chronic nonspecific inflammation, inflammatory polyp, hyperplastic polyp, lymphoid polyp, leiomyoma, lipoma and malignant lymphoma (Fig. 2). Among these, according to the initial endoscopic records, there were six patients where the tumor looked like rectal carcinoid, but were not. None of the cases underwent EUS, and the final histologic results showed chronic nonspecific inflammation in three patients, lymphoid polyp in one, lipoma in one, and hyperplastic polyp in one (Fig. 3).

Fig. 3.

Rectal subepithelial tumors mimicking carcinoid tumor. There were six patients with tumor which looked to be rectal carcinoid, but were not. The final histologic results showed chronic nonspecific inflammation in 3 patients (A-C), lymphoid polyp (D), lipoma (E) and hyperplastic polyp (F). All of these patients did not undergo EUS. The retrospective review of the images showed that image C and F did not look like a subepithelial lesion.

Follow-up

In the B group, an evaluation was performed by patient's medical record from the timing of the polypectomy to October 2013. There has been no recurrence within the follow-up period and one patient underwent a surgical operation (lower anterior resection) due to the carcinoid tumor. Only 24 out of 41 patients were properly monitored. Their mean follow-up period was 33.29±24.75 (range 12-95) months and a first follow-up endoscopy and imaging studies were performed at 7.25±6.42 (range 2-24) months after the procedure. In one patient, follow-up study was performed at four months after the procedure, and then the patient did not visit the hospital for six years. The remaining 16 patients did not visit our outpatient gastroenterology clinic after the procedure. Two of them underwent health examination in our hospital after two and seven years, respectively, and there was no relapse.

DISCUSSION

Rectal carcinoids are diagnosed in relatively young patients in their 40s to 60s, and the incidence of multicentric carcinoids of the colon is low (1, 17). In our study, the mean age was 48 yr old and multicentric carcinoids were found in 5 out of 94 patients. These results were similar to the results of previous studies.

According to our results, preceding biopsy performed before endoscopic excision was the only factor of disturbing a complete resection of carcinoid tumor. The reasons for this are determined to be as follows. First, biopsy can make uncertain borders of tumors. It is important to localize the lesion to remove a carcinoid tumor, because carcinoid tumor is located in deeper layer than other polypoid lesions and the tumor is commonly small and subtle. If the lesion is flattened and margins blurred after biopsy, snaring and targeting of the lesion may be difficult. The second reason is tissue fibrosis after biopsy. Tissue fibrosis may disturb dissection in ESD procedure and suction in EMR-L.

Previous studies revealed that pathologically complete resection of small rectal carcinoid tumors was more likely to be achieved using advanced endoscopic techniques rather than conventional polypectomy (12, 15, 18). The complete resection rates were 30.9%, 72.0%, and 81.8% for conventional polypectomy, advanced endoscopic techniques and surgical local excision, respectively (15). Another study showed that the complete resection rates were 20.0% by conventional polypectomy, 84.6% by two-channel endoscopic mucosal resection and 77.8% by ESD (12). In our study, such tendency was also observed, but the result of ESD method was not as good as expected. The reasons for this may be that it is very difficult to dissect the submucosal layer of small lesions below 1 cm in size. It is also technically difficult to resect a tumor which is located in distal rectum. In addition, previous results of ESD method were not adjusted for the effect of preceding biopsy. In general, preceding biopsy tends to be taken less often before ESD than other methods due to the concern for tissue fibrosis. However biopsy was frequently done before ESD in our study. This may be one reason for low complete resection rate of rectal carcinoid tumor treated by ESD in our study.

Rectal carcinoid tumor has many distinguishable endoscopic features. Endoscopic findings of carcinoid tumor are characterized by smooth, round and sessile elevations covered with yellow and pale colored mucosa (19). The tumor is located in deep mucosa and submucosa, and is usually movable and hard (3, 20). In our study, almost every carcinoid lesion was also observed as a yellowish or whitish subepithelial tumor, except in three cases. To add to these findings, it is imperative to make accurate diagnosis by checking for hardness and movability of tumor using forceps. In 95.9% of all our cases, carcinoid tumor could be suspected by macroscopic appearance during initial endoscopy. If diagnosis is difficult by these findings only, EUS may be helpful in the diagnosis and treatment plan. Among the six patients who were misdiagnosed, there was no one who underwent EUS. In addition, the retrospective review of the endoscopic images showed that two of six tumors did not look like a subepithelial lesion (Fig. 3C and F). Therefore careful observation by experienced endoscopist may lower the rate of misdiagnosis.

Our study had some limitations. First, if a detailed record was omitted by the endoscopist, it would have caused spurious results, as our study was based on the medical records. Over the 7-yr study period, there were six cases where the tumors looked like rectal carcinoid, but were not. However we assume that there may have been more cases lacking formal records of endoscopic findings. In addition, among the cases where "carcinoid" was not written in the initial medical records, it was unclear whether the endoscopists had suspected carcinoid tumor or not. Second, there may be some misdiagnoses, as the initial endoscopic impressions are founded on the interpretations of individual endoscopist. According to review of the endoscopic images, some of the lesions which were classified subepithelial tumor seemed to not be such (Fig. 3). Third, in many cases, we could not sort the resection margin status into lateral and deep margin positive due to poor pathologic record and fragmentation of the specimen. However categorization of the lateral or deep margin involvement may be important, as lateral and deep margin involvement can occur for different reasons. Therefore, in the future, well-designed prospective studies are required for separate analysis of the lateral and deep margin involvement. Forth limitation is a lack of data on the prognosis of the B group. There was no recurrence within the follow-up period, however the period of follow-up was relatively short and there were a lot of losses to follow-up. Rectal carcinoid tumor is slow-growing tumor. Therefore long-term follow-up study is needed to overcome this limitation.

In conclusion, biopsy of rectal carcinoid tumor may have to be avoided. Most carcinoid tumors can be macroscopically distinguished by endoscopy, and biopsy can inhibit a complete resection. Incompletely resected lesions may need frequent follow-up endoscopy and/or further treatments. As a result, it can increase additional medical costs and cause needless suffering. At this time, biopsy of rectal carcinoid tumor is commonly taken before endoscopic resection. According to our study, a preceding biopsy was taken in 41.8% of the cases. Therefore we cautiously suggest that biopsy of rectal carcinoid tumor should not be taken in the field for the complete resection of tumor, especially if the tumor has macroscopically distinguishable features. However, in proof of this, more research must be done.