Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1996 Mar 5;93(5):2122–2124. doi: 10.1073/pnas.93.5.2122

Expression of protein encoded by varicella-zoster virus open reading frame 63 in latently infected human ganglionic neurons.

R Mahalingam 1, M Wellish 1, R Cohrs 1, S Debrus 1, J Piette 1, B Rentier 1, D H Gilden 1
PMCID: PMC39920  PMID: 8700895

Abstract

The ganglionic cell type in which varicella-zoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV open reading frame 63 protein. VZV open reading frame 63 protein was detected exclusively in the cytoplasm of neurons of latently infected human trigeminal and thoracic ganglia. This is, to our knowledge, the first identification of a herpesvirus protein expressed during latency in the human nervous system.

Full text

PDF
2122

Images in this article

2123Fig. 1
on p.2123

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clarke P., Beer T., Cohrs R., Gilden D. H. Configuration of latent varicella-zoster virus DNA. J Virol. 1995 Dec;69(12):8151–8154. doi: 10.1128/jvi.69.12.8151-8154.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohrs R. J., Barbour M. B., Mahalingam R., Wellish M., Gilden D. H. Varicella-zoster virus (VZV) transcription during latency in human ganglia: prevalence of VZV gene 21 transcripts in latently infected human ganglia. J Virol. 1995 Apr;69(4):2674–2678. doi: 10.1128/jvi.69.4.2674-2678.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohrs R. J., Srock K., Barbour M. B., Owens G., Mahalingam R., Devlin M. E., Wellish M., Gilden D. H. Varicella-zoster virus (VZV) transcription during latency in human ganglia: construction of a cDNA library from latently infected human trigeminal ganglia and detection of a VZV transcript. J Virol. 1994 Dec;68(12):7900–7908. doi: 10.1128/jvi.68.12.7900-7908.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Croen K. D., Ostrove J. M., Dragovic L. J., Straus S. E. Patterns of gene expression and sites of latency in human nerve ganglia are different for varicella-zoster and herpes simplex viruses. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9773–9777. doi: 10.1073/pnas.85.24.9773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Debrus S., Sadzot-Delvaux C., Nikkels A. F., Piette J., Rentier B. Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency. J Virol. 1995 May;69(5):3240–3245. doi: 10.1128/jvi.69.5.3240-3245.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilden D. H., Dueland A. N., Cohrs R., Martin J. R., Kleinschmidt-DeMasters B. K., Mahalingam R. Preherpetic neuralgia. Neurology. 1991 Aug;41(8):1215–1218. doi: 10.1212/wnl.41.8.1215. [DOI] [PubMed] [Google Scholar]
  7. Gilden D. H., Rozenman Y., Murray R., Devlin M., Vafai A. Detection of varicella-zoster virus nucleic acid in neurons of normal human thoracic ganglia. Ann Neurol. 1987 Sep;22(3):377–380. doi: 10.1002/ana.410220315. [DOI] [PubMed] [Google Scholar]
  8. Gilden D. H., Vafai A., Shtram Y., Becker Y., Devlin M., Wellish M. Varicella-zoster virus DNA in human sensory ganglia. Nature. 1983 Dec 1;306(5942):478–480. doi: 10.1038/306478a0. [DOI] [PubMed] [Google Scholar]
  9. Gilden D. H., Wright R. R., Schneck S. A., Gwaltney J. M., Jr, Mahalingam R. Zoster sine herpete, a clinical variant. Ann Neurol. 1994 May;35(5):530–533. doi: 10.1002/ana.410350505. [DOI] [PubMed] [Google Scholar]
  10. Hyman R. W., Ecker J. R., Tenser R. B. Varicella-zoster virus RNA in human trigeminal ganglia. Lancet. 1983 Oct 8;2(8354):814–816. doi: 10.1016/s0140-6736(83)90736-5. [DOI] [PubMed] [Google Scholar]
  11. Mahalingam R., Wellish M., Brucklier J., Gilden D. H. Persistence of varicella-zoster virus DNA in elderly patients with postherpetic neuralgia. J Neurovirol. 1995 Mar;1(1):130–133. doi: 10.3109/13550289509111018. [DOI] [PubMed] [Google Scholar]
  12. Mahalingam R., Wellish M., Lederer D., Forghani B., Cohrs R., Gilden D. Quantitation of latent varicella-zoster virus DNA in human trigeminal ganglia by polymerase chain reaction. J Virol. 1993 Apr;67(4):2381–2384. doi: 10.1128/jvi.67.4.2381-2384.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mahalingam R., Wellish M., Wolf W., Dueland A. N., Cohrs R., Vafai A., Gilden D. Latent varicella-zoster viral DNA in human trigeminal and thoracic ganglia. N Engl J Med. 1990 Sep 6;323(10):627–631. doi: 10.1056/NEJM199009063231002. [DOI] [PubMed] [Google Scholar]
  14. McGeoch D. J., Dolan A., Donald S., Rixon F. J. Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. J Mol Biol. 1985 Jan 5;181(1):1–13. doi: 10.1016/0022-2836(85)90320-1. [DOI] [PubMed] [Google Scholar]
  15. Meier J. L., Holman R. P., Croen K. D., Smialek J. E., Straus S. E. Varicella-zoster virus transcription in human trigeminal ganglia. Virology. 1993 Mar;193(1):193–200. doi: 10.1006/viro.1993.1115. [DOI] [PubMed] [Google Scholar]
  16. Merville-Louis M. P., Sadzot-Delvaux C., Delrée P., Piette J., Moonen G., Rentier B. Varicella-zoster virus infection of adult rat sensory neurons in vitro. J Virol. 1989 Jul;63(7):3155–3160. doi: 10.1128/jvi.63.7.3155-3160.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ramakrishnan R., Levine M., Fink D. J. PCR-based analysis of herpes simplex virus type 1 latency in the rat trigeminal ganglion established with a ribonucleotide reductase-deficient mutant. J Virol. 1994 Nov;68(11):7083–7091. doi: 10.1128/jvi.68.11.7083-7091.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sadzot-Delvaux C., Merville-Louis M. P., Delrée P., Marc P., Piette J., Moonen G., Rentier B. An in vivo model of varicella-zoster virus latent infection of dorsal root ganglia. J Neurosci Res. 1990 May;26(1):83–89. doi: 10.1002/jnr.490260110. [DOI] [PubMed] [Google Scholar]
  19. Stevens J. G., Wagner E. K., Devi-Rao G. B., Cook M. L., Feldman L. T. RNA complementary to a herpesvirus alpha gene mRNA is prominent in latently infected neurons. Science. 1987 Feb 27;235(4792):1056–1059. doi: 10.1126/science.2434993. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES