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. 2014 Apr 10;142(1):78–88. doi: 10.1111/imm.12234

Figure 1.

Figure 1

Experimental protocols. Rats were divided into five groups: non-sensitized control group (NS); Aspergillus fumigatus spore-exposed group (NS + AF); ovalbumin (OVA)-sensitized and OVA-challenged group (OVA); OVA-sensitized, OVA-challenged and A. fumigatus spore-exposed group (OVA + AF); and CRTH2 antagonist OC00459-treated group (OVA + AF + Treat). In the OVA-sensitized and OVA-challenged receiving saline (OVA) group, rats were sensitized by two intraperitoneal injections of OVA/aluminium hydroxide and then received three consecutive days of an aerosolized OVA challenge from days 14 to 37 for 4 weeks. To evaluate the effect of A. fumigatus, OVA-sensitized and OVA-challenged mice received 5 consecutive days of an aerosol inhalation of 1 × 106 colony-forming unit/ml spore suspension from days 42 to 67 for 4 weeks. The OVA + AF + Treat group received OC000459 at a dose of 5 mg/kg daily. Non-sensitized and non-challenged mice receiving saline or A. fumigatus represented the NS and NS + AF groups, respectively. Values are means±SEM. *P < 0·05.