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. Author manuscript; available in PMC: 2015 May 1.

Published in final edited form as: Xenotransplantation. 2014 Mar 20;21(3):244–253. doi: 10.1111/xen.12091

(A) The usage of the immunoglobulin heavy chain gene IGHV3-66*02 was elevated from 7%±0% in pretransplant baboon serum to 35%±6% post-islet cell transplantation. (B) The nucleotide sequence of four representative IGV_H genes encoding IgM anti-nonGal xenoantibodies is shown. The IGV_H genes encoding anti-nonGal porcine islet xenoantibodies is shown compared to the sequence of the IGHV3-21*01 germline gene which encodes anti-nonGal xenoantibodies to GTKO pig endothelial cells without multiple genetic modifications. (C) The amino acid sequence of IGV_H genes encoding anti-nonGal xenoantibodies to genetically modified pig islets is shown. The regions of the CDR1 and CDR2 which are conserved may be relevant for contact with nonGal xenoantigen. (−) Identical residues when compared with the closest human germline gene. FWR (framework region) CDR (complementary determining region).

(A) The usage of the immunoglobulin heavy chain gene IGHV3-66*02 was elevated from 7%±0% in pretransplant baboon serum to 35%±6% post-islet cell transplantation. (B) The nucleotide sequence of four representative IGV_H genes encoding IgM anti-nonGal xenoantibodies is shown. The IGV_H genes encoding anti-nonGal porcine islet xenoantibodies is shown compared to the sequence of the IGHV3-21*01 germline gene which encodes anti-nonGal xenoantibodies to GTKO pig endothelial cells without multiple genetic modifications. (C) The amino acid sequence of IGV_H genes encoding anti-nonGal xenoantibodies to genetically modified pig islets is shown. The regions of the CDR1 and CDR2 which are conserved may be relevant for contact with nonGal xenoantigen. (−) Identical residues when compared with the closest human germline gene. FWR (framework region) CDR (complementary determining region).